Two BTB proteins function redundantly as negative regulators of defense against pathogens in Arabidopsis

Qu, Na; Gan, Wenjian; Bi, Dongling; Xia, Shitou; Li, Xin; Zhang, Yuelin
November 2010
Botany;Nov2010, Vol. 88 Issue 11, p953
Academic Journal
The BTB domain is a protein-protein interaction motif found throughout eukaryotes. Here we report the identification of two closely related BTB domain-containing proteins, POZ/BTB CONTAINING-PROTEIN 1 (POB1) and POB2, as negative regulators of defense against pathogens. In yeast two-hybrid assays, POB1 and POB2 dimerize through their BTB domains. The pob1-1 pob2-1 double mutant plants exhibited enhanced resistance against the fungal pathogen Botrytis cinerea and the oomycete pathogen Hyaloperonospora arabidopsidis Noco2. Double knockout, but not single mutants of pob1-1 and pob2-1, displayed enhanced sensitivity to growth inhibition by jasmonic acid (JA). In addition, expression of the JA-responsive defensin gene PDF1.2 is enhanced in the pob1-1 pob2-1 double mutant. Our data suggest that POB1 and POB2 function redundantly as negative regulators of JA-mediated pathogen resistance responses. Le domaine BTB constitue un patron d'interaction protéine-protéine que l'on retrouve chez l'ensemble des eucaryotes. Les auteurs font état de l'identification de deux protéines étroitement liées du domaine contenant les BTB POZ/BTB CONTAINING-PROTEIN 1 (POB1) et POB2, comme régulateurs négatifs de la défense contre des organismes pathogènes. Dans des tests à double hybridation chez la levure, les POB1 et POB2 se dimérisent sur l'ensemble de leurs domaines BTB. Les plantes avec le double mutant pob1-1 pob2-1 montrent une résistance accrue contre le champignon pathogène Botrytis cinerea et l'oomycète pathogène Hyaloperonospora arabidopsidis Noco2. La double invalidation, mais non pas les simples mutants de pob1-1 et pob2-1, montrent une sensibilité accrue à l'inhibition de la croissance par l'acide jasmonique (JA). De plus, l'expression du gène de la défensine PDF1.2 réagissant à la JA se voit accrue chez le double mutant pob1-1 pob2-1. Les résultats suggèrent que le POB1 et le POB2 fonctionnent de façon redondante comme régulateurs négatifs des réactions de défense aux pathogènes via la JA.


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