TITLE

Role of capsaicin-sensitive primary afferent neurons and non-protein sulphydryl groups on gastroprotective effect of amifostine against ethanol-induced gastric damage in rats

PUB. DATE
February 2011
SOURCE
Digestive Diseases & Sciences;Feb2011, Vol. 56 Issue 2, p314
SOURCE TYPE
Academic Journal
DOC. TYPE
journal article
ABSTRACT
Background: Amifostine has been widely tested as a cytoprotective agent against a number of aggressors in different organs. Recently, a gastroprotective effect was observed for this drug in a model of indomethacin-induced gastric injury. Our objective was to investigate the effect of amifostine on ethanol-induced gastric injury and the role played in this mechanism by afferent sensory neurons, non-protein sulfhydryl groups, nitric oxide, ATP-sensitive potassium channels, and cyclooxygenase-2.Methods: Rats were treated with amifostine (22.5, 45, 90, or 180 mg/kg, PO or SC). After 30 min, the rats received absolute ethanol (5 ml kg(-1), PO). One hour later, gastric damage was quantified with a planimeter. Samples from the stomach were also taken for histopathological assessment and for assays of non-protein sulfhydryl groups. The other groups were pretreated with L-NAME (10 mg kg(-1), IP), glibenclamide (10 mg kg(-1), PO), or celecoxib (10 mg kg(-1), PO). After 30 min, the animals were given amifostine (90 mg kg(-1), PO or SC), followed 30 min later by gavage with absolute ethanol (5 ml kg(-1)). Other rats were desensitized with capsaicin (125 mg kg(-1), SC) 8 days prior to amifostine treatment.Results: Amifostine administration PO and SC significantly and dose-dependently reduced ethanol-induced macroscopic and microscopic gastric damage by restoring glutathione levels in the stomach mucosa. Amifostine-promoted gastroprotection against ethanol-induced stomach injury was reversed by pretreatment with neurotoxic doses of capsaicin, but not by L-NAME, glibenclamide, or celecoxib.Conclusions: Amifostine protects against ethanol-induced gastric injury by increasing glutathione levels and stimulating the afferent sensory neurons in the stomach.
ACCESSION #
57580437

 

Related Articles

  • Phenotype of distinct primary sensory afferent subpopulations and caspase-3 expression following axotomy. Reid, Adam; Mantovani, Cristina; Shawcross, Susan; Terenghi, Giorgio; Wiberg, Mikael // Histochemistry & Cell Biology;Jul2011, Vol. 136 Issue 1, p71 

    Specific sensory neuronal subpopulations show contrasting responses to peripheral nerve injury, as shown by the axotomy-induced death of many cutaneous sensory neurons whilst muscular sensory afferents survive an identical insult. We used a novel combination of retrograde neuronal tracing with...

  • And motion changes it all. Ahissar, Ehud // Nature Neuroscience;Dec2008, Vol. 11 Issue 12, p1369 

    The article focuses on a research that demonstrated the mechanism of the sensory processing under an active motor system in the rat vibrissal system. It states that the researchers show that sensory processing is different than sensory processing under an inactive motor system. Based on the...

  • TRPV1 Marks Synaptic Segregation of Multiple Convergent Afferents at the Rat Medial Solitary Tract Nucleus. Peters, James H.; McDougall, Stuart J.; Fawley, Jessica A.; Andresen, Michael C. // PLoS ONE;2011, Vol. 6 Issue 9, p1 

    TRPV1 receptors are expressed on most but not all central terminals of cranial visceral afferents in the caudal solitary tract nucleus (NTS). TRPV1 is associated with unmyelinated C-fiber afferents. Both TRPV1+ and TRPV1- afferents enter NTS but their precise organization remains poorly...

  • Electrical activity of the sensory afferent pathway in the enteric nervous system. Miftakhov, R. N.; Wingate, D. L. // Biological Cybernetics;1996, Vol. 75 Issue 6, p471 

    In this paper we develop a mathematical model for the electrical activity of the afferent pathway, formed from the coupled primary and secondary sensory neurons. The primary sensory neuron possesses the electrical properties of AH neurons and morphological characteristics of Dogiel type II...

  • Cholecystokinin enhances visceral pain-related affective memory via vagal afferent pathway in rats.  // Molecular Brain;2012, Vol. 5 Issue 1, p19 

    The article presents a study which measures the learned behavior reflecting the affective component of visceral pain through vagal afferent pathway in rats. It demonstrate that perigenual anterior cingulate cortex (pACC) activation is important in memory processing in long-term visceral...

  • Changes in TRPV1-Immunoreactive Neurons in Spinal Nerve Sensory Ganglion Neurons in Rats on Exposure to Capsaicin. Porseva, V.; Shilkin, V.; Korzina, M.; Korobkin, A.; Maslyukov, P. // Neuroscience & Behavioral Physiology;Sep2012, Vol. 42 Issue 7, p770 

    The morphological characteristics of TRPV1-immunoreactive neurons were studied in thoracic spinal nerve sensory ganglia in rats aged 10, 20, 30, 60, and 90 days in normal conditions and after chemical deafferentation by s.c. administration of capsaicin (100 mg/kg) on the second day of life....

  • Calcium-dependent inhibition of T-type calcium channels by TRPV1 activation in rat sensory neurons. Comunanza, Valentina; Carbone, Emilio; Marcantoni, Andrea; Sher, Emanuele; Ursu, Daniel // Pflugers Archiv European Journal of Physiology;Nov2011, Vol. 462 Issue 5, p709 

    We studied the inhibitory effects of transient receptor potential vanilloid-1 (TRPV1) activation by capsaicin on low-voltage-activated (LVA, T-type) Ca channel and high-voltage-activated (HVA; L, N, P/Q, R) currents in rat DRG sensory neurons, as a potential mechanism underlying...

  • Differential Regulation of ASICs and TRPV1 by Zinc in Rat Bronchopulmonary Sensory Neurons. Vysotskaya, Zhanna; Moss, Charles; Gu, Qihai // Lung;Dec2014, Vol. 192 Issue 6, p927 

    Purpose: Zinc has been known to act as a signaling molecule that regulates a variety of neuronal functions. In this study, we aimed to study the effect of zinc on two populations of acid-sensitive ion channels, acid-sensing ion channels (ASICs), and transient receptor potential vanilloid...

  • Identifying a primary mechanotransducer in a model sensory system. Suslak, T.; Jarman, A. P.; Armstrong, D. // Proceedings of the Physiological Society;2014, p64P 

    An abstract of the article "Identifying a primary mechanotransducer in a model sensory system" by T. Suslak, A. P. Jarman and D. Armstrong is presented.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics