TITLE

Evaluation of Mouse Embryonic Stem Cells Plasticity Through Different Differentiation Protocols

AUTHOR(S)
Groza, loan; Emoke, Pall; Elen, Gocza; Olga, Soritau; Zsuzsanna, Lichner; Cenariu, M.; Tomuleasa, C.
PUB. DATE
July 2010
SOURCE
Bulletin of the University of Agricultural Sciences & Veterinary;2010, Vol. 67 Issue 2, p96
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The present study was designed to evaluate the plasticity of mouse embryonic stem cells (ESCs). Cardiac and neural differentiation of the mouse ESCs was initiated by embryoid bodies (EBs) formation in hanging drops and in suspension. After aggregation the were transferred to the suspension culture and then plating on to gelatin-coated tissue culture plates. Retinoic acid was added to culture medium through out the suspension period. Cultures were observed daily withan inverted microscope for the appearance of contracting clusters and neural filaments. At the early,intermediate and terminal stages of differentiation, the choronotropic responses of cardiomyocytes to cardioactive drugs were assessed, and the cardiomyocytes immunostained for citokeratin, titin and betallitubulin. Our data suggest that ESCs can differentiate into functional mature cardiomyocytes in vitro. Furthermore, ESC-cardiomyocytes may provide an ideal model for the study of cardiomyocytic development and may be useful for cell therapy of various cardiac diseases.
ACCESSION #
57208160

 

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