TITLE

Efficient Gene Expression in Skin Wound Sites Following Local Plasmid Injection

AUTHOR(S)
Meuli, Martin; Liu, Yong; Liggitt, Denny; Kashani-Sabet, Mohammed; Knauer, Sharon; Meuli-Simmen, Claudia; Harrison, Michael R.; Adzick, N. Scott; Heath, Timothy D.; Debs, Robert J.
PUB. DATE
January 2001
SOURCE
Journal of Investigative Dermatology;Jan2001, Vol. 116 Issue 1, p131
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
SummaryTransfection of the skin by local gene delivery, as well as widespread transfection of systemic tissues following intravenous injection of cationic liposome/DNA complexes have been reported. Here, we show that surgically wounded mouse skin can be transfected either by local injection of DNA alone or by intravenous injection of optimized cationic liposome/DNA complexes; however, direct cutaneous injection produces much higher levels of gene expression in the skin, which is targeted to dermal and subdermal layers. High levels of chloramphenicol acetyltransferase activity were present from 3 h to 2 wk following direct injection of a gene expression plasmid into wounded skin and were maintained at detectable levels up to 8 wk after injection. Expres- sion of transferred chloramphenicol acetyltransferase as well as β-GAL genes was localized to fibroblasts, macrophages, and adipocytes as determined by histochemistry and immunohistochemistry. Further- more, local injection of a human granulocyte- colony-stimulating factor gene expression plasmid produced high levels of the biologically relevant human granulocyte-colony-stimulating factor protein in wounded mouse skin. This efficient and simple method of site-specific gene transfer into wounds may lead to the development of cutaneous gene therapy directed against disorders of abnormal cutaneous wound healing.
ACCESSION #
5661548

 

Related Articles

  • Effects of Plasmid DNA Injection on Cyclophosphamide-Accelerated Diabetes in NOD Mice. Filippova, Maria; Liu, Jingxue; Escher, Alan // DNA & Cell Biology;Mar2001, Vol. 20 Issue 3, p175 

    Type 1 diabetes results in most cases from the destruction of insulin-secreting beta cells by the immune system. Several immunization methods based on administration of autoantigenic polypeptides such as insulin and glutamic acid decarboxylase (GAD) have been used to prevent autoimmune diabetes...

  • Immunization with a plasmid expressing pneumococcal surface protein A (PspA) can elicit protection against fatal infection with Streptococcus pneumoniae. McDaniel, L S; Loechel, F; Benedict, C; Greenway, T; Briles, D E; Conry, R M; Curiel, D T // Gene Therapy;Apr97, Vol. 4 Issue 4, p375 

    Pneumococcal surface protein A (PspA) is a protection-eliciting protein of Streptococcus pneumoniae. We observed that immunization of BALB/c mice with a plasmid expressing PspA significantly protected the mice from lethal challenge with S. pneumoniae when compared to control mice that received...

  • The φC31 Integrase System for Gene Therapy. Calos, Michele P. // Current Gene Therapy;Dec2006, Vol. 6 Issue 6, p633 

    The φC31 integrase system represents a novel technology that opens up new possibilities for gene therapy. The φC31 integrase can integrate introduced plasmid DNA into preferred locations in unmodified mammalian genomes, resulting in robust, long-term expression of the integrated...

  • Visualization of the transgene distribution according to the administration route allows prediction of the transfection efficacy and validation of the results obtained. Delepine, P.; Montier, T.; Guillaume, C.; Vaysse, L.; Le Pape, A.; Ferec, C. // Gene Therapy;6/1/2002, Vol. 9 Issue 11, p736 

    Gene transfer to the lung can be achieved via a systemic, that targets the endothelium, or local, that targets the epithelium, delivery route. In the present study, we followed the distribution of a plasmid after transfection using some of our phosphonolipids, which have previously shown their...

  • Researchers Find Key to Regulation of Skin Wound Healing.  // O&P News;Aug2015, Vol. 24 Issue 9, p30 

    The article discusses a 2015 study by Ning Xu Landen and colleagues and published in the "Journal of Clinical Investigation" that revealed the potential of using microRNA to regulate skin would healing. The study looked for changes in miRNA expression during the healing process and explored the...

  • Noninvasive, Repetitive, Quantitative Measurement of Gene Expression from a Bicistronic Message by Positron Emission Tomography, Following Gene Transfer with Adenovirus Liang, Qianwa; Gotts, Jeff; Satyamurthy, Nagichettiar; Barrio, Jorge; Phelps, Michael E.; Gambhir, Sanjiv S.; Herschman, Harvey R. // Molecular Therapy;Jul2002, Vol. 6 Issue 1, p73 

    Gene therapy protocols are hampered by the inability to monitor the location, magnitude, and duration of ectopic gene expression following DNA delivery. Consequently, it is difficult to establish quantitative correlations and/or causal relationships between therapeutic gene expression and...

  • In utero gene therapy: The case against. Billings, Paul R. // Nature Medicine;Mar1999, Vol. 5 Issue 3, p255 

    The prospect of in utero gene therapy presents both tremendous opportunities and real concerns. With the possibility of first clinical trials as little as two years away, now is the time to discuss the merits of this new technology. In this spirit, we present arguments for and against the...

  • Regulated gene expression systems. Clackson, T // Gene Therapy;Jan2000, Vol. 7 Issue 2, p120 

    Most gene therapy research to date has focused on solving the delivery problem — getting genes efficiently and stably into target cells and tissues. Those working on systems for regulating the expression of genes once delivered have often been accused of trying to run before they can...

  • The many ways to make an iPS cell. Lowry, William E; Plath, Kathrin // Nature Biotechnology;Nov2008, Vol. 26 Issue 11, p1246 

    The article discusses three studies that suggest alternative methods of transgene delivery and the use of small molecules for induced pluripotent stem cells (iPS). These new methodologies are intended to circumvent the disruption of endogenous gene expression which increases the risk of...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics