Thermostability Profile of Newcastle Disease Viruses Isolated from Wild Birds in Central Nigeria and the Selection of a Thermostable Clone from the Sub-Population

Ibu, O. J.; Okoye, J. O. A.; Baba, S. S.; Soyinka, S. V. O.; Chah, K. F.; Antiabong, J.; Eze, D.; Salihu, E. A.; Oladele, S. B.
August 2010
International Journal of Poultry Science;2010, Vol. 9 Issue 8, p791
Academic Journal
The study was carried out to assess the Haemagglutinin thermostability of Newcastle disease virus isolates obtained from wild birds in three climatically distinct states in central Nigeria. Identification of heat stable ND virus isolates from the locality will provide environmentally friendly thermostable vaccine candidates for rural poultry. The 12 field virus isolates and the 5 vaccine virus strains showed variable degrees of heat stability. Three field isolates each was inactivated in 5 mm, three in 10 mm and one in 15 mm. One isolate was inactivated in 20 mm while two and three strains got inactivated in 25 and 30 mm respectively. The most thermostable of the field isolates was inactivated in 40 mm. A more thermostable clone was subsequently derived from the latter strain as a local vaccine candidate. For the vaccine strains, NDV (I/O) and NDV (K) were inactivated in 20 mm while NDV (L) was inactivated in 25 mm. The velogenic strain (Herts) was inactivated in 40 mm. The two established thermostable strains, NDV4 and NDVI2 were inactivated in 90 mm each. The thermostable profile of the field virus strains did not vary with the climatic background of the isolates.


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