Soft X-Ray Contact Microscopy using the Asterix Laser Source

Stead, A. D.; Ford, T. W.; Marranca, A.; Batani, D.; Botto, C.; Masini, A.; Bortolotto, F.; Eidmann, K.
May 2000
AIP Conference Proceedings;2000, Vol. 507 Issue 1, p731
Academic Journal
The use of a high-powered laser (Asterix) has permitted comparisons of the soft x-ray emission from various target materials and the quality of images of hydrated biological specimens produced by soft x-ray contact microscopy to be performed. Although targets such as Zr produced lower conversion efficiencies the soft x-ray flux was still sufficient to produce good quality images. However, with gold foil targets the proportion of hard x-rays was high and detracted from image quality. Images of Chlamydomonas showed the x-ray absorbing spheres reported previously, and in yeast the changes in carbon density within the cytoplasm have been related to the cell cycle. Using spematozoa the paired microtubules of the flagella were imaged thus confirming a resolution for the technique of about 40-50nm.


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