Effects of inosine on reperfusion injury after cardiopulmonary bypass

Veres, Gábor; Radovits, Tamás; Seres, Leila; Horkay, Ferenc; Karck, Matthias; Szabó, Gábor
January 2010
Journal of Cardiothoracic Surgery;2010, Vol. 5, p106
Academic Journal
Objective: Inosine, a break-down product of adenosine has been recently shown to exert inodilatory and antiinflammatory properties. Furthermore inosine might be a key substrate of pharmacological post-conditioning. In the present pre-clinical study, we investigated the effects of inosine on cardiac function during reperfusion in an experimental model of cardioplegic arrest and extracorporal circulation. Methods: Twelve anesthetized dogs underwent hypothermic cardiopulmonary bypass. After 60 minutes of hypothermic cardiac arrest, reperfusion was started after application of either saline vehicle (control, n = 6), or inosine (100 mg/kg, n = 6). Left ventricular end-systolic pressure volume relationship (ESPVR) was measured by a combined pressure-volume-conductance catheter at baseline and after 60 minutes of reperfusion. Left anterior descendent coronary blood flow (CBF), endothelium-dependent vasodilatation to acetylcholine (ACh) and endothelium-independent vasodilatation to sodium nitroprusside (SNP) were also determined. Results: The administration of inosine led to a significantly better recovery (given as percent of baseline) of ESPVR 90 ± 9% vs. 46 ± 6%, p < 0.05. CBF and was also significantly higher in the inosine group (56 ± 8 vs. 23 ± 4, ml/ min, p < 0.05). While the vasodilatatory response to SNP was similar in both groups, ACh resulted in a significantly higher increase in CBF (58 ± 6% vs. 25 ± 5%, p < 0.05) in the inosine group. Conclusions: Application of inosine improves myocardial and endothelial function after cardiopulmonary bypass with hypothermic cardiac arrest.


Related Articles

  • Adipocytokine resistin correlates with oxidative stress and myocardial injury in patients undergoing cardiac surgery. Laurikka, Antti; Vuolteenaho, Katriina; Toikkanen, Vesa; Rinne, Timo; Leppänen, Tiina; Tarkka, Matti; Laurikka, Jari; Moilanen, Eeva // European Journal of Cardio-Thoracic Surgery;Oct2014, Vol. 46 Issue 4, p729 

    OBJECTIVES Adipocytokines are hormones regulating energy metabolism and appetite and according to recent reports also inflammatory responses including ischaemia–reperfusion injury. Based on experimental data, we hypothesized that the levels of adipocytokines adiponectin, adipsin, leptin...

  • Whole Blood Transcriptomics in Cardiac Surgery Identifies a Gene Regulatory Network Connecting Ischemia Reperfusion with Systemic Inflammation. Liangos, Orfeas; Domhan, Sophie; Schwager, Christian; Zeier, Martin; Huber, Peter E.; Addabbo, Francesco; Goligorsky, Michael S.; Hlatky, Lynn; Jaber, Bertrand L.; Abdollahi, Amir // PLoS ONE;2010, Vol. 5 Issue 10, p1 

    Background: Cardiac surgery with cardiopulmonary bypass (CS/CPB) is associated with increased risk for postoperative complications causing substantial morbidity and mortality. To identify the molecular mechanisms underlying CS/CPB-induced pathophysiology we employed an integrative systems...

  • Pre-conditioning with the soluble guanylate cyclase activator Cinaciguat reduces ischaemia–reperfusion injury after cardiopulmonary bypass Radovits, Tamás; Korkmaz, Sevil; Miesel-Gröschel, Christiane; Seidel, Beatrice; Stasch, Johannes-Peter; Merkely, Béla; Karck, Matthias; Szabó, Gábor // European Journal of Cardio-Thoracic Surgery;Feb2011, Vol. 39 Issue 2, p248 

    Abstract: Objective: Activation of the nitric oxide–soluble guanylate cyclase–cyclic guanosine monophosphate (NO–sGC–cGMP) pathway can induce potent cardioprotection-like effects against ischaemia–reperfusion injury and nitro-oxidative stress. We investigated...

  • Changes in metabolism and blood flow in peripheral tissue (skeletal muscle) during cardiac surgery with cardiopulmonary bypass: the biochemical microdialysis study. Mand'ák, J.; Živný, P.; Lonský, V.; Palička, V.; Kakrdová, D.; Marśíková, M.; Kuneš, P.; Kubíček, J. // Perfusion;Jan2004, Vol. 19 Issue 1, p53 

    The aim of this study was to monitor the metabolism and blood flow in the interstitium of the skeletal muscle during cardiac surgery with cardiopulmonary bypass (CPB) and in the early postoperative period by means of microdialysis and to compare metabolic changes during CPB at normothermia (NT)...

  • Cerebral injury during paediatric heart surgery: perfusion issues. Scallan, Michael J.H. // Perfusion;Jul2004, Vol. 19 Issue 4, p221 

    Suboptimal neurodevelopmental outcome is common in children who have congenital heart disease. Its aetiology is often multifactorial. This review focuses on the role of cardiopulmonary bypass. Hypothermia is the mainstay of cerebral protection. Low flow and regional low flow are preferred to...

  • Single, percutaneous, femoral venous cannulation for cardiopulmonary bypass. Riley, William; FitzGerald, Daniel; Cohn, Lawrence // Perfusion;May2007, Vol. 22 Issue 3, p211 

    Percutaneous femoral venous cannulation for cardiopulmonary bypass has emerged as an indispensable technique in the management of cardiac surgical procedures requiring cardiopulmonary bypass. A review of cases at Brigham and Women's Hospital (Boston, MA, USA) relying solely on percutaneous...

  • A preliminary study about the effects of warm priming solution on oxidative stress and postoperative atrial fibrillation in open heart surgery. Buyukates, Mustafa; Acikgoz, Serefden; Aktunc, Erol; Kandemir, Ozer; Dogan, Sait Mesut; Aydin, Mustafa // Wiener Klinische Wochenschrift;Sep2012, Vol. 124 Issue 17/18, p618 

    Background: An important reason for production of ischemia and reperfusion injury and oxidative stress is the sudden and rapid changes in body temperature during the institution of cardiopulmonary bypass. The aim of this study was to investigate the effects of warm priming solution on oxidative...

  • Ischemic Intestinal Injury during Cardiopulmonary Bypass Does Not Show an Association with Neutrophil Activation. Jormalainen, M.; Vento, A. E.; Wartiovaara-Kautto, U.; Suojaranta-Ylinen, R.; Lauronen, J.; Paavonen, T.; Petäjä, J. // European Surgical Research;2009, Vol. 42 Issue 1, p59 

    Background: Neutrophil activation and tissue sequestration are crucial events in intestinal ischemia-reperfusion injury, but their role in the gut wall after clinical cardiopulmonary bypass (CPB) remains unclear. We tested whether local post-CPB inflammatory response in the gut wall would be...

  • Effect of dexmedetomidine on myocardial ischemia-reperfusion injury. Shoulin Chen; Fuzhou Hua; Jun Lu; Yu Jiang; Yanhua Tang; Lei Tao; Bing Zou; Qinghua Wu // International Journal of Clinical & Experimental Medicine;2015, Vol. 8 Issue 11, p21166 

    Cardiopulmonary bypass (CPB) is associated with a marked systemic inflammatory response. Although dexmedetomidine (Dex) is routinely used in cardiac surgery, the effect in reducing the inflammatory response in coronary artery bypass graft surgery (CABG) with CPB remains unclear. In this study,...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics