Development to the Blastocyst Is Improved by the Injection of Donor Cytoplasm into In Vitro Matured Mouse Oocytes

Junk, Stephen M.; Dharmarajan, Arun M.; Yovich, John L.
September 2001
Reproductive Technologies;Sep2001, Vol. 10 Issue 5, p256
Academic Journal
The purpose of this study was to determine whether the blastocyst development rate of in vitro matured oocytes using a mouse model could be improved by supplementing with donor cytoplasm from in vivo matured oocytes. Three treatment groups were used: control, consisting of in vivo matured oocytes; in vitro matured (IVM) controls, where oocytes were matured in vitro; and IVM treated, receiving donor cytoplasm. The cytoplasmic transfer was performed by aspirating cytoplasm from control oocytes and injecting the cytoplasm into IVM metaphase II oocytes. The oocytes from all three groups were inseminated, and the fertilization and blastocyst development rates were assessed. Fertilization of control oocytes (121/142, 85.2%) was significantly higher than both the IVM control group (112/165, 67.9%; p < .008) and the IVM treated group (56/84, 66.7%; p < .016). The rate of blastocyst development was significantly reduced in the IVM control group (26/197, 24.3%; p < .001) compared with the in vivo matured oocytes (92/121, 76%), but the treated IVM oocytes had a normal rate of blastocyst development (38/56, 67.9%). This study has demonstrated that cytoplasmic transfer from in vivo matured oocytes to IVM oocytes can improve the rate of blastocyst development. Factors within the cytoplasm would therefore appear to be contributing to the developmental potential of the oocyte prior to fertilization.


Related Articles

  • Successful Birth After Transfer of Blastocysts Derived from Oocytes of Unstimulated Woman with Regular Menstrual Cycle After IVM Approach. Weon-Young Son; Sun-Jin Park; Chang-Seop Hyun; Won-Don Lee; San-Hyun Yoon; JIn-Ho Lim // Journal of Assisted Reproduction & Genetics;Nov2002, Vol. 19 Issue 11, p541 

    Reports on a delivery after transfer of blastocysts derived from eggs collected following in vivo HCG priming in a patient with regular menstrual cycles undergoing in vitro maturation (IVM) program. Mature oocytes fertilized by conventional in vitro fertilization; Transfer of three expanded...

  • The in vitro development of mouse embryos beyond the blastocyst stage into the hatching and... Drakakis, P.; Loutradis, D. // Journal of Assisted Reproduction & Genetics;Nov96, Vol. 13 Issue 10, p786 

    Investigates the effect of male and female serum (MS/FS) supplementation on the in vitro development of mouse embryos beyond the blastocyst stage. Effect of bovine serum albumin, MS/FS supplementation of in vitro development of the embryos; In vitro development until the blastocyst stage with...

  • The Maturity of Human Cumulus-Free Oocytes is Positively Related to Blastocyst Development and Viability. Fu-Jen Huang; Hsuan-Wei Huan; Kuo-Chung Lan; Fu-Tsai Kung; Yi-Chi Lin; Hsueh-Wen Chang; Shiuh-Young Chang // Journal of Assisted Reproduction & Genetics;Dec2002, Vol. 19 Issue 12, p555 

    Examines the effects of removing cumulus/corona cells on embryo outcome in vitro. Fertilization rate; Changes in the development of oocytes in the blastocyst stage; Importance of selecting good quality blastocytes for embryo transfer.

  • Comparison of Blastocyst Formation and Pregnancy Rates.  // Fertility Weekly;5/5/2003, p5 

    Examines the effect of intracytoplasmic sperm injection (ICSI) on blastocyst formation and pregnancy rates. Likelihood of conceiving a viable pregnancy; Comparison of ICSI to in vitro fertilization; Reduction in vitro blastulation rate after ICSI.

  • Strategies for embryo utilization in assisted reproduction--how do we assess their relative.... Edgar, David H.; Speirs, Andrew L. // Journal of Assisted Reproduction & Genetics;Oct99, Vol. 16 Issue 9, p460 

    Examines the efficacy of assisted reproduction technology on embryo utilization. Impact of in vitro fertilization (IVF) on pregnancy rate; Blastocyst transfer as an option in human IVF; Rationale for an extended culture using a cell-free system; Efficiency of blastocyst freezing before an...

  • Assessment of maximal fertilization rates with intracytoplasmic sperm injection. Sai Ma; Basil Ho Yuen // Journal of Assisted Reproduction & Genetics;Feb2000, Vol. 17 Issue 2, p80 

    Examines maximal fertilization rates following intracytoplasmic sperm injection (ICSI) in Canada. Utilization of immobilization of sperm and aspiration of oocyte cytoplasm procedures in ICSI fertilization; Comparison between natural fertilization and ICSI; Evaluation of sperm concentration and...

  • Sequential transfer of day 3 embryos and blastocysts after previous IVF failures despite adequate ovarian response. Machtinger, Ronit; Dor, Jehoshua; Margolin, Matbeii; Levron, Jacob; Baum, Micha; Ferber, Betty; Shulman, Adrian; Bider, David; Seidman, Daniel S. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Sep2006, Vol. 13 Issue 3, p376 

    The purpose of this study was to compare IVF outcome following sequential embryo transfer (ET) with that following the transfer of early cleavage embryos among patients with previous multiple IVF failures but adequate ovarian response. A retrospective matched case-control analysis was made of...

  • Comparable effectiveness using flexible single-dose GnRH antagonist (cetrorelix) and single-dose long GnRH agonist (goserelin) protocol for IVF cycles -- a prospective, randomized study. Vlaisavljevic, Veljko; Reljic, Milan; Lovrec, Vida Gavric; Kovacic, Borut // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Oct2003, Vol. 7 Issue 3, p301 

    This prospective randomized study compared the effectiveness of a flexible single-dose gonadotrophin-releasing hormone (GnRH) antagonist (cetrorelix) and a single-dose long GnRH agonist (goserelin) protocol for ovarian stimulation in IVF/intracytoplasmic sperm injection (ICSI) cycles. All...

  • Picking winners. Conger, Krista // Stanford Medicine;Winter2011, Vol. 28 Issue 1, p6 

    The article presents a study on the technique which could predict whether a human embryo will develop successfully, within two days after its fertilization. The study uses time-lapse video and computer software in tracking the development of a subset of 242 frozen, one-cell human embryos from an...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics