Total phenolics and individual phenolic acids vary with light environment in Lindera benzoin

Ingersoll, Christine M.; Niesenbaum, Richard A.; Weigle, Caitlin E.; Lehman, Julia H.
November 2010
Botany;Nov2010, Vol. 88 Issue 11, p1007
Academic Journal
The understory shrub Lindera benzoin L. experiences lower rates of herbivory in sun environments than in shade environments. The production of secondary metabolites (e.g., phenolic compounds with known plant defense properties) is one likely contributor to these observed differences in herbivory. This work determined the total phenolic content as well as the concentrations of several individual phenolic acids in L. benzoin leaves found in sun and shade habitats. Total phenolic concentrations were determined to be higher in leaves from sun plants than in those from shade plants (47.5 ±2.4 vs. 28.6 ±1.3 gallic acid equivalents, respectively). High-performance liquid chromatography with diode array detection was used to separate and quantify several individual phenolic acids, and specific compounds were identified based on their retention times and ultraviolet spectra. The concentrations of vanillic, chlorogenic, p-coumaric, and ferulic acids were shown to be statistically higher in leaves from sun plants than in those from shade plants (P < 0.05), whereas 2,5-dihydroxybenzoic acid and caffeic acid were not significantly different in L. benzoin leaves from sun versus shade habitats. La plante de sous-bois, Lindera benzoin L., subit un plus faible taux d'herbivorie dans les environnements ensoleillés qu'ombragés. Il semble que la production de métabolites secondaires (ex., composés phénoliques, bien connus pour leurs propriétés de défense de la plante) contribue à ces différences observées d'herbivorie. Les auteurs ont déterminé la teneur totale en phénols ainsi que la teneur de plusieurs acides phénoliques individuels chez des feuilles du L. benzoin provenant d'habitats ensoleillés et ombragés. On constate que les teneurs totales en phénols de plantes poussant au soleil dépassent celles des plantes ombragées (47,5 ±2,4 vs. 28,6 ±1,3 équivalent d'acide gallique, respectivement). Les auteurs ont utilisé la chromatographie liquide à haute performance munie d'un système de détection à diode, pour séparer et quantifier les acides phénoliques individuels; ils ont identifié les composés spécifiques sur la base de leurs temps de rétention et leur spectre UV. On démontre ainsi que les teneurs en acides vanillique, chlorogénique, p-coumarique, et férulique diffèrent statistiquement dans les feuilles des plantes ensoleillées par rapport à celles venant à l'ombre (P < 0,05), alors que les acides 2,5-dihydroxybenzoïque et cafféïque ne montrent pas de différence significative entre les feuilles provenant d'habitats ensoleillés et ombragés.


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