Use of the high-energy X-ray microprobe at the advanced photon source to investigate the interactions between metals and bacteria

Kemner, K. M.; Lai, B.; Maser, J.; Schneegurt, M. A.; Cai, Z.; Ilinski, P. P.; Kulpa, C. F.; Legnini, D. G.; Nealson, K. H.; Pratt, S. T.; Rodrigues, W.; Tischler, M. Lee; Yun, W.
May 2000
AIP Conference Proceedings;2000, Vol. 507 Issue 1, p319
Academic Journal
Understanding the fate of heavy-metal contaminants in the environment is of fundamental importance in the development and evaluation of effective remediation and sequestration strategies. Among the factors influencing the transport of these contaminants are their chemical speciation and the chemical and physical attributes of the surrounding medium. Bacteria and the extracellular material associated with them are thought to play a key role in determining a contaminant's speciation and thus its mobility in the environment. In addition, the microenvironment at and adjacent to actively metabolizing cell surfaces can be significantly different from the bulk environment. Thus, the spatial distribution and chemical speciation of contaminants and elements that are key to biological processes must be characterized at micron and submicron resolution in order to understand the microscopic physical, geological, chemical, and biological interfaces that determine a contaminant's macroscopic fate. Hard X-ray microimaging is a powerful technique for the element-specific investigation of complex environmental samples at the needed micron and submicron resolution. An important advantage of this technique results from the large penetration depth of hard X-rays in water. This advantage minimizes the requirements for sample preparation and allows the detailed study of hydrated samples. This paper presents results of studies of the spatial distribution of naturally occurring metals and a heavy-metal contaminant (Cr) in and near hydrated bacteria (Pseudomonas fluorescens) in the early stages of biofilm development, performed at the Advanced Photon Source Sector 2 X-ray microscopy beamline.


Related Articles

  • Silica deposition in Demosponges: spiculogenesis in Crambe crambe. Uriz, María J.; Turon, Xavier; Becerro, Mikel A. // Cell & Tissue Research;Aug2000, Vol. 301 Issue 2, p299 

    Transmission electron-microscopy images coupled with dispersive X-ray analysis of the species Crambe crambe have provided information on the process of silica deposition in Demosponges. Sclerocytes (megasclerocytes) lie close to spicules or surround them at different stages of growth by means of...

  • A brief history of 50 years of ICXOM. Janssens, K. // AIP Conference Proceedings;4/6/2010, Vol. 1221 Issue 1, p1 

    The ICXOM series of meetings began in 1956 and for over 50 years have been conferences devoted to the topic of “X-ray microanalysis,” both for those interested in developing instrumentation and methods of analysis and for scientists mainly interested in using X-ray micro-analysers...

  • Dust-particle migration around flotation tailings ponds: pine needles as passive samplers. Teper, Ewa // Environmental Monitoring & Assessment;Jul2009, Vol. 154 Issue 1-4, p383 

    The potential use of dust particles trapped on Scots pine needles for tracking dust migration around flotation tailings ponds in the Silesian–Krakowian ore district, Southern Poland was tested. 1-, 2- and 3-years old needles were studied using a scanning electron microscope and energy...

  • Characterisation by X-ray microanalysis of metal granules in the mucus trails of Littorina littorea (Gastropoda) along a putative pollution gradient. Reboreda, R.; Davies, Mark S. // Ecotoxicology;Jul2006, Vol. 15 Issue 5, p403 

    Metal-containing granules in the mucus trails of the marine gastropod Littorina littorea from nine sites in north-east England were analysed for elemental composition by X-ray microanalysis and characterised relative to a putative gradient of pollution. Overall granule density varied...

  • Analytical Characterisation of Solid- and Solution-Phase Chromium Species at Copr-Contaminated Sites. Thomas, R. P.; Hillier, S. J.; Roe, M. J.; Geelhoed, J. S.; Graham, M. C.; Paterson, E.; Farmer, J. G. // Environmental Geochemistry & Health;Sep2001, Vol. 23 Issue 3, p195 

    The article presents analytical characterisation of solid and solution-phase chromium species at chromite ore processing residue (COPR)-contaminated sites. The solid phases present in COPR from three of the sites investigated were identified and quantified using X-ray powder diffractometry and...

  • The SEM Studies and X-Ray Microanalysis -- the Key for Understanding the Origin and Fate of Airborne Particulates. Migaszewski, Z. M.; Galuszka, A.; Starnawska, E.; Paslawski, P. // Conference Proceedings: IX International Symposium on Trace Elem;2006, p143 

    The occurrence of airborne particulates in the environment is a question that involves many implications in the environmental and biological sciences, toxicology and other related disciplines. Here we show that the mineralogical studies are the best method for determining the origin and pathways...

  • New Data on the Ceratophyllum demersum L. as an Environmental Pollution Bioindicator. llyashenko, N. V.; Petrova, M. B.; Pavlova, N. V.; Kharitonova, E. A.; Kurbatova, L. A. // Annual Review & Research in Biology;2014, Vol. 4 Issue 2, p366 

    Aims: To perform an experimental analyses of the chemical composition and anatomic structure of polluted higher aquatic plants with the aid of combined physical methods of characterization by infrared spectroscopy, scanning electron microscopy and X-ray microanalysis to validate their use for...

  • Cellular dosimetry of strontium-90 using the Monte Carlo code MCNPX, detection and X-ray microanalysis. Hocinela, N.; Farlay, D.; Bertho, J.-M.; Boivin, G.; Desbrée, A.; Franck, D.; Agarande, M. // Radioprotection (Cambridge University Press);2014, Vol. 49 Issue 2, p101 

    The aim of this study is to provide data that increase knowledge about the possible health effects of exposure to 90Sr contaminant in drinking water. 90Sr detection in the mouse bone model plays an important complementary role with computational dosimetry in these investigations. S-value...

  • Quantitative energy-dispersive electron probe X-ray microanalysis for single-particle analysis and its application for characterizing atmospheric aerosol particles. MASKEY, SHILA; RO, CHUL-UN // Pramana: Journal of Physics;Feb2011, Vol. 76 Issue 2, p281 

    n energy-dispersive electron probe X-ray microanalysis (ED-EPMA) technique using an energy-dispersive X-ray detector with an ultra-thin window, designated as low- Z particle EPMA, has been developed. The low- Z particle EPMA allows the quantitative determination of concentrations of low- Z...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics