Succession of Bacterial Community Inhabited Acid Mine Drainage under High Fe(II) Concentration

Wan, M. X.; Yang, Y.; Zeng, W. M.; Xia, J. L.; Liu, X. D.; Qin, W. Q.; Qiu, G. Z.
August 2010
Journal of Environmental Science & Engineering;Aug2010, Vol. 4 Issue 8, p46
Academic Journal
To reveal the effects of Fe2+ on bacterial communities in the early stages of minerals dissolution, two different acid minedrainage (AMD) samples were collected at Dabaoshan Mine and Shenbu Mine. Community successions of AMD niches were analyzed by Amplified Ribosomal DNA Restriction Analysis (ARDRA), sequencing, and phylogenetic analysis in original AMD samples and their subculture under Fe2+ concentrations. Although geochemical properties and community structures were greatly different between the two original AMD samples, bacterial community successions were still very similar under high Fe2+ concentrations. The results showed that Acidithiobacillus ferrooxidans have competitive relationship with other bacterial species living in the AMD, including species that were also capable of oxidizing ferrous ion. A competitive relationship among different At. ferrooxidans strains likewise existed. Some of At. ferrooxidans can grow first under conditions of high ferrous ion concentration, and other At. ferrooxidans species decreased gradually and disappeared. This suggested that these species of At. ferrooxidans are most acidophilic bacteria and afford Fe3+ to leach other metallic ion in the early stages of minerals dissolution.


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