Spatial and temporal deposition of suberin during maturation of the onion root exodermis

Meyer, Chris J.; Peterson, Carol A.; Bernards, Mark A.
February 2011
Botany;Feb2011, Vol. 89 Issue 2, p119
Academic Journal
Suberin is a complex biopolymer composed of a poly(aliphatic) domain (SPAD) and a poly(phenolic) domain (SPPD). Suberin is typically confined to specialized cell types including root exodermal cells, but its synthesis in a maturing exodermis is still not well understood. For the current work, Allium cepa roots were used as a model to analyze SPAD and SPPD synthesis in a maturing uniseriate exodermis. Roots were divided into four maturation zones based on their growth rate and the deposition of suberin lamellae in maturing exodermal cells. Exodermal and epidermal cell layers were separated from the underlying layers in each maturation zone, then soluble and insoluble suberin monomers were extracted chemically, and quantified and identified by gas chromatography - mass spectrometry. Temporal patterns for the synthesis of the SPAD, but not for the SPPD, were revealed upon resolution of the metabolite profiles. The composition of the soluble fraction was essentially unchanged as the exodermis matured. In contrast, the SPAD composition differed during maturation, mainly owing to significant increases in the deposition of C18:1 α,ω-dioic acid and ω-OH fatty acids. It is proposed that the exodermal maturation zones with their corresponding metabolite profiles be used as targets for the functional enzymatic characterization of suberin biosynthetic pathways. La subérine est un biopolymère complexe composé d'un domaine (SPAD) poly(aliphatique) et d'un domaine (SPPD) poly(phénolique). Typiquement, la subérine se confine à des types de cellules spécialisées, incluant les cellules exodermiques des racines, mais on ne comprend toujours pas très bien sa synthèse dans l'exoderme en maturation. Pour ce travail, les auteurs ont utilisé la racine de l'Allium cepa comme modèle afin d'analyser la synthèse du SPAD et du SPDD dans un exoderme unisérié en maturation. Ils ont divisé les racines en quatre zones de maturation, basées sur leur taux de croissance et sur la déposition des lamelles de subérine dans les cellules exodermiques en maturation. Ils ont séparé les couches cellulaires épidermiques et exodermiques des couches sous-jacentes pour chaque zone de maturation. Ensuite ils ont extrait chimiquement les monomères de subérine solubles et insolubles, avant de les identifier et les quantifier par le masse couplée à la chromatographie en phase gazeuse. Suite à la résolution des profils métaboliques, les auteurs ont pu révéler des patrons temporels pour la synthèse du SPAD, mais pas du SPPD. La composition de la fraction soluble demeure essentiellement inchangée au fil de la maturation de l'exoderme. Au contraire, la composition du SPAD diffère au cours de la maturation, surtout dûà des augmentations significatives de la déposition d'acide dioïque C18:1 α,ω et des acides gras ω-OH. On propose d'utiliser les zones de maturation exodermiques avec leurs profils métaboliques, comme cibles pour la caractérisation enzymatique fonctionnelle des sentiers métaboliques de subérine.


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