Glucocorticoid receptor expression and glucocorticoid therapeutic effect in nasal polyps

Peng Li; Yuan Li; Yong-Qi Li; Qin-Tai Yang; Ge-Hua Zhang
June 2010
Clinical & Investigative Medicine;Jun2010, Vol. 33 Issue 3, pE181
Academic Journal
Purpose: To investigate the expression and quantity of glucocorticoid receptor-α and -β in polyp tissues taken from the patients treated were subsequently treated with topical glucocorticoid (GC). Methods: Eighty patients with nasal polyps were initially enrolled in the study. All polyp specimens were obtained prior to treatment. Patients then received daily topical GC spray treatment for one month. Polyp specimens were tested for glucocorticoid receptor (GR) GR-α and GR-β mRNA expression using fluorescent quantitative-reverse transcription-polymerase chain reaction (FQ-RT-PCR). Thirty healthy nasal mucosa tissue samples were tested at the same time. Results: Forty patients finished the study and were divided into two groups: GC-sensitive (n=26) and GC-insensitive (n=14), according to treatment results. GR-β mRNA expression in the nasal polyp tissues of the GC-insensitive group (5.72±0.58×10² copies/μg) was higher than that in the GC-sensitive group (4.82±0.28×10² copies/μg, P<0.05) and in the normal nasal mucosa group (4.44±0.35×10² copies/μg, P<0.01). There was also a difference in the relative expression of GR-α and GR-β between the GC-sensitive group (GR-α/GR-β= 829.42±67.36) and the GC-insensitive group (535.7±89) (P<0.01). Conclusion: GR-β mRNA was highly expressed in patients with nasal polyps. Down-regulation of GR-α mRNA suggests the existence of glucocorticoid insensitivity. Expression of GR-β may plays an important role in the evaluation of the glucocorticoid therapeutic effect in patients with nasal polyps.


Related Articles

  • Synergistic induction of thymic stromal lymphopoietin by tumor necrosis factor alpha and Th2 cytokine in nasal polyp fibroblasts. Nonaka, Manabu; Fukumoto, Akira; Ogihara, Nozomu; Sakanushi, Atsuko; Pawankar, Ruby; Yagi, Toshiaki // American Journal of Rhinology & Allergy;Jan2010, Vol. 24 Issue 1, p14 

    Background: Thymic stromal lymphopoietin (TSLP) is elevated in airway inflammatory diseases such as asthma and triggers dendritic cell-mediated activation of Th2 inflammatory responses. Although allergic chronic sinusitis is a Th2 inflammatory disease of the upper airway, the mechanism...

  • Corneal Epithelium Expresses a Variant of P2X7 Receptor in Health and Disease. Mankus, Courtney; Rich, Celeste; Minns, Martin; Trinkaus-Randall, Vickery // PLoS ONE;2011, Vol. 6 Issue 12, p1 

    Improper wound repair of the corneal epithelium can alter refraction of light resulting in impaired vision. We have shown that ATP is released after injury, activates purinergic receptor signaling pathways and plays a major role in wound closure. In many cells or tissues, ATP activates P2X7...

  • Impact of mitomycin C on the mRNA expression signatures of immunological biomarkers in eosinophilic nasal polyposis. de Castro, Mirian Cabral Moreira; Rocha-Silva, Fabiana; Gomes, Luciana Inácia; Zauli, Danielle Alves Gomes; Mourão, Marina de Moraes; de Castro, Mariana Moreira; Guimarães, Roberto Eustáquio Santos; Teixeira-Carvalho, Andrea; Martins-Filho, Olindo Assis // American Journal of Rhinology & Allergy;Jan/Feb2013, Vol. 27 Issue 1, pe32 

    BACKGROUND: The topical application of mitomycin C has been evaluated as a complementary therapy for eosinophilic nasal polyposis (ENP). However, the mechanism underlying the additional benefits of mitomycin C for the control of eosinophilic inflammation and prevention of posttherapeutic relapse...

  • Antigen selection in IgE antibodies from individuals with chronic rhinosinusitis with nasal polyps. Pratt, Eleanor; Collins, Andrew M.; Sewell, William A.; Harvey, Richard J. // American Journal of Rhinology & Allergy;Nov/Dec2010, Vol. 24 Issue 6, p416 

    Background: It is generally assumed that all immunoglobulin isotopes develop under antigen selection pressure, leading to dramatic increases in antigen-binding affinity. As activated B cells proliferate, somatic mutations accumulate in the regions of the immunoglobulin gene associated with...

  • Different expressions of erbB1/2 and tight junction proteins in hypertrophic inferior turbinates and nasal polyps. Nguyen, Khac-Hung; Suzuki, Hideaki; Wakasugi, Tetsuro; Hohchi, Nobusuke; Hashida, Koichi; Ohbuchi, Toyoaki // European Archives of Oto-Rhino-Laryngology;Mar2013, Vol. 270 Issue 3, p945 

    Both inferior turbinate hypertrophy and nasal polyp formation entail the enlargement of the nasal mucosa caused by rhinosinusitis, but their macro/microscopic and clinical findings differ markedly. This study aimed at investigating differences in the expressions of erbB1/2 and the tight junction...

  • Effects of budesonide on the expression of the glucocorticoid receptor-α in nasal polyp epithelial cells. Wang, De-Sheng; Liu, Qin-Song; Lai, Hai-Chun // American Journal of Rhinology & Allergy;Mar/Apr2013, Vol. 27 Issue 2, p123 

    Background: This study explores effects of budesonide on the proliferation of nasal polyp epithelial cells and expression of the glucocorticoid receptor (GR) alpha in nasal polyp epithelial cells. Methods: Primary cultured, purified, and identified the epithelial cells collected from nasal...

  • Serum miR-122 as a Biomarker of Necroinflammation in Patients With Chronic Hepatitis C Virus Infection. Bihrer, Verena; Friedrich-Rust, Mireen; Kronenberger, Bernd; Forestier, Nicole; Haupenthal, Jörg; Ying Shi; Peveling-Oberhag, Jan; Radeke, Heinfried H.; Sarrazin, Christoph; Herrmann, Eva; Zeuzem, Stefan; Waidmann, Oliver; Piiper, Albrecht // American Journal of Gastroenterology;Sep2011, Vol. 106 Issue 9, p1663 

    OBJECTIVES:The liver contains large amounts of microRNA-122 (miR-122), whereas other tissues contain only marginal amounts of this miRNA. MicroRNAs have also been found to circulate in the blood in a cell-free form; their potential as readily accessible disease markers is currently evaluated....

  • Increased expression of intelectin-1 in nasal polyps. Park, Il-Ho; Park, Se-Jin; Cho, Jung-Sun; Moon, You-Mi; Kim, Tae Hoon; Lee, Sang Hag; Lee, Heung-Man // American Journal of Rhinology & Allergy;Jul/Aug2012, Vol. 26 Issue 4, p274 

    Background: Intelectin-1 is a new type of Ca2+-dependant soluble lectin in humans that has affinity for galactofuranose in carbohydrate chains of bacterial cell walls, indicating that intelectin-1 may play a role in immune defense against bacteria. The purpose of the current study was to...

  • Airborne Fungi Induce Nasal Polyp Epithelial Cell Activation and Toll-Like Receptor Expression. Seung-Heon Shin; Young-Ho Lee // International Archives of Allergy & Immunology;2010, Vol. 153 Issue 1, p46 

    Background: The nasal epithelium is the first barrier encountered by airborne allergens and is an active participant in airway inflammation. Fungi have been increasingly recognized as important pathogens in sinusitis and airway diseases. The aim of the study was to evaluate fungal protease...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics