Hydrolytic Enzyme Production by Aspergillus niger in Solid State Fermentation: Stimulation of Transcriptional Regulation Through Carbon and Nitrogen Supplementation

McKelvey, Shauna M.; Murphy, Richard A.
June 2010
BioProcessing Journal;Jun2010, Vol. 9 Issue 1, p38
Academic Journal
The article examines the influence of carbon and nitrogen sources on the production and transcriptional regulation of cellulase, xylanase, and protease created by Aspergillus niger under solid-state condition. The application of real-time polymerase chain reaction (PCR) techniques to clarify the control mechanisms and pathways involved in nutrient metabolism are discussed. One fungal strain was separated for further research and the application of enzymatic and molecular analysis are presented. Molecular techniques such as the RNA extraction and reverse transcription are discussed.


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