Molecular Optical Imaging with Radioactive Probes

Hongguang Liu; Gang Ren; Zheng Miao; Xiaofen Zhang; Xiaodong Tang; Peizhen Han; Gambhir, Sanjiv S.; Zhen Cheng
March 2010
PLoS ONE;2010, Vol. 5 Issue 3, p1
Academic Journal
Background: Optical imaging (OI) techniques such as bioluminescence and fluorescence imaging have been widely used to track diseases in a non-invasive manner within living subjects. These techniques generally require bioluminescent and fluorescent probes. Here we demonstrate the feasibility of using radioactive probes for in vivo molecular OI. Methodology/Principal Findings: By taking the advantages of low energy window of light (1.2-3.1 eV, 400-1000 nm) resulting from radiation, radionuclides that emit charged particles such as β+ and β- can be successfully imaged with an OI instrument. In vivo optical images can be obtained for several radioactive probes including 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG), Na18F, Na131I, 90YCl3 and a 90Y labeled peptide that specifically target tumors. Conclusions/Significance: These studies demonstrate generalizability of radioactive OI technique. It provides a new molecular imaging strategy and will likely have significant impact on both small animal and clinical imaging.


Related Articles

  • Fluorescent Probes for Nucleic Acid Visualization in Fixed and Live Cells. Boutorine, Alexandre S.; Novopashina, Darya S.; Krasheninina, Olga A.; Nozeret, Karine; Venyaminova, Alya G. // Molecules;Dec2013, Vol. 18 Issue 12, p15357 

    This review analyses the literature concerning non-fluorescent and fluorescent probes for nucleic acid imaging in fixed and living cells from the point of view of their suitability for imaging intracellular native RNA and DNA. Attention is mainly paid to fluorescent probes for fluorescence...

  • Synthesis and fluorescent properties of a novel dansyl-based fluorescent probe for Hg2+. Zuo-An, Xiao; Dan, Zhan; Xiao, Yang // Journal of Chemical Research;Feb2014, Vol. 38 Issue 2, p108 

    A novel turn-off fluorescent probe containing a dansyl fluorophore has been synthesised. Its recognition properties towards various metal ions have been studied by fluorescence spectrometry. The compound showed a high sensitivity and selectivity to Hg2+ ion and a complexation ratio towards Hg2+...

  • Sensitive bioanalysis—combining single-molecule spectroscopy with mono-labeled self-quenching probes. Marmé, Nicole; Knemeyer, Jens-Peter // Analytical & Bioanalytical Chemistry;Jul2007, Vol. 388 Issue 5/6, p1075 

    Fluorescence single-molecule spectroscopy is an appropriate tool for modern bioanalysis. This technique enables the development of ultra sensitive assays, especially when combined with self-quenching probes. In this review we report novel DNA, enzyme, and antibody assays based on mono-labeled...

  • Fluorescent properties and symmetry of the monodeprotonated form of 5,10,15,20-tetrakis-(4- N-methylpyridyl)-porphyrin. Kruk, N. N. // Journal of Applied Spectroscopy;Sep2006, Vol. 73 Issue 5, p686 

    The spectral and kinetic properties of the monodeprotonated form of the cationic 5,10,15,20-tetrakis(4-Nmethylpyridyl) porphyrin (H2TMPyP) in solution are studied. The experimental results obtained are interpreted based on the four-orbital Gouterman model. It is established that the...

  • A Pyrene- and Phosphonate-Containing Fluorescent Probe as Guest Molecule in a Host Polymer Matrix. Villemin, Elise; Elias, Benjamin; Devillers, Michel; Marchand-Brynaert, Jacqueline // Molecules;Feb2013, Vol. 18 Issue 2, p1897 

    New host-guest materials have been prepared by incorporation of a home-made organic probe displaying a pyrene motif and a phosphonate function into a regular amphiphilic copolymer. Using powder X-Ray diffraction, photoluminescence and FT-IR spectroscopy, we have been able to study the...

  • Sucrose Monoester Micelles Size Determined by Fluorescence Correlation Spectroscopy (FCS). Sanchez, Susana A.; Gratton, Enrico; Zanocco, Antonio L.; Lemp, Else; Gunther, German // PLoS ONE;2011, Vol. 6 Issue 12, p1 

    One of the several uses of sucrose detergents, as well as other micelle forming detergents, is the solubilization of different membrane proteins. Accurate knowledge of the micelle properties, including size and shape, are needed to optimize the surfactant conditions for protein purification and...

  • Synthesis of a Novel l-Methyl-Methionine-ICG-Der-02 Fluorescent Probe for In Vivo Near Infrared Imaging of Tumors. Mahounga, Didel; Shan, Lingling; Jie, Cao; Du, Changli; Wan, Shunan; Gu, Yueqing // Molecular Imaging & Biology;Dec2012, Vol. 14 Issue 6, p699 

    Purpose: A novel near infrared fluorescent probe, l-methyl-methionine (Met)-ICG-Der-02, was synthesized and characterized for in vivo imaging of tumors and early diagnosis of cancers. Method: Met was conjugated with ICG-Der-02 dye through the amide bond function by...

  • Numerical Modeling of Bioluminescence Distributions in the Coastal Ocean. Shulman, Igor; Haddock, Steven H. D.; McGillicuddy, Dennis J.; Paduan, Jeffrey D.; Bissett, W. Paul // Journal of Atmospheric & Oceanic Technology;Jul2003, Vol. 20 Issue 7, p1060 

    Bioluminescence (BL) predictability experiments (predictions of the intensity, depth, and distance offshore of the BL maximum) were conducted using an advective-diffusive tracer model with velocities and diffusivities from a fine-resolution model of the Monterey Bay, California, area. For tracer...

  • Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. Gurskaya, Nadya G.; Verkhusha, Vladislav V.; Shcheglov, Alexander S.; Staroverov, Dmitry B.; Chepurnykh, Tatyana V.; Fradkov, Arkady F.; Lukyanov, Sergey; Lukyanov, Konstantin A. // Nature Biotechnology;Apr2006, Vol. 24 Issue 4, p461 

    Green fluorescent protein (GFP) and GFP-like proteins represent invaluable genetically encoded fluorescent probes. In the last few years a new class of photoactivatable fluorescent proteins (PAFPs) capable of pronounced light-induced spectral changes have been developed. Except for tetrameric...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics