Arabidopsis brassinosteroid biosynthetic mutant dwarf7-1 exhibits slower rates of cell division and shoot induction

Jinyeong Cheon; So-Young Park; Schulz, Burkhard; Choe, Sunghwa
January 2010
BMC Plant Biology;2010, Vol. 10, p270
Academic Journal
Background: Plant growth depends on both cell division and cell expansion. Plant hormones, including brassinosteroids (BRs), are central to the control of these two cellular processes. Despite clear evidence that BRs regulate cell elongation, their roles in cell division have remained elusive. Results: Here, we report results emphasizing the importance of BRs in cell division. An Arabidopsis BR biosynthetic mutant, dwarf7-1, displayed various characteristics attributable to slower cell division rates. We found that the DWARF4 gene which encodes for an enzyme catalyzing a rate-determining step in the BR biosynthetic pathways, is highly expressed in the actively dividing callus, suggesting that BR biosynthesis is necessary for dividing cells. Furthermore, dwf7-1 showed noticeably slower rates of callus growth and shoot induction relative to wild-type control. Flow cytometric analyses of the nuclei derived from either calli or intact roots revealed that the cell division index, which was represented as the ratio of cells at the G2/M vs. G1 phases, was smaller in dwf7-1 plants. Finally, we found that the expression levels of the genes involved in cell division and shoot induction, such as PROLIFERATING CELL NUCLEAR ANTIGEN2 (PCNA2) and ENHANCER OF SHOOT REGENERATION2 (ESR2), were also lower in dwf7-1 as compared with wild type. Conclusions: Taken together, results of callus induction, shoot regeneration, flow cytometry, and semi-quantitative RT-PCR analysis suggest that BRs play important roles in both cell division and cell differentiation in Arabidopsis.


Related Articles

  • Functional characterization of Arabidopsis thaliana transthyretin-like protein. Pessoa, João; Sárkány, Zsuzsa; Ferreira-da-Silva, Frederico; Martins, Sónia; Almeida, Maria R.; Jianming Li; Damas, Ana M. // BMC Plant Biology;2010, Vol. 10, p30 

    Background: Arabidopsis thaliana transthyretin-like (TTL) protein is a potential substrate in the brassinosteroid signalling cascade, having a role that moderates plant growth. Moreover, sequence homology revealed two sequence domains similar to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline...

  • DIMINUTO 1 affects the lignin profile and secondary cell wall formation in Arabidopsis. Hossain, Zakir; McGarvey, Brian; Amyot, Lisa; Gruber, Margaret; Jung, Jinwook; Hannoufa, Abdelali // Planta;Mar2012, Vol. 235 Issue 3, p485 

    Brassinosteroids (BRs) play a crucial role in plant growth and development and DIMINUTO 1 (DIM1), a protein involved in BR biosynthesis, was previously identified as a cell elongation factor in Arabidopsis thaliana. Through promoter expression analysis, we showed that DIM1 was expressed in most...

  • The Mechanisms of Brassinosteroids' Action: From Signal Transduction to Plant Development. Yang, Cang-Jin; Zhang, Chi; Lu, Yang-Ning; Jin, Jia-Qi; Wang, Xue-Lu // Molecular Plant (Oxford University Press / USA);Jul2011, Vol. 4 Issue 4, p588 

    Brassinosteroids play diverse roles in plant growth and development. Plants deficient in brassinosteroid (BR) biosynthesis or defective in signal transduction show many abnormal developmental phenotypes, indicating the importance of both BR biosynthesis and the signaling pathway in regulating...

  • Interaction Between Photoreceptors and BR Signaling in Arabidopsis. Zhu, Zhong Xin; Zhu, Xiao Feng; Zhu, Yu Ting; Yao, Da Nian; Xuan, Yuan Hu // Acta Biologica Cracoviensia Series Botanica;2014, Vol. 56 Issue 2, p126 

    Extensive studies have been performed to elucidate the role of brassinosteroids (BRs), an important class of phy-tohormone in plant growth, development, and photomorphogenesis. Different wavelengths of light recognized by photoreceptors play a crucial role in plant development. The role of...

  • Brassinosteroid signaling network: implications on yield and stress tolerance. Hao, Jingjie; Yin, Yanhai; Fei, Shui-zhang // Plant Cell Reports;Jul2013, Vol. 32 Issue 7, p1017 

    The steroidal hormone brassinosteroids (BRs) play important roles in plant growth and development. Genetic, genomic and proteomic studies in Arabidopsis have identified major BR signaling components and elucidated the signal transduction pathway from the cell surface receptor kinase BRI1 to the...

  • Enhancing Brassinosteroid Signaling via Overexpression of Tomato (Solanum lycopersicum) SlBRI1 Improves Major Agronomic Traits. Shuming Nie; Shuhua Huang; Shufen Wang; Dandan Cheng; Jianwei Liu; Siqi Lv; Qi Li; Xiaofeng Wang // Frontiers in Plant Science;8/10/2017, p1 

    Brassinosteroids (BRs) play important roles in plant growth, development, and stress responses through the receptor, Brassinosteroid-insensitive 1 (BRI1), which perceives BRs and initiates BR signaling. There is considerable potential agricultural value in regulating BR signaling in crops. In...

  • Differential expression of the brassinosteroid receptor-encoding BRI1 gene in Arabidopsis. Hategan, Lidia; Godza, Blanka; Kozma-Bognar, Laszlo; Bishop, Gerard; Szekeres, Miklos // Planta;May2014, Vol. 239 Issue 5, p989 

    Brassinosteroid (BR)-regulated growth and development in Arabidopsis depends on BRASSINOSTEROID INSENSITIVE 1 (BRI1), the BR receptor that is responsible for initiating the events of BR signalling. We analysed the temporal and spatial regulation of BRI1 expression using stable transgenic lines...

  • Brassinosteroids promote Arabidopsis pollen germination and growth. Vogler, Frank; Schmalzl, Christina; Englhart, Maria; Bircheneder, Martin; Sprunck, Stefanie // Plant Reproduction;Sep2014, Vol. 27 Issue 3, p153 

    Pollen tubes are among the fastest tip-growing plant cells and represent an excellent experimental system for studying the dynamics and spatiotemporal control of polarized cell growth. However, investigating pollen tube tip growth in the model plant Arabidopsis remains difficult because in vitro...

  • Expression of plastid genome and development of Arabidopsis thaliana with disturbed synthesis of brassinosteroids. Efimova, M.; Kusnetsov, V.; Kravtsov, A.; Bartashevich, D.; Karnachuk, R.; Kovtun, I.; Kuznetsov, Vl. // Russian Journal of Plant Physiology;Jan2012, Vol. 59 Issue 1, p28 

    Comparative analysis of growth characteristics, content of auxins and cytokinins, and the level of photosynthetic pigments was conducted in det2 mutant plants of Arabidopsis thaliana (L.) Heynh notable for a disturbed synthesis of brassinosteroids and therefore their low endogenous level. In...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics