TITLE

Development to the Blastocyst Is Improved by the Injection of Donor Cytoplasm into In Vitro Matured Mouse Oocytes

AUTHOR(S)
Junk, Stephen M.; Dharmarajan, Arun M.; Yovich, John L.
PUB. DATE
September 2001
SOURCE
Reproductive Technologies;Sep2001, Vol. 10 Issue 5, p256
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The purpose of this study was to determine whether the blastocyst development rate of in vitro matured oocytes using a mouse model could be improved by supplementing with donor cytoplasm from in vivo matured oocytes. Three treatment groups were used: control, consisting of in vivo matured oocytes; in vitro matured (IVM) controls, where oocytes were matured in vitro; and IVM treated, receiving donor cytoplasm. The cytoplasmic transfer was performed by aspirating cytoplasm from control oocytes and injecting the cytoplasm into IVM metaphase II oocytes. The oocytes from all three groups were inseminated, and the fertilization and blastocyst development rates were assessed. Fertilization of control oocytes (121/142, 85.2%) was significantly higher than both the IVM control group (112/165, 67.9%; p < .008) and the IVM treated group (56/84, 66.7%; p < .016). The rate of blastocyst development was significantly reduced in the IVM control group (26/197, 24.3%; p < .001) compared with the in vivo matured oocytes (92/121, 76%), but the treated IVM oocytes had a normal rate of blastocyst development (38/56, 67.9%). This study has demonstrated that cytoplasmic transfer from in vivo matured oocytes to IVM oocytes can improve the rate of blastocyst development. Factors within the cytoplasm would therefore appear to be contributing to the developmental potential of the oocyte prior to fertilization.
ACCESSION #
5488357

 

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