TITLE

Specific Cell Targeting for Delivery of Toxins into Small-Cell Lung Cancer Using a Streptavidin Fusion Protein Complex

AUTHOR(S)
Yu, Adam; Choi, Jung; Ohno, Kouichi; Levin, Brandi; Rom, William N.; Meruelo, Daniel
PUB. DATE
July 2000
SOURCE
DNA & Cell Biology;Jul2000, Vol. 19 Issue 7, p383
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
New modalities of treatment for small-cell lung cancer (SCLC) are needed, because the majority of patients continue to die of disseminated disease despite an initial response to conventional chemotherapy. Abnormal surface expression of the neural-cell adhesion molecule (NCAM) has been noted to be highly associated with SCLC. We examined the ability and efficiency of a streptavidin�Protein A (ST-PA) fusion protein complexed with an anti-NCAM monoclonal antibody (Mab) to transfer biotinylated � -galactosidase into human SCLC cell lines NCI-H69, NCI-H526, and NCI-H446. When the surface molecule NCAM was targeted with this system, more than 99% of the targeted cells internalized and exhibited � -galactosidase activity. In addition, we evaluated cytotoxic activity against SCLC lines NCI-H69 and NCI-H526 by efficient delivery of biotinylated glucose oxidase using the same ST-PA/anti-NCAM Mab complex. Cytotoxicity of the transduced cells (SCLC) was 10-fold and 100-fold greater, respectively, than the glucose oxidase control. This system could be widely applied for specific therapy of cancer cells by targeting unique surface molecules (antigens) using the corresponding Mab/ST-PA complex to transfer a variety of effector molecules; e.g., immunotoxic compounds, into target cells with a high degree of efficiency and specificity.
ACCESSION #
5583821

 

Related Articles

  • No improved response in advanced NSCLC when TRAIL-R1 agonist mAb added to chemotherapy combo.  // Hem/Onc Today;7/10/2010, Vol. 10 Issue 13, p20 

    The article discusses a phase 2 trial which found that including the TRAIL-R1 agonist monoclonal antibody mapatumumab to paclitaxel/carboplatin did not enhance response or progression free survival (PFS) in patients suffering from advanced non-small cell lung cancer.

  • Cetuximab: a new hope for advanced NSCLC. Karmali, S. // Thorax;Oct2009, Vol. 64 Issue 10, p907 

    The article focuses on a randomized study which investigates a monoclonal antibody against the epidermal growth factor receptor (EGFR), cetuximab, as a treatment option for advanced non-small cell lung cancers. The study randomly assigns patients to chemotherapy with cetuximab or chemotherapy...

  • Combinational Therapy Enhances the Effects of Anti-IGF-1R mAb Figitumumab to Target Small Cell Lung Cancer. Cao, Hongxin; Dong, Wei; Shen, Hongchang; Xu, Jun; Zhu, Linhai; Liu, Qi; Du, Jiajun // PLoS ONE;8/19/2015, Vol. 10 Issue 8, p1 

    Background: Small cell lung cancer (SCLC) is a recalcitrant malignancy with distinct biologic properties. Antibody targeting therapy has been actively investigated as a new drug modality. Methods: We tested the expression of IGF-1R and calculated the survival in 61 SCLC patients. We also...

  • Bevacizumab in Non Small Cell Lung Cancer: Development, Current Status and Issues. Sandomenico, C.; Costanzo, R.; Carillio, G.; Piccirillo, M. C.; Montanino, A.; Di Maio, M.; Rocco, G.; Normanno, N.; Perrone, F.; Morabito, A. // Current Medicinal Chemistry;Mar2012, Vol. 19 Issue 7, p961 

    Bevacizumab is a monoclonal antibody directed against Vascular Endothelial Growth Factor (VEGF). Evidence about its efficacy in addition to first-line chemotherapy in non-small-cell-lung-cancer (NSCLC) has been produced by two large randomized phase III clinical trials (ECOG 4599 and AVAiL),...

  • Practical management of NSCLC patients with long-term bevacizumab treatment: a report of four cases. Herder, G. J. M.; Codrington, H.; Colder, C. D.; Aerts, J. G. // Lung Cancer: Targets & Therapy;2013, Vol. 4, p55 

    Introduction: Previous research showed that the addition of bevacizumab (a monoclonal antibody against vascular endothelial growth factor [VEGF]) to chemotherapy resulted in a significant efficacy benefit in the treatment of selected patients with advanced nonsquamous non-small cell lung cancer...

  • Bevacizumab-Induced Hypertension. Syrigos, Kostas N.; Karapanagiotou, Eleni; Boura, Paraskevi; Manegold, Christian; Harrin, Kevin // BioDrugs;2011, Vol. 25 Issue 3, p159 

    Bevacizumab, a recombinant humanized monoclonal antibody targeting the vascular endothelial growth factor (VEGF), has been approved in the US as first- and second-line treatment of colorectal cancer and in the first-line treatment of advanced non-small cell lung cancer. The US FDA has also...

  • Targeting Angiogenesis with Multitargeted Tyrosine Kinase Inhibitors in the Treatment of Non-Small Cell Lung Cancer. Scagliotti, Giorgio; Govindan, Ramaswamy // Oncologist;May2010, Vol. 15 Issue 5, p436 

    It has been >35 years since the link between angiogenesis and the growth of tumors was first reported. Targeting angiogenesis became feasible with the availability of bevacizumab, an anti-vascular endothelial growth factor monoclonal antibody. Initial studies revealed that the combination of...

  • Advances of Treatment of Small Cell Lung Cancer in 2012 American Society of Clinical Oncology Annual Meeting. Kan Wu; Bing Xia; Shenglin Ma // Chinese Journal of Lung Cancer;Nov2012, Vol. 15 Issue 11, p672 

    No abstract available.

  • Lung cancer imaging agent.  // FDA Consumer;Nov96, Vol. 30 Issue 9, p5 

    Announces the availability of the Nofetumomab, an agent that can determine the extent of disease in patients diagnosed with small cell lung cancer (SCLC). Benefits in using the product; Restrictions on the use of the product; Statistics on the number of patients diagnosed with SCLC in the...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics