Application of X-ray microscopy in food science investigation of high pressure affected bacterial spores

Mo¨nch, Susanne; Heinz, Volker; Guttmann, Peter; Knorr, Dietrich
May 2000
AIP Conference Proceedings;2000, Vol. 507 Issue 1, p225
Academic Journal
Using the Göttingen transmission X-ray microscope at BESSY the effect of different pressure and temperature levels during the high hydrostatic pressure (HP) treatment was investigated. At 150 MPa and temperatures up to 50°C the triggering of germination was observed by standard microbiological methods with Bacillus subtilis spores, Increasing the temperature to 70°C at the same pressure level killed the spores without any indication of germination, By X-ray microscopy images it could be shown that the typical disintegration of the protoplast is inhibited. This suggests that the enzymic reaction pathway is possibly affected under specific pressure temperature conditions.


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