TITLE

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF RISPERIDONE AND TRIHEXYPHENIDYL HYDROCHLORIDE IN TABLET DOSAGE FORMS

AUTHOR(S)
Patel, Dhara; Patel, Jayvadan
PUB. DATE
September 2010
SOURCE
International Journal of Pharmaceutical Sciences Review & Resear;Sep2010, Vol. 4 Issue 3, p85
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A binary mixture of Risperidone (RIS) and Trihexyphenidyl hydrochloride (THP) was determined using reversed-phase liquid chromatography method using methanol: acetonitrile: 0.05 M phosphate buffer (pH 3.7) (60:30:10, v/v/v) pumped at a flow rate of 1.0 ml/min. Quantification was achieved with ultraviolet detection at 230 nm over concentration ranges of 0.5-5.0 and 1.0-10.0 μg/ml; mean accuracies were 100.55±0.64 and 100.75±0.81%, respectively. The method was successively applied to tablet dosage forms as no chromatographic interferences from the tablet excipients were observed. The method retained its accuracy and precision when the standard addition technique was applied.
ACCESSION #
57621855

 

Related Articles

  • The Effect of Modifier on Selectivity in Reversed-Phase High Performance Liquid Chromatography. Klimek-Turek, Anna; Dzido, Tadelz H.; Engelhardt, Heinz // LC-GC Europe;Jan2008, Vol. 21 Issue 1, p33 

    The retention behavior of several compounds has been compared for their selectivity using reversed-phase high performance liquid chromatography with binary water mobile phases composed of methanol, acetonitrile or tetrahydrofuran as modifiers.

  • Determination of Guaifensen and Dextromethorphan by UPLC. Suneetha, G.; Venkateswarlu, P.; Prasad, P. S. S. // Der Pharma Chemica;2012, Vol. 4 Issue 3, p1019 

    A new HPLC method was developed for selective and simultaneous determination of Guaifensen and Dextromethorphan HBr tablets 1200/60 mg. The chromatographic separation was achieved on a Acquity UPLC BEH, C18,100 × 2.1 mm, 1.7um column. The mobile phase consisted of buffer, acetonitrile and...

  • Development and validation of a RP-HPLC Method for Simultaneous Estimation of Atenolol and Nitrendipine in Tablet Dosage Form. Kumudhavalli, M. V.; Babu, K. Anand; Jayakar, B. // Der Pharma Chemica;2011, Vol. 3 Issue 4, p63 

    A rapid high performance liquid chromatographic method has been developed and validated for the estimation of Atenolol and Nitrendipine simultaneously in combined dosage form. A Phenomenox C-18 column having dimensions of 4.6x250 mm and particle size of 5 µm in isocratic mode, with mobile...

  • Development of RP-HPLC method for estimation of Olmesartan Medoxomil in tablet dosage forms. M. K., Chaitanya prasad; G, Vidyasagar; K. R. S., Sambasiva Rao; S., Ramanjeneyulu // Der Pharma Chemica;2011, Vol. 3 Issue 6, p208 

    Reversed phase high performance liquid chromatographic method was developed and validated for the estimation of Olmesartan medoxomil in bulk and formulation. Selected mobile phase was a combination of phosphate buffer with pH adjusted at 2.8 and acetonitrile (35:65% v/v) and wavelength selected...

  • Stability Indicating HPLC Determination of Risperidone in Bulk Drug and Pharmaceutical Formulations. Dedania, Zarna R.; Dedania, Ronak R.; Sheth, Navin R.; Patel, Jigar B.; Patel, Bhavna // International Journal of Analytical Chemistry;2011, Vol. 2011, p1 

    The objective of the current study was to develop a validated stability-indicating assay method (SIAM) for risperidone after subjecting it to forced decomposition under hydrolysis, oxidation, photolysis, and thermal stress conditions. The liquid chromatographic separation was achieved...

  • Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Montelukast Sodium and Ebastine in Tablet Dosage Form. RANA, N. S.; RAJESH, K. S.; PATEL, NIKITA N.; PATEL, P. R.; LIMBACHIYA, U.; PASHA, T. Y. // Indian Journal of Pharmaceutical Sciences;Sep/Oct2013, Vol. 75 Issue 5, p599 

    A rapid and sensitive RP-HPLC method with UV detection (244 nm) for routine analysis of montelukast sodium and ebastine in a pharmaceutical formulation (Ebast-M) was developed. Chromatography was performed with mobile phase containing a mixture of methanol:acetonitrile:ammonium acetate...

  • What Drives a Separation--the Stationary Phase or the Mobile Phase? Wysocki, Jessica // LC-GC North America;Nov2001, Vol. 19 Issue 11, p1150 

    The driving force of a liquid chromatography separation is determined by solute partitioning between the mobile and stationary phases. Chromatographers must study a wide range of mobile-phase compositions and stationary-phase bonding densities to determine the thermodynamic driving force. The...

  • New RP-HPLC Method Development and Validation for Estimation of Tapentadol hydrochloride in Bulk and Pharmaceutical Dosage form. Runja, Chinnalalaiah; Pigili, Ravikumar // Journal of Pharmacy Research;2012, Vol. 5 Issue 12, p5489 

    A simple, rapid, selective, precise and accurate new RP-HPLC method was developed and validated for assay of Tapentadol Hydrochloride in bulk and tablet dosage form. The method was developed by using the solvent system, Phosphate Buffer: Methanol: Acetonitrile in the ratio of 50:20:30 (v/v/v)...

  • Selectivity in Reversed-Phase LC Separations, Part I: Solvent- Type Selectivity. Dolan, John W. // LC-GC North America;Dec2010, Vol. 28 Issue 12, p1022 

    The article discusses the significance of solvent selectivity triangle in reversed-phase liquid chromatography (LC) separations wherein methanol and acetonitrile are the most popular organic solvents used in the experiment. It notes the popular application of the solvent selectivity triangle...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics