TITLE

IgG - Indirect fluorescent antibody technique to detect seroprevalence of Toxoplasma gondii in immunocompetent and immunodeficient patients in southern districts of Tamil Nadia

AUTHOR(S)
Sucilathangam, G.; Palaniappan, N.; Sreekumar, C.; Anna, T.
PUB. DATE
October 2010
SOURCE
Indian Journal of Medical Microbiology;Oct2010, Vol. 28 Issue 4, p354
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose: The purpose of the study was to determine the prevalence of Toxoplasma antibodies in selected immunocompetent and immunodeficient patients in and around Tirunelveli District of Tamil Nadu. Materials and Methods: This study was carried out from May 2006 to October 2007 in 175 immunodeficient and 175 immunocompetent patients. Serum samples were subjected into in-house IgG assay using indirect fluorescent antibody test (IFAT). Results: Out of 350 patients tested by IgG IFAT, 41 (11.71%) had antibodies for Toxoplasma gondii with a mean IFA titre of 43.42 ± 58.7 and the titre ranging from 1 : 16 to 1 : 256. Among the immunocompetent and immunodeficient groups, 19 patients (10.86%) and 22 patients (12.57%), respectively, had antibodies to T. gondii. Various risk factors associated within the study group was analysed and results were interpreted. Conclusions: The study has highlighted an overall seroprevalence of 11.71% with 12.57% in immunocompromised and 10.86% in immunocompetent patients respectively in a southern district, Tamil Nadu, which underlines the importance of screening of this parasite especially in the immunocompromised patients.
ACCESSION #
55249695

 

Related Articles

  • EVALUATION OF TWO TOXOPLASMA GONDI! SEROLOGIC TESTS USED IN A SEROSURVEY OF DOMESTIC CATS IN CALIFORNIA. Dabritz, Haydee A.; Gardner, Ian A.; Miller, Melissa A.; Lappin, Michael R.; Atwill, E. Robert; Packham, Andrea E.; Melli, Ann C.; Conrad, Patricia A. // Journal of Parasitology;Aug2007, Vol. 93 Issue 4, p806 

    The article evaluates the sensitivity and specificity of the Immunoglobulin G (IgG) enzyme-linked immunoabsorbent assay (ELISA) and IgG indirect fluorescent antibody test (IFAT) in detecting Toxoplasma gondii-specific antibodies in two domestic cats in California, using the Bayesian approach....

  • Comparison of indirect fluorescent antibody test and modified agglutination test for detecting Toxoplasma gondii immunoglobulin G antibodies in dog and cat. Macrì, Gladia; Sala, Marcello; Linder, Alicia; Pettirossi, Nadia; Scarpulla, Manuela // Parasitology Research;Jul2009, Vol. 105 Issue 1, p35 

    Abstract  The present study describes the comparison between a modified agglutination test (MAT) and the indirect fluorescent antibody test (IFAT) for the detection of Toxoplasma specific IgG antibodies in dog and cat sera. MAT showed an “almost perfect” agreement with IFAT in...

  • Seroprevalence of Toxoplasmosis in the Residents of Cheorwon-gun, Gangwon-do, Korea. Hye-Jin Ahn; Pyo Yun Cho; Seong Kyu Ahn; Tong-Soo Kim; Chom-Kyu Chong; Sung-Jong Hong; Seok Ho Cha; Ho-Woo Nam // Korean Journal of Parasitology;Sep2012, Vol. 50 Issue 3, p225 

    The seroepidemiological status of toxoplasmosis was surveyed among the residents of Cheorwon-gun, Gangwon- do by means of ELISA using a crude extract antigen of Toxoplasma gondii. The sera of 1,661 adult residents (866 males and 795 females) were collected and checked for IgG antibody titers,...

  • Seroepidemiological study of toxoplasmosis in sheep in rural areas of the Grosseto district, Tuscany, Italy. Sechi, Paola; Ciampelli, Antonio; Cambiotti, Valentina; Veronesi, Fabrizia; Cenci-Goga, Beniamino T. // Italian Journal of Animal Science;2013, Vol. 12 Issue 2, p236 

    Six hundred and thirty individual serum samples from dairy sheep were tested for the presence of antibodies to Toxoplasma gondii using an indirect immunofluorescence antibody test (IFAT). The sampled animals came from 33 dairy herds representative of the southern area of the Tuscany region....

  • Maintained Seroprevalence of Toxoplasmosis among the Residents of Jeju Island, Korea. Sung-Jong Hong; Chom-Kyu Chong; Kyutaeg Lee; Tong-Soo Kim; Yeon-Pyo Hong; Hye-Jin Ahn; Hak-Yong Kim; A-Ra Ko; Yong-Joo Kim; Ho-Woo Nam // Korean Journal of Parasitology;Sep2011, Vol. 49 Issue 3, p309 

    Seroepidemiological status of toxoplasmosis among the residents of Jeju island was surveyed and evaluated by ELISA with crude extract of Toxoplasma gondii. The sera of 2,348 residents (male 1,157 and female 1,191) were collected and checked for the IgG antibody titers, which showed 13.2%...

  • First description of naturally acquired Tritrichomonas foetus infection in a Persian cattery in Spain. Miró, Guadalupe; Hernández, Leticia; Montoya, Ana; Arranz-Solís, David; Dado, Diana; Rojo-Montejo, Silvia; Mendoza-Ibarra, Jesús; Ortega-Mora, Luís; Pedraza-Díaz, Susana // Parasitology Research;Oct2011, Vol. 109 Issue 4, p1151 

    Tritrichomonas foetus has been identified as the causative agent of feline intestinal trichomonosis, characterized by clinical signs of chronic large bowel diarrhoea. This disease has been reported in cats from the USA, Europe and Australia. However, its epidemiology is still unclear. The aim of...

  • Neutralization assay to resolve discrepancies between positive results in new highly sensitive anti- Toxoplasma gondii IgG assays and negative results in reference tests. Köhler, S.; Rößler, D.; Hornauer, S.; Upmeier, B.; Franck, J.; Liesenfeld, O. // European Journal of Clinical Microbiology & Infectious Diseases;Mar2010, Vol. 29 Issue 3, p359 

    High sensitivity of IgG assays is required in order to diagnose Toxoplasma infection in immunocompromised patients and women before and during early pregnancy. Positive results obtained in new highly sensitive Toxoplasma IgG assays have been observed in sera that gave negative results in...

  • Anti-Toxoplasma gondii Antibodies in Haemodialysis Patients. Solhjoo, Kavous; Jahromi, Abdolreza Sotoodeh; Parnian-Rad, Ayeh // American Journal of Infectious Diseases;2010, Vol. 6 Issue 1, p13 

    Problem statement: In situations of immunodeficiency, Toxoplasma gondii emerges as a life-threatening infection. Toxoplasma gondii is transmitted parenterally, flourish in immunocompromised subjects and, most toxoplasma infections are asymptomatic. Studies have shown that there is a...

  • Low Sero-Prevalence of Toxoplasma gondii IgG and IgM Antibodies in HIV Sero-Positive Patients Attending National Hospital, Abuja, Nigeria. Mirabeau, Tatfeng Youtchou; Ebikade, Adesua Ojo // Journal of Life Sciences;May2012, Vol. 6 Issue 5, p481 

    Several researchers have investigated the association of numerous opportunistic pathogens with HIV, little is documented on its association with T. gondii in our environment. We investigated the prevalence of T. gondii immunoglobulins G and M (IgG and IgM) in HIV positive individuals in relation...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics