TITLE

Detection of Bovine Herpesvirus Type 1 by PCR Assay

AUTHOR(S)
Aniţă, Dragoş; Aniţă, Adriana; Savuţă, Gheorge
PUB. DATE
July 2010
SOURCE
Bulletin of the University of Agricultural Sciences & Veterinary;2010, Vol. 67 Issue 2, p23
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Bovine herpesvirus I (BHV-l), the causative agent of infectious bovine rhinotracheitis (IBR), is considered to be the most common viral pathogen found in bovine. Bovine Herpesvirus I (BHV- 1) belongs to the genus of Varicellovirus and the family of Herpesviridae which contains three main gB, gC and gD genes. Two polymerase chain reaction (PCR) assays specific for glycoprotein B (gB) and glycoprotein C (gC) gene detection, respectively, were adopted for the detection of bovine herpesvirus-1 (BHV-l) in naturally infected cattle. These two genes are coding for viral envelope. Our study consists in examination of 12 bovine tissue samples, using two protocols work: for gB and gC. We identified one sample found to be positive for presence of viral genome, producing an amplified PCR product of size 173 nt. DNA sample was positive only for gC, for the gB gene amplification the result was negative. Finally, the study revealed the presence of BHV-l in cattle farms and genetic variability of wild-type of bovine herpesvirus type I circulating in cattle populations in Romania.
ACCESSION #
57208146

 

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