TITLE

Comparison of 454-ESTs from Huperzia serrata and Phlegmariurus carinatus reveals putative genes involved in lycopodium alkaloid biosynthesis and developmental regulation

AUTHOR(S)
Hongmei Luo; Ying Li; Chao Sun; Qiong Wu; Jingyuan Song; Yongzhen Sun; Steinmetz, André; Shilin Chen
PUB. DATE
January 2010
SOURCE
BMC Plant Biology;2010, Vol. 10, p209
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Plants of the Huperziaceae family, which comprise the two genera Huperzia and Phlegmariurus, produce various types of lycopodium alkaloids that are used to treat a number of human ailments, such as contusions, swellings and strains. Huperzine A, which belongs to the lycodine type of lycopodium alkaloids, has been used as an anti-Alzheimer's disease drug candidate. Despite their medical importance, little genomic or transcriptomic data are available for the members of this family. We used massive parallel pyrosequencing on the Roche 454-GS FLX Titanium platform to generate a substantial EST dataset for Huperzia serrata (H. serrata) and Phlegmariurus carinatus (P. carinatus) as representative members of the Huperzia and Phlegmariurus genera, respectively. H. serrata and P. carinatus are important plants for research on the biosynthesis of lycopodium alkaloids. We focused on gene discovery in the areas of bioactive compound biosynthesis and transcriptional regulation as well as genetic marker detection in these species. Results: For H. serrata, 36,763 unique putative transcripts were generated from 140,930 reads totaling over 57,028,559 base pairs; for P. carinatus, 31,812 unique putative transcripts were generated from 79,920 reads totaling over 30,498,684 base pairs. Using BLASTX searches of public databases, 16,274 (44.3%) unique putative transcripts from H. serrata and 14,070 (44.2%) from P. carinatus were assigned to at least one protein. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) orthology annotations revealed that the functions of the unique putative transcripts from these two species cover a similarly broad set of molecular functions, biological processes and biochemical pathways. In particular, a total of 20 H. serrata candidate cytochrome P450 genes, which are more abundant in leaves than in roots and might be involved in lycopodium alkaloid biosynthesis, were found based on the comparison of H. serrata and P. carinatus 454-ESTs and real-time PCR analysis. Four unique putative CYP450 transcripts (Hs01891, Hs04010, Hs13557 and Hs00093) which are the most likely to be involved in the biosynthesis of lycopodium alkaloids were selected based on a phylogenetic analysis. Approximately 115 H. serrata and 98 P. carinatus unique putative transcripts associated with the biosynthesis of triterpenoids, alkaloids and flavones/flavonoids were located in the 454-EST datasets. Transcripts related to phytohormone biosynthesis and signal transduction as well as transcription factors were also obtained. In addition, we discovered 2,729 and 1,573 potential SSR-motif microsatellite loci in the H. serrata and P. carinatus 454-ESTs, respectively.
ACCESSION #
55090564

 

Related Articles

  • Lyconadins G and H, Two Rare Lyconadin-Type Lycopodium Alkaloids from Lycopodium complanatum. Jin-Tang Cheng; Zhi-Jun Zhang; Xiao-Nian Li; Li-Yan Peng; Huai-Rong Luo; Xing-De Wu; Qin-Shi Zhao // Natural Products & Bioprospecting;Dec2016, Vol. 6 Issue 6, p279 

    Two rare lyconadin-type Lycopodium alkaloids, lyconadins G (1) and H (2), together with four known ones (3- 6), were isolated from Lycopodium complanatum. The structures were determined on the basis of their spectroscopic analyses, and the absolute configuration of 1 was established by an X-ray...

  • A new and fast method to obtain in vitro cultures of Huperzia selago (Huperziaceae) sporophytes, a club moss which is a source of huperzine A. Szypuła, Wojciech J.; Mistrzak, Paulina; Olszowska, Olga // Acta Societatis Botanicorum Poloniae;2013, Vol. 82 Issue 4, p313 

    This study presents a protocol for a fast and effective in vitro axenic culture of Huperzia selago (Huperziaceae Rothm.) sporophytes, a club moss which is a source of huperzine A, an alkaloid of a considerable therapeutic potential extensively investigated for its uses as treatment for some...

  • Enhanced colchicine production in root cultures of Gloriosa superba by direct and indirect precursors of the biosynthetic pathway. Ghosh, Biswajit; Mukherjee, Swapna; Jha, Timir B.; Jha, Sumita // Biotechnology Letters;Feb2002, Vol. 24 Issue 3, p231 

    Excised root cultures of Gloriosa superba reached 7.5 g dry wt l-1 and accumulated 240±40 μg colchicine g-1 cell dry wt after 4 weeks growth. While all precursors (except trans-cinnamic acid) enhanced colchicine content of root cultures without adversely affecting root growth, treatment...

  • Holocene Vegetation, Savanna Origins and Human Settlement of Guam. Athens, J. Stephen; Ward, Jerome V. // Records of the Australian Museum;5/19/2004 Supplement 29, p15 

    Palaeoenvironrnental investigations not only provide information about past climate, geomorphological changes, and vegetation, but also can give a unique and complementary perspective to archaeological studies relating to the history of human settlement, The IARII Laguas core on the west coast...

  • Huperzines S, T, and U: New Lycopodium alkaloids from Huperzia serrata. Chang-Heng Tan; Xiao-Qiang Ma; Guo-Fu Chen; Da-Yuan Zhu // Canadian Journal of Chemistry;Apr2003, Vol. 81 Issue 4, p315 

    Three new Lycopodium alkaloids, huperzines S (1), T (2), and U (3), along with eight known compounds, were isolated from the total alkaloids of the whole plant of Huperzia serrata (Thunb) Trev. (Huperziaceae). Those novel alkaloids were identified as 213,1313-epoxyalopecuridine (1),...

  • Efficient strategy for maintaining and enhancing the huperzine A production of Shiraia sp. Slf14 through inducer elicitation. Yan, Riming; Zhang, Zhibin; Wang, Ya; Yang, Huilin; Zeng, Qinggui; Zhu, Du // Journal of Industrial Microbiology & Biotechnology;Jul2014, Vol. 41 Issue 7, p1175 

    Huperzine A (HupA), a naturally occurring lycopodium alkaloid, is a potent, highly specific and reversible inhibitor of acetylcholinesterase and is a potential treatment for Alzheimer's disease. However, isolating HupA from Huperziaceae plants is inefficient; thus, extracting this compound from...

  • A new lycopodine alkaloid from Huperzia serrata. Shan, Wei-Guang; Ren, Feng-Yun; Ying, You-Min; Tong, Cui-Ping; Zhan, Zha-Jun // Journal of Chemical Research;2012, Vol. 36 Issue 1, p15 

    A new lycopodine alkaloid, 6α, 11α-dihydroxylycopodine, and three known compounds L20, lycoposerramine K, and huperzine A were isolated from the stems of Huperzia serrata. The structure of the new alkaloid was established by spectroscopic methods, especially two-dimensional NMR techniques.

  • Anti-Cholinesterase Activity of Lycopodium Alkaloids from Vietnamese Huperzia squarrosa (Forst.) Trevis. Nguyen Ngoc Chuong; Nguyen Thi Thu Huong; Tran Manh Hung; Tran Cong Luan // Molecules;2014, Vol. 19 Issue 11, p19172 

    A series of Lycopodium alkaloids, namely lycosquarosine A (1), acetylaposerratinine (2), huperzine A (3), huperzine B (4), 8α-hydrophlemariurine B (5), and huperzinine (6), has been isolated from Vietnamese Huperzia squarrosa. Among them, lycosquarosine A (1) is the new metabolite of the...

  • Effects of different elicitors on yield of tropane alkaloids in hairy roots of Anisodus acutangulus. Kai, Guoyin; Yang, Sheng; Zhang, Yan; Luo, Xiuqin; Fu, Xueqing; Zhang, Ang; Xiao, Jianbo // Molecular Biology Reports;Feb2012, Vol. 39 Issue 2, p1721 

    The four tropane alkaloids have played a pivotal role in controlling diseases such as the toxic and septic shock, the organophosphorus poison and the acute lung injury. Here, the elicitation effect of different elicitors on the production of tropane alkaloids and the molecular mechanism of...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics