TITLE

Genetic structure and ecogeographical adaptation in wild barley (Hordeum chilense Roemer et Schultes) as revealed by microsatellite markers

AUTHOR(S)
Castillo, Almudena; Dorado, Gabriel; Feuillet, Catherine; Sourdille, Pierre; Hernandez, Pilar
PUB. DATE
January 2010
SOURCE
BMC Plant Biology;2010, Vol. 10, p266
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Multi-allelic microsatellite markers have become the markers of choice for the determination of genetic structure in plants. Synteny across cereals has allowed the cross-species and cross-genera transferability of SSR markers, which constitute a valuable and cost-effective tool for the genetic analysis and marker-assisted introgression of wild related species. Hordeum chilense is one of the wild relatives with a high potential for cereal breeding, due to its high crossability (both interspecies and intergenera) and polymorphism for adaptation traits. In order to analyze the genetic structure and ecogeographical adaptation of this wild species, it is necessary to increase the number of polymorphic markers currently available for the species. In this work, the possibility of using syntenic wheat SSRs as a new source of markers for this purpose has been explored. Results: From the 98 wheat EST-SSR markers tested for transferability and polymorphism in the wild barley genome, 53 primer pairs (54.0%) gave cross-species transferability and 20 primer pairs (20.4%) showed polymorphism. The latter were used for further analysis in the H. chilense germplasm. The H. chilense-Triticum aestivum addition lines were used to test the chromosomal location of the new polymorphic microsatellite markers. The genetic structure and diversity was investigated in a collection of 94 H. chilense accessions, using a set of 49 SSR markers distributed across the seven chromosomes. Microsatellite markers showed a total of 351 alleles over all loci. The number of alleles per locus ranged from two to 27, with a mean of 7.2 alleles per locus and a mean Polymorphic Information Content (PIC) of 0.5. Conclusions: According to the results, the germplasm can be divided into two groups, with morphological and ecophysiological characteristics being key determinants of the population structure. Geographic and ecological structuring was also revealed in the analyzed germplasm. A significant correlation between geographical and genetic distance was detected in the Central Chilean region for the first time in the species. In addition, significant ecological influence in genetic distance has been detected for one of the population structure groups (group II) in the Central Chilean region. Finally, the association of the SSR markers with ecogeographical variables was investigated and one marker was found significantly associated with precipitation. These findings have a potential application in cereal breeding.
ACCESSION #
57220351

 

Related Articles

  • Identification and genetic characterization of anAegilops tauschiiortholog of the wheat leaf rust disease resistance geneLr1. Hong-Qing Ling; Jiwen Qiu; Singh, Ravi; Keller, Beat // Theoretical & Applied Genetics;Oct2004, Vol. 109 Issue 6, p1133 

    Aegilops tauschii(goat grass) is the progenitor of the D genome in hexaploid bread wheat. We have screened more than 200Ae. tauschiiaccessions for resistance against leaf rust (Puccinia triticina) isolates, which are avirulent on the leaf rust resistance geneLr1. Approximately 3.5% of theAe....

  • Molecular cytogenetic characterization of Roegneria ciliaris chromosome additions in common wheat. Wang, X. E.; Chen, P. D.; Liu, D. J.; Zhang, P.; Zhou, B.; Friebe, B.; Gill, B. S. // Theoretical & Applied Genetics;Apr2001, Vol. 102 Issue 5, p651 

    The development of alien addition lines is important both for transferring useful genes from related species into common wheat and for studying the relationship between alien chromosomes and those of wheat. Roegneria ciliaris (2n=4x=28, ScScYcYc) is reported to be a potential source of...

  • Identification and mapping of molecular markers linked to the tuberculate fruit gene in the cucumber ( Cucumis sativus L.). Weiwei Zhang; Huanle He; Yuan Guan; Hui Du; Lihua Yuan; Zheng Li; Danqing Yao; Junsong Pan; Run Cai // Theoretical & Applied Genetics;Feb2010, Vol. 120 Issue 3, p645 

    Warty fruit is one of the highly valuable external quality traits related to the market values of cucumber. Genetic analysis has shown that a single dominant gene, Tu (Tuberculate fruit), determines the warty fruit trait in the cucumber plant. An F2 population (247 individuals) from the cross of...

  • Unexpanded and intermediate CAG polymorphisms at the SCA2 locus (ATXN2) in the Cuban population: evidence about the origin of expanded SCA2 alleles. Laffita-Mesa, José Miguel; Velázquez-Pérez, Luis C; Santos Falcón, Nieves; Cruz-Mariño, Tania; González Zaldívar, Yanetza; Vázquez Mojena, Yaimee; Almaguer-Gotay, Dennis; Almaguer Mederos, Luis Enrique; Rodríguez Labrada, Roberto // European Journal of Human Genetics;Jan2012, Vol. 20 Issue 1, p41 

    The role of short, large or intermediate normal alleles (ANs) of the ataxin-2 gene in generating expanded alleles (EAs) causing spinocerebellar ataxia type 2 (SCA2) is poorly understood. It has been postulated that SCA2 prevalence is related to the frequency of large ANs. SCA2 shows the highest...

  • A new cacao linkage map based on codominant markers: development and integration of 201 new microsatellite markers. Pugh, T.; Fouet, O.; Risterucci, A. M.; Brottier, P.; Abouladze, M.; Deletrez, C.; Courtois, B.; Clement, D.; Larmande, P.; N'Goran, J. A. K.; Lanaud, C. // Theoretical & Applied Genetics;Apr2004, Vol. 108 Issue 6, p1151 

    A linkage map of cacao based on codominant markers has been constructed by integrating 201 new simple sequence repeats (SSR) developed in this study with a number of isoenzymes, restriction fragment length polymorphisms (RFLP), microsatellite markers and resistance and defence gene analogs...

  • Reduced Variation on the Chicken Z Chromosome. Sundström, Hannah; Webster, Matthew T.; Ellegren, Hans // Genetics;May2004, Vol. 167 Issue 1, p377 

    Understanding the population genetic factors that shape genome variability is pivotal to the design and interpretation of studies using large-scale polymorphism data. We analyzed patterns of polymorphism and divergence at Z-linked and autosomal loci in the domestic chicken (Gallus gallus) to...

  • Factors affecting polymorphism at microsatellite loci in bread wheat [Triticum aestivum (L.) Thell]: effects of mutation processes and physical distance from the centromere. Thuillet, A.-C.; Bataillon, T.; Sourdille, P.; David, J. L. // Theoretical & Applied Genetics;Jan2004, Vol. 108 Issue 2, p368 

    The effects of factors known to influence the level of polymorphism at microsatellite loci were studied using 99 markers and seven lines of bread wheat. Mutational factors as well as indirect selective events shape diversity at these loci. Theory predicts that the selection of favorable alleles...

  • Similarity in Recombination Rate Estimates Highly Correlates with Genetic Differentiation in Humans. Laayouni, Hafid; Montanucci, Ludovica; Sikora, Martin; Melé, Marta; Dall'Olio, Giovanni Marco; Lorente-Galdos, Belén; McGee, Kate M.; Graffelman, Jan; Awadalla, Philip; Bosch, Elena; Comas, David; Navarro, Arcadi; Calafell, Francesc; Casals, Ferran; Bertranpetit, Jaume // PLoS ONE;2011, Vol. 6 Issue 3, p1 

    Recombination varies greatly among species, as illustrated by the poor conservation of the recombination landscape between humans and chimpanzees. Thus, shorter evolutionary time frames are needed to understand the evolution of recombination. Here, we analyze its recent evolution in humans. We...

  • Development of a Linkage Map and Mapping of Phenotypic Polymorphisms in a Free-Living Population of Soay Sheep (Ovis aries). Beraldi, Dario; McRae, Allan F.; Gratten, Jacob; Slate, Jon; Visscher, Peter M.; Pemberton, Josephine M. // Genetics;Jul2006, Vol. 173 Issue 3, p1521 

    An understanding of the determinants of trait variation and the selective forces acting on it in natural populations would give insights into the process of evolution. The combination of long-term studies of individuals living in the wild and better genomic resources for nonmodel organisms makes...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics