TITLE

Recombination technologies for enhanced transgene stability in bioengineered insects

AUTHOR(S)
Schetelig, Marc; Götschel, Frank; Viktorinová, Ivana; Handler, Alfred; Wimmer, Ernst
PUB. DATE
January 2011
SOURCE
Genetica;Jan2011, Vol. 139 Issue 1, p71
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Transposon-based vectors currently provide the most suitable gene transfer systems for insect germ-line transformation and are used for molecular improvement of the Sterile Insect Technique. However, the long time stability of genome-integrated transposon constructs depends on the absence of transposase activity that could remobilize the transposon-embedded transgenes. To achieve transgene stability transposon vectors are usually non-autonomous, lacking a functional transposase gene, and chosen so that endogenous or related transposon activities are not present in the host. Nevertheless, the non-autonomous transposon-embedded transgenes could become unstable by the unintended presence of a mobilizing transposase that may have been undetected or subsequently entered the host species by horizontal gene transfer. Since the field release of transgenic insects will present environmental concerns relating to large populations and high mobility, it will be important to ensure that transgene constructs are stably integrated for maintaining strain integrity and eliminating the possibility for unintentional transfer into the genome of another organism. Here we review efficient methods to delete or rearrange terminal repeat sequences of transposons necessary for their mobility, subsequent to their initial genomic integration. These procedures should prevent transposase-mediated remobilization of the transgenes, ensuring their genomic stability.
ACCESSION #
57639750

 

Related Articles

  • home style. Griffin, Christine Kidder // New Orleans Magazine;Jul2000, Vol. 34 Issue 10, p66 

    Answers several questions about pest control in homes and gardens. Approach to exterminating bugs around the home; Methods that can be used to control insects; Information on the organic products or home remedies to keep pest insects away.

  • Grub talk. SHETLAR, DAVE // Landscape Management;Sep2013, Vol. 52 Issue 9, p35 

    The article presents questions and answers related to grubs including the state of bug infestation in Texas and Florida, the probability of grub infestation in shaded areas and how recurring grub problems may be addressed.

  • Safe and fit genetically modified insects for pest control: from lab to field applications. Scolari, F.; Siciliano, P.; Gabrieli, P.; Gomulski, L.; Bonomi, A.; Gasperi, G.; Malacrida, A. // Genetica;Jan2011, Vol. 139 Issue 1, p41 

    Insect transgenesis is continuously being improved to increase the efficacy of population suppression and replacement strategies directed to the control of insect species of economic and sanitary interest. An essential prerequisite for the success of both pest control applications is that the...

  • Whole genome sequencing in Drosophila virilis identifies Polyphemus, a recently activated Tc1-like transposon with a possible role in hybrid dysgenesis. Blumenstiel, Justin P. // Mobile DNA;2014, Vol. 5 Issue 1, p1 

    Background Hybrid dysgenic syndromes in Drosophila have been critical for characterizing host mechanisms of transposable element (TE) regulation. This is because a common feature of hybrid dysgenesis is germline TE mobilization that occurs when paternally inherited TEs are not matched with a...

  • Grapefruit cocktail beats bad bugs!  // Organic Gardening (08973792);Jan93, Vol. 40 Issue 1, p18 

    Discusses a two-year study at the University of Maine that reveals that grapefruit seeds can be a potent zapper of Colorado potato beetles and fairly good at repelling Mexican bean bettles, corn earworms and fall armyworms. Chemical limonin, extracted from ground and dried citrus seeds;...

  • Bugproof Without DEET.  // Backpacker;Jun99, Vol. 27 Issue 5, p100 

    Suggests several options on how to ward off bugs without using repellents containing DEET.

  • Leaping Libanasidus! Johannesburg 'burbs invaded by odd bug. Matoff, Judith // Christian Science Monitor;1/23/95, Vol. 87 Issue 39, p6 

    Informs on the invasion of the Johannesburg suburbs by `Libanasidus vittatus' or the Parktown Prawn bugs. Description and origin of the bugs; Concern of the citizens; Views of an entomologist on their behavior, control and spread.

  • Fighting houseplant pests. Sanchez, Janet H.; Sears, Elayne // Horticulture;Nov92, Vol. 70 Issue 9, p24 

    Presents a step-by-step guide to keep insects at bay. How to isolate the infested plant; Wash the plant; Spray the plant; Remain vigilant; How to prevent future infestations; Details.

  • Repellence. Allen, Oliver E. // Horticulture;Apr93, Vol. 71 Issue 4, p12 

    Offers suggestions for virtually foolproof protection against the nightly attack of winged assailants. Mosquitoes, blackflies, fleas, chiggers and ticks; Products to use; Synthetic chemical repellents; Natural remedies; How the sprays work; Details.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics