Prime immunization with rotavirus VLP 2/6 followed by boosting with an adenovirus expressing VP6 induces protective immunization against rotavirus in mice

Hongli Zhou; Li Guo; Min Wang; Jianguo Qu; Zhendong Zhao; Jianwei Wang; Tao Hung
January 2011
Virology Journal;2011, Vol. 8 Issue 1, p1
Academic Journal
Background: Rotavirus (RV) is the main cause of severe gastroenteritis in children. An effective vaccination regime against RV can substantially reduce morbidity and mortality. Previous studies have demonstrated the efficacy of virus-like particles formed by RV VP2 and VP6 (VLP2/6), as well as that of recombinant adenovirus expressing RV VP6 (rAd), in eliciting protective immunities against RV. However, the efficacy of such prime-boost strategy, which incorporates VLP and rAd in inducing protective immunities against RV, has not been addressed. We assessed the immune effects of different regimens in mice, including rAd prime-VLP2/6 boost (rAd+VLP), VLP2/6 prime-rAd boost (VLP+rAd), rAd alone, and VLP alone. Results: Mice immunized with the VLP+rAd regimen elicit stronger humoral, mucosal, and cellular immune responses than those immunized with other regimens. RV challenging experiments showed that the highest reduction (92.9%) in viral shedding was achieved in the VLP+rAd group when compared with rAd+VLP (25%), VLP alone (75%), or rAd alone (40%) treatment groups. The reduction in RV shedding in mice correlated with fecal IgG (r = 0.95773, P = 0.04227) and IgA (r = 0.96137, P = 0.038663). Conclusions: A VLP2/6 prime-rAd boost regimen is effective in conferring immunoprotection against RV challenge in mice. This finding may lay the groundwork for an alternative strategy in novel RV vaccine development.


Related Articles

  • Rotarix in Developing Countries: Paving the Way for Inclusion in National Childhood Immunization Programs in Africa. Pawinski, Robert; Debrus, Serge; Delem, Andrée; Smolenov, Igor; Suryakiran, Pemmaraju V.; Han, Htay Htay // Journal of Infectious Diseases;9/1/2010 Supplement, Vol. 202, pS80 

    Rotavirus gastroenteritis causes more than half a million deaths annually among children aged <5 years, the great majority of which occur in Africa and Asia. Vaccination is considered to be the most effective public health strategy to prevent rotavirus disease and to reduce the significant...

  • A prospective evaluation of community acquired gastroenteritis in paediatric practices: impact and disease burden of rotavirus infection. Frühwirth, M.; Karmaus, W.; Moll-Schüler, I.; Brösl, S.; Mutz, I. // Archives of Disease in Childhood;May2001, Vol. 84 Issue 5, p393 

    Aims--To examine the disease burden and epidemiology of community acquired rotavirus gastroenteritis in Austrian children treated in a paediatric practice. Methods--A prospective, population based, multicentre study in four paediatric practices and two children's hospitals (Innsbruck and...

  • Primary care-based surveillance to estimate the burden of rotavirus gastroenteritis among children aged less than 5 years in six European countries. Diez-Domingo, Javier; Baldo, Jose-Maria; Patrzalek, Marian; Pazdiora, Petr; Forster, Johannes; Cantarutti, Luigi; Pirçon, Jean-Yves; Soriano-Gabarró, Montse; Meyer, Nadia; Pirçon, Jean-Yves; Soriano-Gabarró, Montse; SPRIK Rotavirus Study Group // European Journal of Pediatrics;Feb2011, Vol. 170 Issue 2, p213 

    This observational, prospective study was undertaken to estimate the burden of rotavirus (RV) gastroenteritis (GE) leading to general practitioner (GP)/family paediatrician (FP) visits among children aged <5 years in Czech Republic, Germany, Italy, Poland, Spain and the UK. Children aged <5...

  • Comportamiento clínico de 70 casos de gastroenteritis por rotavirus, en lactantes previamente inmunizados. Reyes Hernández, Diana Piedad; Reyes Hernández, Katy Lizeth; Reyes Gómez, Ulises; Pérez Pacheco, Ofelia; Angelo Quiroz, Michael; Reyes Hernández, Ulises // Revista Mexicana de Pediatria;nov/dic2014, Vol. 81 Issue 6, p214 

    In a prospective, observational and cross-sectional study was analyzed the clinical and laboratory behavior of 70 cases of gastroenteritis by rotavirus in infants previously immunized with 2 doses of Rotarix at 2 and 4 months. The diagnosis of the cases was performed by positive fecal latex. 42...

  • Timely immunisation of premature infants against rotavirus in the neonatal intensive care unit. Ladhani, Shamez N.; Ramsay, Mary E. // Archives of Disease in Childhood -- Fetal & Neonatal Edition;Nov2014, Vol. 99 Issue 6, pF445 

    The article discusses the steps taken the government of Great Britain in July 2013 to introduce live-attenuated rotavirus vaccine Rotarix into the national infant immunisation programme. Topics discussed include premature born babies not protected against rotavirus infection due to poor passive...

  • ROTA...The Round Virus. Monaco, John E. // Pediatrics for Parents;2004, Vol. 21 Issue 10, p6 

    Focuses on the rotavirus infections that causes gastroenteritis in children. Clinical history of the children with the infection; Factors that determines dehydration in children; Signs and symptoms; Importance fluids as treatment for dehydration.

  • Infant rotavirus vaccine protected older children, adults. Shane, Andi L. // Infectious Diseases in Children;Oct2013, Vol. 26 Issue 10, p18 

    The article discusses the use of infant rotavirus vaccine to reduce gastroenteritis discharges among unvaccinated older children and adults according to a study findings published in "JAMA."

  • GASTROENTERITIS: Rotavirus vaccination reduces hospitalisations.  // Pulse;8/22/2012, Vol. 72 Issue 27, p10 

    The article presents information on the findings of a study according to which Rotavirus vaccination is as effective in clinical practice for reducing hospital admissions of gastroenteritis in children.

  • Providing Early Protection Against Rotavirus Gastroenteritis. Congeni, Blase L. // Contemporary Pediatrics;Aug2009 Providing Early Protection, p2 

    The article offers clinical information on rotavirus gastroenteritis (RGE). It describes the burden of rotavirus disease in the U.S., along with the clinical features of RGE, the impact of immunization and the cost of RGE. Results of the clinical studies on Rotarix vaccine for rotavirus are...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics