TITLE

Characterization, sub-cellular localization and expression profiling of the isoprenylcysteine methylesterase gene family in Arabidopsis thaliana

AUTHOR(S)
Ping Lan; Wenfeng Li; Huizhong Wang; Wujun Ma
PUB. DATE
January 2010
SOURCE
BMC Plant Biology;2010, Vol. 10, p212
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Isoprenylcysteine methylesterases (ICME) demethylate prenylated protein in eukaryotic cell. Until now, knowledge about their molecular information, localization and expression pattern is largely unavailable in plant species. One ICME in Arabidopsis, encoded by At5g15860, has been identified recently. Over-expression of At5g15860 caused an ABA hypersensitive phenotype in transgenic Arabidopsis plants, indicating that it functions as a positive regulator of ABA signaling. Moreover, ABA induced the expression of this gene in Arabidopsis seedlings. The current study extends these findings by examining the sub-cellular localization, expression profiling, and physiological functions of ICME and two other ICME-like proteins, ICME-LIKE1 and ICME-LIKE2, which were encoded by two related genes At1g26120 and At3g02410, respectively. Results: Bioinformatics investigations showed that the ICME and other two ICME-like homologs comprise a small subfamily of carboxylesterase (EC 3.1.1.1) in Arabidopsis. Sub-cellular localization of GFP tagged ICME and its homologs showed that the ICME and ICME-like proteins are intramembrane proteins predominantly localizing in the endoplasmic reticulum (ER) and Golgi apparatus. Semi-quantitative and real-time quantitative PCR revealed that the ICME and ICME-like genes are expressed in all examined tissues, including roots, rosette leaves, cauline leaves, stems, flowers, and siliques, with differential expression levels. Within the gene family, the base transcript abundance of ICME-LIKE2 gene is very low with higher expression in reproductive organs (flowers and siliques). Time-course analysis uncovered that both ICME and ICME-like genes are up-regulated by mannitol, NaCl and ABA treatment, with ICME showing the highest level of up-regulation by these treatments. Heat stress resulted in up-regulation of the ICME gene significantly but down-regulation of the ICME-LIKE1 and ICME-LIKE2 genes. Cold and dehydration stimuli led to no significant change of both ICME and ICME-like gene expression. Mutant icme-like2-1 showed increased sensitivity to ABA but slightly decreased sensitivity to salt and osmotic stresses during seed germination. Conclusions: It is concluded that the ICME family is involved in stress and ABA signaling in Arabidopsis, probably through mediating the process of demethylating prenylated proteins. Identification of these prenylated proteins will help to better understand the significance of protein prenylation in Planta.
ACCESSION #
55090581

 

Related Articles

  • Overexpression of profilin 3 affects cell elongation and F-actin organization in Arabidopsis thaliana. Fan, Tingting; Zhai, Huanhuan; Shi, Wenwei; Wang, Jue; Jia, Honglei; Xiang, Yun; An, Lizhe // Plant Cell Reports;Jan2013, Vol. 32 Issue 1, p149 

    Key message: Reduced levels of profilin 3 do not have a noticeable phenotypic effect; however, elevated profilin 3 levels result in decreased hypocotyl length due to a reduction in cell elongation and F-actin reorganization. Abstract: The actin cytoskeleton is critical for a variety of cellular...

  • Plants and the Environment. PARF‐1: an Arabidopsis thaliana FYVE‐domain protein displaying a novel eukaryotic domain structure and phosphoinositide affinity. Heras, Begonña; Drøbak, Bjørn K. // Journal of Experimental Botany;Mar2002, Vol. 53 Issue 368, p565 

    A full‐length cDNA encoding a novel protein named PARF‐1 was isolated from Arabidopsis thaliana. PARF‐1 is the first eukaryotic protein to be identified that displays a domain structure which includes a FYVE‐finger domain, a Pleckstrin Homology (PH) domain, as well as...

  • Multiple reference genomes and transcriptomes for Arabidopsis thaliana. Xiangchao Gan; Stegle, Oliver; Behr, Jonas; Steffen, Joshua G.; Drewe, Philipp; Hildebrand, Katie L.; Lyngsoe, Rune; Schultheiss, Sebastian J.; Osborne, Edward J.; Sreedharan, Vipin T.; Kahles, André; Bohnert, Regina; Jean, Géraldine; Derwent, Paul; Kersey, Paul; Belfield, Eric J.; Harberd, Nicholas P.; Kemen, Eric; Toomajian, Christopher; Kover, Paula X. // Nature;9/22/2011, Vol. 477 Issue 7365, p419 

    Genetic differences between Arabidopsis thaliana accessions underlie the plant's extensive phenotypic variation, and until now these have been interpreted largely in the context of the annotated reference accession Col-0. Here we report the sequencing, assembly and annotation of the genomes of...

  • The SET-Domain Protein SUVR5 Mediates H3K9me2 Deposition and Silencing at Stimulus Response Genes in a DNA Methylation--Independent Manner. Caro, Elena; Stroud, Hume; Greenberg, Maxim V. C.; Bernatavichute, Yana V.; Feng, Suhua; Groth, Martin; Vashisht, Ajay A.; Wohlschlegel, James; Jacobsen, Steve E. // PLoS Genetics;Oct2012, Vol. 8 Issue 10, Special section p1 

    In eukaryotic cells, environmental and developmental signals alter chromatin structure and modulate gene expression. Heterochromatin constitutes the transcriptionally inactive state of the genome and in plants and mammals is generally characterized by DNA methylation and histone modifications...

  • Endoplasmic reticulum stress activates the expression of a sub-group of protein disulfide isomerase genes and AtbZIP60 modulates the response in Arabidopsis thaliana. Dong-Ping Lu; David Christopher // Molecular Genetics & Genomics;Sep2008, Vol. 280 Issue 3, p199 

    Abstract  Proteins entering the secretory pathway of eukaryotic cells are folded into their native structures in the endoplasmic reticulum (ER). Disruption of protein folding causes ER stress and activates signaling cascades, designated the unfolded protein response (UPR), that...

  • Diversity and evolution of transposable elements in Arabidopsis. Joly-Lopez, Zoé; Bureau, Thomas // Chromosome Research;Jun2014, Vol. 22 Issue 2, p203 

    Transposable elements are mobile genetic elements that have successfully populated eukaryotic genomes and show diversity in their structure and transposition mechanisms. Although first viewed solely as selfish, transposable elements are now known as important vectors to drive the adaptation and...

  • Intergenic and Genic Sequence Lengths Have Opposite Relationships with Respect to Gene Expression. Colinas, Juliette; Schmidler, Scott C.; Bohrer, Gil; Iordanov, Borislav; Benfey, Philip N. // PLoS ONE;2008, Vol. 3 Issue 11, p1 

    Eukaryotic genomes are mostly composed of noncoding DNA whose role is still poorly understood. Studies in several organisms have shown correlations between the length of the intergenic and genic sequences of a gene and the expression of its corresponding mRNA transcript. Some studies have found...

  • Expression Pattern of Aux/IAA Genes in the iaa3/shy2‐1D Mutant of Arabidopsis thaliana (L.). OONO, YUTAKA; OOURA, CHIHARU; UCHIMIYA, HIROFUMI // Annals of Botany;Jan2002, Vol. 89 Issue 1, p77 

    A semi‐dominant mutant suppressor of hy2 (shy2‐1D) of Arabidopsis thaliana, originally isolated as a photomorphogenesis mutant, shows altered auxin responses. Recent molecular cloning revealed that the SHY2 gene is identical to the IAA3 gene, a member of the primary...

  • GAL4-GFP enhancer trap lines for genetic manipulation of lateral root development in Arabidopsis thaliana. Laplaze, Laurent; Parizot, Boris; Baker, Andrew; Ricaud, Lilian; Martinière, Alexandre; Auguy, Florence; Franche, Claudine; Nussaume, Laurent; Bogusz, Didier; Haseloff, Jim // Journal of Experimental Botany;Sep2005, Vol. 56 Issue 419, p2433 

    Lateral root development occurs throughout the life of the plant and is responsible for the plasticity of the root system. In Arabidopsis thaliana, lateral root founder cells originate from pericycle cells adjacent to xylem poles. In order to study the mechanisms of lateral root development, a...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics