TITLE

Tomographic imaging of cryogenic biological specimens with the X-ray microscope at BESSY I

AUTHOR(S)
Weiß, D.; Schneider, G.; Niemann, B.; Guttmann, P.; Rudolph, D.; Schmahl, G.
PUB. DATE
May 2000
SOURCE
AIP Conference Proceedings;2000, Vol. 507 Issue 1, p123
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Soft X-ray microscopy employs the natural absorption contrast between water and protein in the 2.34 - 4.38 nm wavelength region with a resolution down to 30 nm. The large depth of focus of the Fresnel zone plates used as X-ray objectives permits tomographic reconstruction based on the microscopic images. High-resolution images require a high specimen radiation dose, and a large number of images taken at different viewing angles is needed for tomographic reconstruction. Therefore, cryo microscopy is necessary to preserve the structural integrity of hydrated biological specimens during image acquisition. The cryo transmission X-ray microscope at the electron storage ring BESSY I (Berlin) was used to obtain a tilt series of images of the frozen-hydrated green alga Chlamydomonas reinhardtii. The living specimens were inserted into borosilicate capillaries, then rapidly cooled by plunging into liquid nitrogen. The capillary specimen holders allow image acquisition over the full angular range of 180°. The reconstruction shows details inside the alga down to 60 nm size and conveys a detailed impression of the specimen structure. This technique is expected to be applicable to a wide range of biological specimens, such as the cell nucleus, It offers the possibility of imaging the three-dimensional structure of hydrated biological specimens close to their natural living state.
ACCESSION #
5664450

 

Related Articles

  • X-ray microscopy study of track membranes and biological objects. Artioukov, I. A.; Asadchikov, V. E.; Gulimova, V. I.; Levashov, V. E.; Mchedlishvili, B. V.; Kurohtin, A. N.; Postnov, A. A.; Popov, A. V.; Saveliev, S. V.; Struk, I. I.; Vilensky, A. I.; Vinogradov, A. V.; Zagorsky, D. L. // AIP Conference Proceedings;2000, Vol. 507 Issue 1, p135 

    The development of two types of X-ray microscopy applying to the organic objects investigation (biological samples and polymer matrix) is reported. Polymer track membranes were investigated using Schwarzchild X-ray microscope with 20 nm wavelength. Pore diameters down to 0.2 μm were clearly...

  • The use of absorption difference at distant wavelengths for imaging elements in mammalian cells. Ito, Atsushi; Endo, Tetsuya; Yamamoto, Yoshimasa; Shinohara, Kunio // AIP Conference Proceedings;2000, Vol. 507 Issue 1, p149 

    Intracellular mapping of elements was performed for Cd or Hg loaded mammalian cells. X-ray contact microscopy with an electronic zooming tube were used for cell imaging at the resolution of 0.5 μm. The mapping was obtained by the subtraction between images taken at two different wavelengths....

  • State of art micro-CT. Sassov, A. // AIP Conference Proceedings;2000, Vol. 507 Issue 1, p515 

    By using high technology an X-ray microtomograph (micro-CT) was created as a simply usable, desktop instrument. The micro-CT is a laboratory system for non-destructive three-dimensional microscopy, giving true spatial resolution over a million times more detailed than the medical CT-scanners.

  • High-resolution X-ray imaging and tomography at the ESRF beamline ID 22. Weitkamp, T.; Drakopoulos, M.; Leitenberger, W.; Raven, C.; Schroer, C.; Simionovici, A.; Snigireva, I.; Snigirev, A. // AIP Conference Proceedings;2000, Vol. 507 Issue 1, p424 

    At the ESRF micro-fluorescence, imaging and diffraction (μ-FID) beamline ID 22, a microimaging and tomography setup has been operational for several months. The coherence properties of the high-energy X-ray undulator beam at ID 22 make the setup especially suited for phase-contrast tomography...

  • Electron and X-ray microscopy. Pulizzi, Fabio // Nature Materials;Apr2009, Vol. 8 Issue 4, p259 

    The article discusses various reports published within the issue including one on the use of electron tomography and another on the X-ray microscopy as another breakthrough in electron microscopy.

  • Phase-contrast microtomography with polychromatic sealed source. Sassov, A.; Van Dyck, D. // AIP Conference Proceedings;2000, Vol. 507 Issue 1, p521 

    Conventional X-ray microradiography and microtomography are based on X-ray attenuation inside an object. For light objects (in the terms of X-ray absorption) much better way would be to use phase contrast, rather than attenuation contrast. Recently it has been shown that one can obtain phase by...

  • X-ray digital microlaminography for BGA and flip-chip inspection. Sassov, Alexander; Luypaert, Filip // AIP Conference Proceedings;2000, Vol. 507 Issue 1, p239 

    X-ray laminography allows getting local depth information from big flat objects like PCBs and electronic assemblies, which cannot be reconstructed by tomographical approach. According to the needs in high-resolution inspection for electronic and micromechanic industries an X-ray...

  • Potential application of vanadium probes for biological x-ray microscopy. Moronne, M. M.; Hamamoto, D. J.; Meigs, G.; Johnson, L. E.; Denbeaux, G. P.; Meyer-Ilse, W. // AIP Conference Proceedings;2000, Vol. 507 Issue 1, p184 

    Soft x-ray microscopy is now routinely capable of imaging biological specimens with resolutions that are five times better than the best visible light microscopes (≤ 50 nm). However, for biological labeling the only options developed for x-ray microscopy have been silver enhanced gold...

  • Soft x-ray scanning microtomography with submicrometer resolution. McNulty, I.; Haddad, W.S.; Trebes, J.E.; Anderson, E.H. // Review of Scientific Instruments;Feb1995, Vol. 66 Issue 2, p1431 

    Discusses the use of scanning soft x-ray microtomography to obtain high-resolution three-dimensional images of a microfabricated test object. Description of the high-resolution scanning transmission x-ray microscope; Addition of a precision specimen rotation stage to the instrument; Data...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics