TITLE

Nitric oxide inhibits ghrelin-induced cell proliferation and ERK1/2 activation in GH3 cells

AUTHOR(S)
Tian, Chunlei; Ye, Fei; Wang, Lei; Deng, Yuanguo; Dong, Yuanxun; Wang, Xiaodan; Xu, Tongjiang; Lei, Ting; Wang, Xiongwei
PUB. DATE
December 2010
SOURCE
Endocrine (1355008X);Dec2010, Vol. 38 Issue 3, p412
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Ghrelin stimulates growth hormone release and cell proliferation, which strongly supports a significant role for this peptide in the control of growth hormone-releasing adenomas function and growth. Nitric oxide can influence the stimulatory effects of ghrelin on growth hormone secretion in growth hormone-releasing adenomas. However, the effect of nitric oxide (NO) on ghrelin-induced cell proliferation and the mechanism of this effect in the adenoma were not clarified. In this study, we observed that ghrelin, at a concentration of 10 to 10 M, significantly increased BrdU incorporation into rat GH3 cells. A NO donor, S-nitroso- N-acetylpenicillamine (SNAP), blunted basal, and ghrelin-induced cell proliferation. A blocker of NO synthase, Nw-nitro- l-arginine methyl ester hydrochloride (NAME), had no influence on these actions. The activation of extracellular signal-regulated kinase (ERK) 1/2 was examined by western blotting. The results showed that SNAP reduced ghrelin-stimulated ERK1/2 activation but NAME had no influence on this activation. Together, this study indicates that NO inhibited ghrelin-induced cell proliferation by blocking ERK1/2 activation in GH3 cells.
ACCESSION #
55106594

 

Related Articles

  • Growth hormone | Clinical.  // Current Medical Literature: GH & Growth Factors;2004, Vol. 19 Issue 1, p9 

    Presents clinicians' notes about clinical growth hormone (GH). Ghrelin concentrations in health children and adolescents; Effect of acute reduction of free fatty acids by acipimox on GH-releasing hormone-induced GH secretion in type 1 diabetics; Reproducibility of GH response to sprint exercise.

  • Inhibitory Effects of GHRH Antagonists on Human GH-Secreting Adenoma Tissue. Szalontay, Luca; Benveniste, Ronald J.; Schally, Andrew V.; Vidaurre, Irving; Nadji, Mehrdad; Zarandi, Marta; Block, Norman L.; Kovacs, Magdolna // Neuroendocrinology;Jul2012, Vol. 96 Issue 1, p81 

    Experimental data indicate that antagonists of growth hormone-releasing hormone (GHRH) could be used clinically in disorders characterized by excessive GHRH/growth hormone (GH) secretion, but direct evidence for the effectiveness of GHRH antagonists on human pituitary tissue is still lacking. In...

  • The effects of coadministration of ghrelin agonist (GHRP-2) and GH on TNF-α, IL-6, and iNOS genes expression induced by LPS in mouse liver. Abrehdari, Zahra; Zendehdel, Morteza; Safarpour, Elham; Allahdini, Parvaneh // Comparative Clinical Pathology;Jul2014, Vol. 23 Issue 4, p835 

    Growth hormone (GH)-releasing peptide-2 (GHRP-2), a ghrelin receptor agonist, has been reported to bear an anti-inflammatory effect. The aim of this study is to assess the impact of GHRP-2 and GH on the expression of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and inducible...

  • Molecular Cloning and Expression Analysis of Porcine Ghrelin O-Acyltransferase. Lin, Tonghui; Meng, Qingyong; Sui, Dandan; Peng, Dezhi; Li, Yang; Liu, Xiaofang; Xie, Longfei; Li, Ning // Biochemical Genetics;Oct2011, Vol. 49 Issue 9/10, p576 

    The peptide hormone ghrelin is secreted in the stomach, with unique N-octanoylation at serine 3, which is a requirement for its functionality. These functions include growth hormone release, appetite stimulation, gastrointestinal motility, glucose regulation, and cell proliferation. The enzyme...

  • Dose-Dependent Dual Role of PIT-1 (POU1F1) in Somatolactotroph Cell Proliferation and Apoptosis. Jullien, Nicolas; Roche, Catherine; Brue, Thierry; Figarella-Branger, Dominique; Graillon, Thomas; Barlier, Anne; Herman, Jean-Paul // PLoS ONE;Mar2015, Vol. 10 Issue 3, p1 

    To test the role of wtPIT-1 (PITWT) or PIT-1 (R271W) (PIT271) in somatolactotroph cells, we established, using inducible lentiviral vectors, sublines of GH4C1 somatotroph cells that allow the blockade of the expression of endogenous PIT-1 and/or the expression of PITWT or PIT271, a dominant...

  • Lentiviral and Moloney Retroviral Expression of Green Fluorescent Protein in Somatotrophs In Vivo. Okada, Masayoshi; Matsuda, Hiroko; Okimura, Yasuhiko // PLoS ONE;Jan2013, Vol. 8 Issue 1, Special section p1 

    Previous studies have shown that the locus control region (LCR) and the promoter of the growth hormone (GH) gene can control the expression of GH. Therefore, lenti- and retro-viral vectors with these elements might be useful to monitor the activation of the GH gene and the development of newborn...

  • c-Src Regulates Akt Signaling in Response to Ghrelin via β-Arrestin Signaling-Independent and -Dependent Mechanisms. Lodeiro, Maria; Theodoropoulou, Marily; Pardo, Maria; Casanueva, Felipe F.; Camiña, Jesus P. // PLoS ONE;2009, Vol. 4 Issue 3, p1 

    The aim of the present study was to identify the signaling mechanisms to ghrelin-stimulated activation of the serine/ threonine kinase Akt. In human embryonic kidney 293 (HEK293) cells transfected with GHS-R1a, ghrelin leads to the activation of Akt through the interplay of distinct signaling...

  • Leptin Inhibits the Proliferation of Vascular Smooth Muscle Cells Induced by Angiotensin II through Nitric Oxide-Dependent Mechanisms. Rodríguez, Amaia; Gómez-Ambrosi, Javier; Catalán, Victoria; Fortuño, Ana; Frühbeck, Gema // Mediators of Inflammation;2010, Vol. 2010, p1 

    Objective. This study was designed to investigate whether leptin modifies angiotensin (Ang) II-induced proliferation of aortic vascular smooth muscle cells (VSMCs) from 10-week-old male Wistar and spontaneously hypertensive rats (SHR), and the possible role of nitric oxide (NO). Methods. NO and...

  • Ghrelin stimulates proliferation of human osteoblastic TE85 cells via NO/cGMP signaling pathway. Deng-Hu Wang; Yun-Sheng Hu; Jun-Jie Du; Yun-Yu Hu; Wei-De Zhong; Wei-Jun Qin // Endocrine (1355008X);Feb2009, Vol. 35 Issue 1, p112 

    Abstract  Ghrelin regulates bone formation and osteoblast proliferation, but the detailed signaling pathway for its action on osteoblasts remains unclear. In human osteoblastic TE85 cells, we observed the effects and intracellular signaling pathway of ghrelin on cell proliferation using...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics