TITLE

The epididymis-specific antimicrobial peptide β-defensin 15 is required for sperm motility and male fertility in the rat ( Rattus norvegicus)

AUTHOR(S)
Zhao, Yue; Diao, Hua; Ni, Zimei; Hu, Shuanggang; Yu, Heguo; Zhang, Yonglian
PUB. DATE
February 2011
SOURCE
Cellular & Molecular Life Sciences;Feb2011, Vol. 68 Issue 4, p697
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Spermatozoa acquire forward motility and fertilizing capacity during their transit through the epididymis. The maturation process involves modifications of the sperm surface by different proteins that are secreted by a series of specialized regions in the epididymal epithelium. Here we show that the rat epididymis-specific β-defensin 15 ( Defb15) exhibits an androgen-dependent expression pattern, and it can bind to the acrosomal region of caput sperm. Coculture of caput spermatozoa with Defb15 antibody in vitro resulted in a significant decline in sperm motility. Moreover, the total and progressive motility of the spermatozoa dramatically decreased in rats when Defb15 was downregulated by lentivirus-mediated RNAi in vivo. Remarkably, knock down of Defb15 led to a reduction in fertility and embryonic development failure. In addition, the recombinant Defb15 showed antimicrobial activity in a dose-dependent fashion. These results suggest that Defb15 plays a dual role in both sperm maturation and pathogen defense in rat epididymis.
ACCESSION #
57580462

 

Related Articles

  • Conference Abstracts . . . May 13, 2002.  // Fertility Weekly;5/13/2002, p21 

    Presents abstracts related to fertility and human reproductive science. "Mistletoe Therapy in Endometriosis," by Y. T. Lim; "Time Elapsed from Initial Complaints to the Diagnosis of Endometriosis in a Cohort of Brazilian Women," by C.A. Petta, M. Arruda, M. Abrao and C. Laguna; "Is ATP...

  • Cryopreserved Human Spermatozoa Post-Thaw Motility Enhanced.  // Fertility Weekly;8/19/2002, p13 

    Pentoxifylline had a positive influence on cryopreserved human sperm only when added after thawing, according to researchers in Croatia. Although sperm cryopreservation has become an important component of assisted reproduction, the freeze-thaw process results in reduced motility, viability, and...

  • The association between polypronucleate zygote formation with certain motion characteristics of sperm and IVF outcome. Tsung-Hsien Lee; Chung-Hsien Liu; Chun-Chia Huang; Hsui-Hui Chen; Shee-Uan Chen; Maw-Sheng Lee // Journal of Assisted Reproduction & Genetics;Jan2008, Vol. 25 Issue 1, p35 

    Purpose To determine the efficiency of sperm motion characteristics as predictors for normal (2PN) and polypronulceate (PPN) zygotes in IVF. Methods A retrospective cohort analysis for a total of 230 couples undergoing IVF treatment in a single infertility center. Result(s) Subsequent to semen...

  • Enhancement of mouse sperm motility by the PI3-kinase inhibitor LY294002 does not result in toxic effects on preimplantation embryo development. Michaela Luconi; Simona Torcia; Domenico Grillo; Maria Teresa Fiorenza; Gianni Forti; Franco Mangia; Elisabetta Baldi // Human Reproduction;Dec2005, Vol. 20 Issue 12, p3500 

    BACKGROUND: A reduced number of progressively motile sperm (as may occur in cases of asthenozoospermia or when cryopreserved spermatozoa are used for fertilization) limits the possibility of applying various assisted reproductive techniques (ARTs). We previously showed that incubation of sperm...

  • Changes of mice testicular sperms membrane glycocalyx after exposure to L-carnitine and Pentoxifylline. Aliabadi, E.; Karimi, F.; Talaei-Khozani, T. // Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p112 

    Introduction: The glycoconjugate content of sperms indicates their physiological and fertility properties. Lectin reactivity is indicative of intact, capacitated, and acrosome-reacted sperms. In the epididymis, sperms experience maturation, glycoconjugate modification, and simultaneously, higher...

  • Host Defence (Cationic) Peptides: What Is Their Future Clinical Potential? Hancock, R.E.W. // Drugs;Apr1999, Vol. 57 Issue 4, p469 

    Host defence, cationic antimicrobial peptides are now recognised as an important component, in most species, of the early innate and induced defences against invading microbes. They are small (12 to 35 amino acids), cationic due to the presence of an excess of arginine and lysine over acidic...

  • Clinical pregnancy increases with testicular sperm. Schieszer, John // Urology Times;Apr2003, Vol. 31 Issue 4, p11 

    Reports on a study showing that clinical pregnancy and implantation appear to be increased in couples using testicular sperm compared with using suboptimal ejaculates from the same male partners. Determination of which sperm origin produces more favorable fertilization and pregnancy outcomes in...

  • Resources.  // GP: General Practitioner;3/21/2008, p32 

    The article lists several sources related to subfertility in men including "Management of infertility," "European Association of Urology guidelines on male infertility," and "Human fertilization and Embryology Authority."

  • Evolutionary sperm wars. Parker, Geoff // Journal of Biological Education (Society of Biology);Autumn97, Vol. 31 Issue 3, p167 

    Probes into physiological and behavioral adaptations related to sperm competition or the natural raffle which occurs when sperm from two or more male animals compete to fertilize a particular set of eggs. Behavioral strategies of ground squirrels; Model of a random role sperm competition;...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics