TITLE

Intestinal Colonization of IL-2 Deficient Mice with Non- olitogenic B. vulgatus Prevents DC Maturation and T-Cell Polarization

AUTHOR(S)
Müller, Martina; Fink, Kerstin; Geisel, Julia; Kahl, Frauke; Jilge, Burghardt; Reimann, Jörg; Mach, Nicolas; Autenrieth, Ingo B.; Frick, Julia S.
PUB. DATE
June 2008
SOURCE
PLoS ONE;2008, Vol. 3 Issue 6, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: IL-2 deficient (IL-2-1-) mice mono-colonized with E. coli mpk develop colitis whereas IL-2-1--mice monocolonized with B. vulgatus mpk do not and are even protected from E. coli mpk induced colitis. Methodology/Principal Findings: We investigated if mono-colonization with E. coli mpk or B. vulgatus mpk differentially modulates distribution, activation and maturation of intestinal lamina propria (LP) dendritic cells (DC). LP DC in mice monocolonized with protective B. vulgatus mpk or co-colonized with E. coli mpk/B. vulgatus mpk featured a semi-mature LP DC phenotype (CD40loCD80loMHC-IIhi) whereas mono-colonization with colitogenic E. coli mpk induced LP DC activation and maturation prior to onset of colitis. Accordingly, chemokine receptor (CCR) 7 surface expression was more strikingly enhanced in mesenteric lymph node DC from E. coli mpk than B. vulgatus mpk mono- or co-colonized mice. Mature but not semi-mature LP DC promoted Th1 polarization. As B. vulgatus mpk promotes differentiation of semi-mature DC presumably by IL-6, mRNA and protein expression of IL-6 was investigated in LP DC. The data demonstrated that IL-6 mRNA and protein was increased in LP DC of B. vulgatus mpk as compared to E. coli mpk mono-colonized IL-2-1--mice. The B. vulgatus mpk mediated suppression of CCR7 expression and DC migration was abolished in IL-6-1--DC in vitro. Conclusions/Significance: From this data we conclude that the B. vulgatus triggered IL-6 secretion by LP DC in absence of proinflammatory cytokines such as IL-12 or TNF-α induces a semi-mature LP DC phenotype, which might prevent T-cell activation and thereby the induction of colitis in IL-2-1--mice. The data provide new evidence that IL-6 might act as an immune regulatory cytokine in the mucosa by targeting intestinal DC.
ACCESSION #
55425856

 

Related Articles

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics