Effects of Autologous Platelet-Rich Plasma on Cell Viability and Collagen Synthesis in Injured Human Anterior Cruciate Ligament

Fallouh, Louay; Nakagawa, Koichi; Sasho, Takahisa; Arai, Momoko; Kitahara, Sota; Wada, Yuichi; Moriya, Hideshige; Takahashi, Kazuhisa
December 2010
Journal of Bone & Joint Surgery, American Volume;12/15/2010, Vol. 92-A Issue 18, p2909
Academic Journal
Background: Platelet-rich plasma is a fraction of plasma in which platelets are concentrated. It is reported to represent a source of multiple growth factors that promote tissue repair. In anticipation of the eventual testing of platelet-rich plasma in anterior cruciate ligament (ACL)-deficient patients, we examined the effect of autologous platelet-rich plasma on human ACL cell function in vitro. Methods: Fresh blood and ACL remnants were obtained from four patients who underwent ACL reconstruction surgery. Platelet-poor plasma and platelet-rich plasma were prepared from the blood samples. The concentrations of various growth factors in each preparation were tested with use of enzyme-linked immunosorbent assays. Isolated ACL cells were cultured in the presence of 5% fetal bovine serum, 5% platelet-poor clot releasate, 5% platelet-rich clot releasate, or 10% platelet-rich clot releasate. Platelet-rich plasma and platelet-poor plasma releasates were applied to the ACL cells from the same patient autologously. Cell viability and collagen synthesis in each group were analyzed, and semiquantitative gene-expression assays for type-I and Ill collagen were also performed. Results: The concentrations of the main growth factors (transforming growth factor-beta, platelet-derived growth factor, epidermal growth factor, and vascular endothelial growth factor) were much higher in platelet-rich clot releasate than in platelet-poor clot releasate. In vitro treatment of ACL cells with platelet-rich clot releasate resulted in a significant increase in cell number compared with platelet-poor clot releasate. Total collagen production by the platelet- rich clot releasate-treated cells was significantly higher than that of the platelet-poor clot releasate-treated cells only because of enhanced cell proliferation. There was no significant effect of platelet-rich clot releasate treatment on gene expression for type-I collagen, but expression of type-III collagen was significantly enhanced by the treatment with platelet- rich clot releasate. Conclusions: These results suggest that autologous platelet-rich plasma can enhance ACL cell viability and function in vitro.


Related Articles

  • Concentration of PDGF-AB, BB and TGF-ß1 as Valuable Human Serum Parameters in Adipose-derived Stem Cell Proliferation. Josh, Fonny; Tobita, Morikuni; Tanaka, Rica; Orbay, Hakan; Ogata, Kasumi; Suzuki, Koji; Hyakusoku, Hiko; Mizuno, Hiroshi // Journal of Nippon Medical School;2013, Vol. 80 Issue 2, p140 

    Introduction: Human serum (HS) has attributes similar to fetal bovine serum (FBS) in the proliferation and differentiation of human adipose-derived stem cells (hASCs) when compared in vitro. The purpose of this study was to determine what types of HS, with respect to the concentrations of...

  • Soluble ICAM-1 in breast cancer: clinical significance and biological implications. Köstler, Wolfgang J.; Tomek, Sandra; Brodowicz, Thomas; Budinsky, Alexandra C.; Flamm, Maria; Hejna, Michael; Krainer, Michael; Wiltschke, Christoph; Zielinski, Christoph C. // Cancer Immunology, Immunotherapy;Nov2001, Vol. 50 Issue 9, p483 

    Objectives: In previous experiments, we demonstrated a decreased expression of intercellular adhesion molecule 1 (ICAM-1) on both tumour cells and antigen-presenting cells derived from patients with breast cancer, resulting in an abrogation of antigen presentation and tumour cell lysis....

  • The effect of platelet-derived growth factors on knee stability after anterior cruciate ligament reconstruction: a prospective randomized clinical study. Vogrin, Matjaž; Rupreht, Mitja; Crnjac, Anton; Dinevski, Dejan; Krajnc, Zmago; Rečnik, Gregor // Wiener Klinische Wochenschrift;May2010 Supplement, Vol. 122, p91 

    BACKGROUND: Arthroscopic reconstruction is a standard surgical procedure in cases of symptomatic knee instability due to rupture of the anterior cruciate ligament. Bone-tendon-bone and hamstring tendon grafts are both in use for anterior cruciate ligament reconstruction. There are no significant...

  • New options for healing torn ACLs. Stanton, Terry // AAOS Now;Jun2010, Vol. 4 Issue 6, p18 

    The article discusses the surgical options for repairing a torn anterior cruciate ligament (ACL). It states that collagen-fibrin scaffolds could help repair a torn ACL due to the copolymer that it forms. It notes that in vivo testing of platelet-rich plasma (PRP) and collagen is continuing to...

  • Antibody microarray profiling of osteosarcoma cell serum for identifying potential biomarkers. ZI-QIANG ZHU; JIN-SHAN TANG; DUAN GANG; MING-XING WANG; JIAN-QIANG WANG; ZHOU LEI; ZHOU FENG; MING-LIANG FANG; LIN YAN // Molecular Medicine Reports;2015, Vol. 12 Issue 1, p1157 

    The aim of the present study was to identify biomarkers in osteosarcoma (OS) cell serum by antibody microarray profiling, which may be used for OS diagnosis and therapy. An antibody microarray was used to detect the expression levels of cytokines in serum samples from 20 patients with OS and 20...

  • Selectins and Platelet-Derived Growth Factor (PDGF) in Schistosomiasis-Associated Pulmonary Hypertension. Lapa, Monica; Acencio, Milena; Farias, Alberto; Teixeira, Lisete; Fernandes, Caio; Jardim, Carlos; Terra-Filho, Mario // Lung;Dec2014, Vol. 192 Issue 6, p981 

    Purpose: The aim of this study was to evaluate the expression profiles of the relevant selectins and PDGF in schistosomiasis-associated pulmonary hypertension. Methodology: Patients with three distinct clinical profiles were enrolled in the study: IPAH( n = 11), schistosomiasis-associated PH...

  • Serum TNF-alpha levels: potential use to indicate osteoarthritis progression in a mechanically induced model. Aktas, Erdem; Sener, Ertugrul; Zengin, Ozlem; Gocun, Pinar; Deveci, Mehmet // European Journal of Orthopaedic Surgery & Traumatology;Feb2012, Vol. 22 Issue 2, p119 

    To investigate plasma tumor necrosis factor-alpha (TNF-α) levels as an indicator to reflect the magnitude of the destructive inflammatory phase and articular cartilage damage after a knee trauma. Eighteen mature Wistar Albino male rats were divided into two groups equal in number. Nine...

  • Pre-injury serum biomarkers associated with risk of subsequent ACL rupture. Brockenbrough, Gina // Orthopedics Today;Oct2012, Vol. 32 Issue 10, p32 

    The article discusses a study by researchers from Texas Technical University Health Sciences Center, U.S. Military Academy and Keller Army Community Hospital, hypothesizing the association of pre-injury serum biomarkers and risk of anterior cruciate ligamnt (ACL). It performed a multivariate...

  • Comparison of epitheliotrophic factors in autologous serum eyedrops from sera of chronic renal failure patients vs. normal controls. Kang, Na; Lee, Shina; Jun, Roo // Graefe's Archive of Clinical & Experimental Ophthalmology;Oct2015, Vol. 253 Issue 10, p1705 

    Purpose: To investigate the composition and biological activity of serum epitheliotrophic factors from chronic renal failure (CRF) patients vs. healthy controls. Methods: Twenty, 50 and 100 % autologous serum eyedrops (ASEs) were prepared from 16 CRF patients and 16 normal subjects. Serum...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics