TITLE

Multiple induced mutagenesis for improvement of ethanol production by Kluyveromyces marxianus

AUTHOR(S)
Zong-wen Pang; Jing-juan Liang; Xiao-juan Qin; Ji-rui Wang; Jia-xun Feng; Ri-bo Huang
PUB. DATE
December 2010
SOURCE
Biotechnology Letters;Dec2010, Vol. 32 Issue 12, p1847
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Kluyveromyces marxianus GX-15 was mutated multiple times by alternately treatment with UV irradiation and NTG for two cycles. Four mutant strains with improved ethanol yield were obtained. The maximum ethanol concentration, ethanol yield coefficient and theoretical ethanol yield of the best mutant strain, GX-UN120, was 69 g/l, 0.46 g/g and 91%, respectively, when fermenting 150 g glucose/l at 40°C. The corresponding values for GX-15 were 58 g/l, 0.39 g/g and 76%, respectively. GX-UN120 grew well in 11% (v/v) of ethanol, while GX-15 could not grow when ethanol was greater than 8% (v/v).
ACCESSION #
55024586

 

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