Phenotypic profiling of keloid scars using FT-IR microspectroscopy reveals a unique spectral signature

Hollywood, Katherine A.; Maatje, Marlies; Shadi, Iqbal T.; Henderson, Alex; McGrouther, Duncan; Goodacre, Royston; Bayat, Ardeshir
December 2010
Archives of Dermatological Research;Dec2010, Vol. 302 Issue 10, p705
Academic Journal
Keloid disease (KD) is a quasineoplastic fibroproliferative tumour of unknown origin causing a progressive, recurrent dermal lesion. KD is not homogeneous in nature and shows phenotypic structural differences between its distinct peripheral margins compared to its centre. The keloid margin is often symptomatically more active with increased dermal cellularity, compared to a symptomatically dormant and hypocellular centre of lesion. The aim of this study was to delineate the morphological components of a keloid scar tissue by measuring the differences between various anatomical locations within the keloid tissue, such as the margin and the centre of the lesion compared to its surrounding normal skin using Fourier transform infrared (FT-IR) microspectroscopy. FT-IR microspectroscopy is a technique that produces spectra with detailed molecular biochemical information inherent of the chemical structure. Chemical maps were constructed on extralesional cross sections taken from six keloid scars. H&E stained sections were used to confirm diagnosis of keloid and orientate the experimental cross sections prior to FT-IR. Spectral band assignment and principal components analysis were conducted. Distinct vibrational bands (100 spectra) were observed using FT-IR spectroscopy. Partial least squares discriminant analysis, with bootstrapping (10,000 analyses), identified whether a spectrum was from the keloid or normal tissue showing an average accuracy of 84.8%, precision of 80.4%, specificity of 76.2%, and sensitivity of 92.9%. FT-IR microspectroscopy showed significant differences in spectral profiles in keloid tissue in different anatomical locations within the cross section. We believe that this proof-of-concept study may help substantiate the use of FTIR spectroscopy in keloid diagnosis and prognosis.


Related Articles

  • Trends of HCl, ClONO2 and HF column abundances from ground-based FTIR measurements in Kiruna (Sweden) in comparison with KASIMA model calculations. Kohlhepp, R.; Barthlott, S.; Blumenstock, T.; Hase, F.; Kaiser, I.; Raffalski, U.; Ruhnke, R. // Atmospheric Chemistry & Physics Discussions;2011, Vol. 11 Issue 1, p1489 

    Trends of hydrogen chloride (HCl), chlorine nitrate (ClONO2) and hydrogen fluoride (HF) column abundances above Kiruna (Northern Sweden, 67.84° N, 20.41° E) derived from nearly 14 years (1996--2009) of measurement and model data are presented. The measurements have been performed with a...

  • Renewed methane increase for five years (2007-2011) observed by solar FTIR spectrometry. Sussmann, R.; Forster, F.; Rettinger, M.; Bousquet, P. // Atmospheric Chemistry & Physics Discussions;2011, Vol. 11 Issue 11, p30757 

    Trends of column-averaged methane for the time period [1996, September 2011] are derived from the mid-infrared (mid-IR) solar FTIR time series at the Zugspitze (47.42° N, 10.98° E, 2964 ma.s.l.) and Garmisch (47.48° N, 11.06° E, 743 ma.s.l.). Trend analysis comprises a fit to the...

  • Molecular typing of Cryptococcus neoformans by PCR fingerprinting, in comparison with serotyping and Fourier transform infrared-spectroscopy-based phenotyping. Lemmer, K.; Naumann, D.; Raddatz, B.; Tintelnot, K. // Medical Mycology;Apr2004, Vol. 42 Issue 2, p135 

    Molecular typing by PCR fingerprinting using the single primer (GACA) 4 was performed with 110 isolates of Cryptococcus neoformans . Seventy clinical isolates of C. neoformans var. neoformans from Germany ( n =52) and Africa ( n =18) were included. Of these, serotype A ( C. neoformans var....

  • Cisplatin Resistant Patterns in Ovarian Cell Line Using FTIR and Principle Component Analysis. Zendehdel, Rezvan; Masoudi-Nejad, Ali; Mohammadzadeh, Javad; Shirazi, Farshad H. // Iranian Journal of Pharmaceutical Research;Jan2012, Vol. 11 Issue 1, p235 

    Cisplatin is a common chemotherapeutic agent that used for treatment of many solid cancers. Rapid identification of chemotherapy resistance is very important and may lead to effective treatment plan. Spectroscopy techniques, such as infrared spectroscopy, which are sensitive to biochemical...

  • FTIR spectro-imaging of collagen scaffold formation during glioma tumor development. Noreen, Razia; Chien, Chia-Chi; Chen, Hsiang-Hsin; Bobroff, Vladimir; Moenner, Michel; Javerzat, Sophie; Hwu, Yeukuang; Petibois, Cyril // Analytical & Bioanalytical Chemistry;Nov2013, Vol. 405 Issue 27, p8729 

    Evidence has recently emerged that solid and diffuse tumors produce a specific extracellular matrix (ECM) for division and diffusion, also developing a specific interface with microvasculature. This ECM is mainly composed of collagens and their scaffolding appears to drive tumor growth. Although...

  • Intra-population variation and geographic correlation in Canthon humectus hidalgoensis using FTIR-ATR spectroscopy. Verdú, José; Gallego, Belén; Lobo, Jorge; Ortiz, Antonio; Cortez, Vieyle; Halffter, Gonzalo // Ecological Research;Nov2014, Vol. 29 Issue 6, p1105 

    In some cases external morphology is not sufficient to discern between populations of a species, as occurs in the dung beetle Canthon humectus hidalgoensis Bates; and much less to determine phenotypic distances between them. FTIR-ATR spectroscopy show several advantages over other identification...

  • Identification of Trueperella pyogenes Isolated from Bovine Mastitis by Fourier Transform Infrared Spectroscopy. Nagib, Samy; Rau, Jörg; Sammra, Osama; Lämmler, Christoph; Schlez, Karen; Zschöck, Michael; Prenger-Berninghoff, Ellen; Klein, Guenter; Abdulmawjood, Amir // PLoS ONE;Aug2014, Vol. 9 Issue 8, p1 

    The present study was designed to investigate the potential of Fourier transform infrared (FT-IR) spectroscopy to identify Trueperella (T.) pyogenes isolated from bovine clinical mastitis. FT-IR spectroscopy was applied to 57 isolates obtained from 55 cows in a period from 2009 to 2012. Prior to...

  • Phenotypic and Genotypic Characteristics of Members of the Genus Streptobacillus. Eisenberg, Tobias; Nicklas, Werner; Mauder, Norman; Rau, Jörg; Contzen, Matthias; Semmler, Torsten; Hofmann, Nicola; Aledelbi, Khayrieh; Ewers, Christa // PLoS ONE;8/7/2015, Vol. 10 Issue 8, p1 

    The genus Streptobacillus (S.) remained monotypic for almost 90 years until two new species were recently described. The type species, S. moniliformis, is one of the two etiological agents of rat bite fever, an under-diagnosed, worldwide occurring zoonosis. In a polyphasic approach field...

  • A biospectroscopic analysis of human prostate tissue obtained from different time periods points to a trans-generational alteration in spectral phenotype. Theophilou, Georgios; Lima, Kássio M. G.; Briggs, Matthew; Martin-Hirsch, Pierre L.; Stringfellow, Helen F.; Martin, Francis L. // Scientific Reports;8/28/2015, p13465 

    Prostate cancer is the most commonly-diagnosed malignancy in males worldwide; however, there is marked geographic variation in incidence that may be associated with a Westernised lifestyle. We set out to determine whether attenuated total reflection Fourier-transform infrared (ATR-FTIR) or Raman...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics