Immature Cryopreserved Ovary Restores Puberty and Fertility in Mice without Alteration of Epigenetic Marks

Sauvat, Frédé rique; Capito, Carmen; Sarnacki, Sabine; Poirot, Catherine; Bachelot, Anne; Meduri, Geri; Dandolo, Luisa; Binart, Nadine
April 2008
PLoS ONE;2008, Vol. 3 Issue 4, p1
Academic Journal
Background: Progress in oncology could improve survival rate in children, but would probably lead to impaired fertility and puberty. In pre-pubertal girls, the only therapeutic option is the cryopreservation of one ovary. Three births have been reported after reimplantation of cryopreserved mature ovary. Conversely, reimplantation of ovary preserved before puberty (defined as immature ovary) has never been performed in humans. Methodology/Principal Findings: In order to analyze ovarian function, we performed transplantation using fresh or cryopreserved immature grafts in pre-pubertal or adult mice. Puberty as well as cyclic hormonal activity was restored. All follicle populations were present although a significant reduction in follicle density was observed with or without cryopreservation. Although fertility was restored, the graft is of limited life span. Because ex vivo ovary manipulation and cryopreservation procedure, the status of genomic imprinting was investigated. Methylation status of the H19 and Lit1 Imprinting Control Regions in kidney, muscle and tongue of offsprings from grafted mice does not show significant alteration when compared to those of unoperated mice. Conclusions/Significance: These results demonstrate that immature ovarian grafting can restore spontaneous puberty and fertility. However, these data suggest that follicle depletion leads to premature ovarian failure. This study addresses the very important epigenetics issue, and provides valuable information to the study of ovarian transplantation suggesting that these procedures do not perturb normal epigenetics marks. These results are highly relevant to the reimplantation question of immature cortex in women.


Related Articles

  • Mouse Ovarian Tissue Cryopreservation Has Only a Minor Effect on In Vitro Follicular Maturation and Gene Expression. Hung-Ching Liu; Zhiming He, Larry I.; Rosenwaks, Zev // Journal of Assisted Reproduction & Genetics;Oct2003, Vol. 20 Issue 10, p421 

    Purpose: To establish a protocol for ovarian tissue cryopreservation which can retain fertility potential after thawing and to evaluate the impact of cryopreservation on development and gene expression during folliculogenesis. Methods: A controlled randomized study in a clinical and academic...

  • Density and distribution of primordial follicles in single pieces of cortex from 21 patients and in individual pieces of cortex from three entire human ovaries. K.L.T. Schmidt; A.G. Byskov; A. Nyboe Andersen; J. Müller; C. Yding Andersen // Human Reproduction;Jun2003, Vol. 18 Issue 6, p1158 

    BACKGROUND: At the time of cryopreservation of ovarian tissue for fertility preservation a small biopsy of ovarian cortex is usually taken for histological evaluation of the follicular reserve. The purpose of this study was to evaluate the distribution and density of primordial follicles in...

  • Effects of Vitamin A on In Vitro Maturation of Pre-Pubertal Mouse Spermatogonial Stem Cells. Travers, Albanne; Arkoun, Brahim; Safsaf, Athmane; Milazzo, Jean-Pierre; Absyte, Anne; Bironneau, Amandine; Perdrix, Anne; Sibert, Louis; Macé, Bertrand; Cauliez, Bruno; Rives, Nathalie // PLoS ONE;Dec2013, Vol. 8 Issue 12, p1 

    Testicular tissue cryopreservation is the only potential option for fertility preservation in pre-pubertal boys exposed to gonadotoxic treatment. Completion of spermatogenesis after in vitro maturation is one of the future uses of harvested testicular tissue. The purpose of the current study was...

  • Efficacy of ovarian tissue cryopreservation in a major European center. Bastings, L.; Liebenthron, J.; Westphal, J.; Beerendonk, C.; Ven, H.; Meinecke, B.; Montag, M.; Braat, D.; Peek, R. // Journal of Assisted Reproduction & Genetics;Aug2014, Vol. 31 Issue 8, p1003 

    Purpose: To evaluate the effect of cryopreservation and thawing of ovarian tissue from oncological patients opting for fertility preservation on ovarian tissue viability. Methods: In this prospective cohort study, the ovarian tissue viability before and after cryopreservation and thawing was...

  • Evaluation of MMP-2 gene expression in mice pre-antral follicles derived from vitrified and fresh ovarian tissue. Khosravi, S.; Zavareh, S.; Paylakhi, S.; Gorbanian, M. // Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p84 

    Introduction: Cryopreservation is a useful method for preservation of ovarian tissue of patients who are candidate for chemo and radiotherapy. It has been postulated that MMP-2 participates in the process of remodeling of ovarian extracellular matrix such as the follicle growth and ovulation....

  • OPTIMUM FOR CRYOPRESERVATION OF HUMAN OVARIAN TISSUE: GENE EXPRESSION AS A DETECTOR OF EFFECTIVENESS. Isachenko, V.; Lapidus, I.; Isachenko, E.; Hancke, K.; Nawroth, F.; Wiegratz, I.; Kreienberg, R. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Oct2010 S3 Supplemen, Vol. 20, pS54 

    Objective: Cryopreservation, the most important stage of the cryobanking of ovarian tissue, can be carried out using one of two methods: conventional ("slow") freezing, and vitrification (direct plunging into liquid nitrogen). For human oocytes vitrification is more effective compared to...

  • Conference Abstracts ... April 28, 2008.  // Fertility Weekly;4/28/2008, p11 

    The article presents abstracts of research related to fertility, including the role of estrogen receptors (ER) in ovarian physiology, the advantages and disadvantages of vitrification as a cryopreservation method, and the effect of stimulation with recombinant FSH and GnRH antagonist on...

  • Correlation between Ovarian Graft Function and AMH and Inhibin B Levels after Gonadotoxic Treatment.  // Fertility Weekly;11/14/2011, p5 

    The article presents a study conducted by F. Janse and colleagues in the Netherlands which examines markers of ovarian reserve and ovarian function after gonadotoxic treatment. The study discovers anti-Mullerian hormone (AMH) and inhibin B levels can not be considered as markers of ovarian...

  • Fertility Preservation and Oocyte Genetics.  // Turkish Policy Quarterly;2009, Vol. 8 Issue 4, p1 

    The article discusses research on the viability of fertility preservation and normal oocytes in women with mosaic Turner syndrome. It references a study by A. H. Balen and colleagues in a 2010 issue of the "British Journal of Obstetrics and Gynaecology." The case involved a short-statured female...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics