TITLE

Comparison of isoelectric focusing and polymerase chain reaction for the detection of β-lactamases

AUTHOR(S)
Sharma, J.; Ray, P.; Sharma, M.; Ghosh, S.
PUB. DATE
October 2010
SOURCE
Indian Journal of Medical Microbiology;Oct2010, Vol. 28 Issue 4, p376
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Extended spectrum β-lactamases (ESBLs) have been observed in virtually all the species of family Enterobacteriaceae. Threat posed by antibiotic resistance because of ESBLs is more serious as a number of technical problems are associated with the detection of these enzymes. Although a number of detection methods have been designed for ESBLs, every method has its own benefits and shortcomings as well. In earlier days, isoelectric focusing (IEF) was used as the gold standard for ESBL detection. This study was undertaken to compare IEF with polymerase chain reaction, a method which has been extensively used for ESBL detection these days.
ACCESSION #
55249701

 

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