Mapping of Tonoplast Intrinsic Proteins in Maturing and Germinating Arabidopsis Seeds Reveals Dual Localization of Embryonic TIPs to the Tonoplast and Plasma Membrane

Gattolin, Stefano; Sorieul, Mathias; Frigerio, Lorenzo
January 2011
Molecular Plant (Oxford University Press / USA);Jan2011, Vol. 4 Issue 1, p180
Academic Journal
We have mapped the expression of the tonoplast intrinsic protein (TIP) gene family members in Arabidopsis seeds by fluorescent protein tagging of their genomic sequences and confocal microscopy. Three isoforms (TIP1;1, TIP2;1, and TIP2;2) have distinct patterns of expression in maternal tissues (outer integument and placento-chalazal region). Two isoforms, TIP3;1 and the previously uncharacterized TIP3;2, are the only detectable TIPs in embryos during seed maturation and the early stages of seed germination. Throughout these developmental stages, both isoforms co-locate to the tonoplast of the protein storage vacuoles, but also appear to label the plasma membrane. Plasma membrane labeling is specific to TIP3;1 and TIP3;2, is independent of the position of the fluorescent protein tag, and appears to be specific to early seed maturation and early germination stages. We discuss these results in the context of the predicted distribution of aquaporins in Arabidopsis seeds.


Related Articles

  • LysoPC acyltransferase/PC transacylase activities in plant plasma membrane and plasma membrane-associated endoplasmic reticulum. Larsson, Karin E.; Kjellberg, J. Magnus; Tjellström, Henrik; Sandelius, Anna Stina // BMC Plant Biology;2007, Vol. 7, p64 

    Background: The phospholipids of the plant plasma membrane are synthesized in the endoplasmic reticulum (ER). The majority of these lipids reach the plasma membrane independently of the secretory vesicular pathway. Phospholipid delivery to the mitochondria and chloroplasts of plant cells also...

  • Tonoplast intrinsic proteins and vacuolar identity. Gattolin, Stefano; Sorieul, Mathias; Frigerio, Lorenzo // Biochemical Society Symposia;Jun2010, Vol. 38 Issue 3, p769 

    TIPs (tonoplast intrinsic proteins) have been traditionally used as markers for vacuolar identity in a variety of plant species and tissues. In the present article, we review recent attempts to compile a detailed map of TIP expression in Arabidopsis, in order to understand vacuolar identity and...

  • A Small Molecule Inhibitor Partitions Two Distinct Pathways for Trafficking of Tonoplast Intrinsic Proteins in Arabidopsis. Rivera-Serrano, Efrain E.; Rodriguez-Welsh, Maria F.; Hicks, Glenn R.; Rojas-Pierce, Marcela; Van Damme, Els J. M. // PLoS ONE;Sep2012, Vol. 7 Issue 9, Special section p1 

    Tonoplast intrinsic proteins (TIPs) facilitate the membrane transport of water and other small molecules across the plant vacuolar membrane, and members of this family are expressed in specific developmental stages and tissue types. Delivery of TIP proteins to the tonoplast is thought to occur...

  • Arabidopsis sterol carrier protein-2 is required for normal development of seeds and seedlings. Bing Song Zheng; Rönnberg, Elin; Viitanen, Lenita; Salminen, Tiina A.; Lundgren, Krister; Moritz, Thomas; Edqvist, Johan // Journal of Experimental Botany;Sep2008, Vol. 59 Issue 12, p3485 

    The Arabidopsis thaliana sterol carrier protein-2 (AtSCP2) is a small, basic and peroxisomal protein that in vitro enhances the transfer of lipids between membranes. AtSCP2 and all other plant SCP-2 that have been identified are single-domain polypeptides, whereas in many other eukaryotes SCP-2...

  • Subcellular localization of endogenous IAA during poplar leaf rhizogenesis revealed by in situ immunocytochemistry. Dong, Ningguang; Gao, Ying; Hao, Yanbin; Yin, Weilun; Pei, Dong // Plant Biotechnology Reports;Sep2014, Vol. 8 Issue 5, p377 

    Poplar 741 [ Populus alba × ( P. davidiana + P. simonii) × P. tomentosa] leaves were rooted within 8 days when cultured on 1/2 MS medium. The subcellular localization of endogenous indole-3-acetic acid (IAA) in the rhizogenesis was investigated, using an immunocytochemical approach. The...

  • Aquaporin NIP2;1 is Mainly Localized to the ER Membrane and Shows Root-Specific Accumulation in Arabidopsis thaliana. Masahiro Mizutani; Satoshi Watanabe; Tsuyoshi Nakagawa; Masayoshi Maeshima // Plant & Cell Physiology;Oct2006, Vol. 47 Issue 10, p1420 

    We investigated a nodulin 26-like protein NIP2;1, which belongs to the third subgroup of Arabidopsis aquaporins. Histochemical analysis of a promoter–β-glucuronidase fusion revealed the root-specific expression of NIP2;1. The NIP2;1 protein was detected in young roots, but not in...

  • O-Carboxyl- and N-Methyltransferases Active on Plant Aquaporins. Sahr, Tobias; Adam, Thibaud; Fizames, Cécile; Maurel, Christophe; Santoni, Véronique // Plant & Cell Physiology;Dec2010, Vol. 51 Issue 12, p2092 

    Methylation of biologically active molecules is achieved by methyltransferases (MTases). MTases can act on proteins through N- or O-carboxylmethylation reactions. Methylation of lysine and glutamic acid residues was recently described on the N-terminal tail of AtPIP2;1, a plasma membrane...

  • Functional Characterization of Arabidopsis Calreticulin1a: A Key Alleviator of Endoplasmic Reticulum Stress. Anna Christensen; Karin Svensson; Staffan Persson; Joanna Jung; Marek Michalak; Susanne Widell; Marianne Sommarin // Plant & Cell Physiology;Jun2008, Vol. 49 Issue 6, p912 

    The chaperone calreticulin plays important roles in a variety of processes in the endoplasmic reticulum (ER) of animal cells, such as Ca2+ signaling and protein folding. Although the functions of calreticulin are well characterized in animals, only indirect evidence is available for plants. To...

  • An improved high-throughput screening assay for tunicamycin sensitivity in Arabidopsis seedlings. McCormack, Maggie E.; Xiaoyu Liu; Jordan, Melissa R.; Pajerowska-Mukhtar, Karolina M. // Frontiers in Plant Science;Aug2015, p1 

    Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA Tunicamycin (Tm) sensitivity assays are a useful method for studies of endoplasmic reticulum stress and the unfolded protein response in eukaryotic cells. While Tm sensitivity and Tm recovery assays have been...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics