Harpin-induced expression and transgenic overexpression of the phloem protein gene AtPP2-A1 in Arabidopsis repress phloem feeding of the green peach aphid Myzus persicae

Chunling Zhang; Haojie Shi; Lei Chen; Xiaomeng Wang; Beibei Lü; Shuping Zhang; Yuan Liang; Ruoxue Liu; Jun Qian; Weiwei Sun; Zhenzhen You; Hansong Dong
January 2011
BMC Plant Biology;2011, Vol. 11 Issue 1, p1
Academic Journal
Background: Treatment of plants with HrpNEa, a protein of harpin group produced by Gram-negative plant pathogenic bacteria, induces plant resistance to insect herbivores, including the green peach aphid Myzus persicae, a generalist phloem-feeding insect. Under attacks by phloem-feeding insects, plants defend themselves using the phloem-based defense mechanism, which is supposed to involve the phloem protein 2 (PP2), one of the most abundant proteins in the phloem sap. The purpose of this study was to obtain genetic evidence for the function of the Arabidopsis thaliana (Arabidopsis) PP2-encoding gene AtPP2-A1 in resistance to M. persicae when the plant was treated with HrpNEa and after the plant was transformed with AtPP2-A1. Results: The electrical penetration graph technique was used to visualize the phloem-feeding activities of apterous agamic M. persicae females on leaves of Arabidopsis plants treated with HrpNEa and an inactive protein control, respectively. A repression of phloem feeding was induced by HrpNEa in wild-type (WT) Arabidopsis but not in atpp2-a1/E/142, the plant mutant that had a defect in the AtPP2-A1 gene, the most HrpNEa-responsive of 30 AtPP2 genes. In WT rather than atpp2-a1/E/142, the deterrent effect of HrpNEa treatment on the phloem-feeding activity accompanied an enhancement of AtPP2-A1 expression. In PP2OETAt (AtPP2-A1-overexpression transgenic Arabidopsis thaliana) plants, abundant amounts of the AtPP2-A1 gene transcript were detected in different organs, including leaves, stems, calyces, and petals. All these organs had a deterrent effect on the phloem-feeding activity compared with the same organs of the transgenic control plant. When a large-scale aphid population was monitored for 24 hours, there was a significant decrease in the number of aphids that colonized leaves of HrpNEatreated WT and PP2OETAt plants, respectively, compared with control plants. Conclusions: The repression in phloem-feeding activities of M. persicae as a result of AtPP2-A1 overexpression, and as a deterrent effect of HrpNEa treatment in WT Arabidopsis rather than the atpp2-a1/E/142 mutant suggest that AtPP2-A1 plays a role in plant resistance to the insect, particularly at the phloem-feeding stage. The accompanied change of aphid population in leaf colonies suggests that the function of AtPP2-A1 is related to colonization of the plant.


Related Articles

  • An open and shut case. Molloy, Sheilagh // Nature Reviews Microbiology;Nov2006, Vol. 4 Issue 11, p804 

    The article presents a study which determines the reaction of Arabidopsis thaliana stomata with the plant pathogen Pseudomonas syringae pv. tomato DC300 (PstDC300). It has been noted tha bacterial pathogens cannot pass through the plant tissues directly, hence, they depend on the openings of the...

  • Impact of Initial Pathogen Density on Resistance and Tolerance in a Polymorphic Disease Resistance Gene System in Arabidopsis thaliana. Roux, Fabrice; Liping Gao; Bergelson, Joy // Genetics;May2010, Vol. 185 Issue 1, p283 

    The evolution of natural enemy defense shapes evolutionary trajectories of natural populations. Although the intensity of selection imposed by enemies clearly varies among natural populations, little is known about the reaction norm of genotypes under a gradient of selective pressure. In this...

  • The ELF4 gene controls circadian rhythms and flowering time in Arabidopsis thaliana. Doyle, Mark R.; Davis, Seth J.; Bastow, Ruth M.; McWatters, Harriet G.; Kozma-Bognár, László; Nagy, Ferenc; Millar, Andrew J.; Amasino, Richard M. // Nature;9/5/2002, Vol. 419 Issue 6902, p74 

    Describes the early flowering 4 (ELF4) gene and its role in the control of the circadian rhythm and flowering time of the Arabidopsis thaliana. Promotion of clock accuracy and sustained rhythms in the absence of daily light/dark cycles; Function of the circadian clock associated 1 gene expressed...

  • Light responses of a plastic plant. Millar, Andrew J. // Nature Genetics;Dec2001, Vol. 29 Issue 4, p357 

    Arabidopsis thaliana isolates from the wild vary enormously in their morphology and physiological responses to standard conditions, indicating that substantial genetic variation is accessible in this species. Natural variant alleles encoding two Arabidopsis photoreceptors, phytochrome A and...

  • Plant genetics: a decade of integration. Pruitt, Robert E.; Bowman, John L.; Grossniklaus, Ueli // Nature Genetics;Mar2003 Supplement, Vol. 33, p294 

    The last decade provided the plant science community with the complete genome sequence of Arabidopsis thaliana and rice, tools to investigate the function of potentially every plant gene, methods to dissect virtually any aspect of the plant life cycle, and a wealth of information on gene...

  • Heterogeneous Selection at Specific Loci in Natural Environments in Arabidopsis thaliana. Weinig, Cynthia; Dorn, Lisa A.; Kane, Nolan C.; German, Zachary M.; Halldorsdottir, Solveig S.; Ungerer, Mark C.; Toyonaga, Yuko; Mackay, Trudy F.c.; Purugganan, Michael D.; Schmitt, Johanna // Genetics;Sep2003, Vol. 165 Issue 1, p321 

    Reports that selection on quantitative trait loci (QTL) for fitness traits in the model plant species Arabidopsis thaliana differs among natural ecological settings and genetic backgrounds. Suggestion that the allelic fitness effects of two QTL reversed direction depending on the genotype at...

  • Genetic Architecture of Plastic Methyl Jasmonate Responses in Arabidopsis thaliana. Kliebenstein, Daniel J.; Figuth, Antje; Mitchell-Olds, Thomas // Genetics;Aug2002, Vol. 161 Issue 4, p1685 

    Examines the genetic architecture of plastic methyl jasmonate (MeJa) responses in Arabidopsis thaliana. Analysis of MeJa and salicylic acid cross-talk in glucosinolate regulation; Implications for glucosinolate physiology and functional understanding of Arabidopsis MeJa signal transduction.

  • Genetic variation within and among populations of Arabidopsis thaliana. Bergelson, Joy; Stahl, Eli; Dudek, Scott; Kreitman, Martin // Genetics;Mar98, Vol. 148 Issue 3, p1311 

    Presents a study which examined the genetic variation of Arabidopsis thaliana. Examination of the levels of nucleotide polymorphism within Arabidopsis thaliana; Method employed in this study; Results of this study.

  • HrpN-induced deterrent effect on phloem feeding of the green peach aphid Myzus persicae requires AtGSL5 and AtMYB44 genes in Arabidopsis thaliana. Lü, Beibei; Weiwei Sun; Shuping Zhang; Chunling Zhang; Jun Qian; Xiaomeng Wang; Rong Gao; Hansong Dong // Journal of Biosciences;Mar2011, Vol. 36 Issue 1, p123 

    In Arabidopsis thaliana ( Arabidopsis) treated with the harpin protein HrpN, resistance to the green peach aphid Myzus persicae, a generalist phloem-feeding insect, develops with induced expression of the AtMYB44 gene. Special GLUCAN SYNTHESIS-LIKE ( GSL) genes and β-1,3-glucan callose play...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics