TITLE

Does Short Duration of Sperm-Oocyte Incubation Increase Viability of Embryos in Human In Vitro Fertilization?

AUTHOR(S)
Lalic, Irena; Catt, James W.; Henman, Michael J.; Muir, Sally
PUB. DATE
March 2001
SOURCE
Reproductive Technologies;Mar2001, Vol. 10 Issue 2, p062
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: This study was conducted to clarify the conflicting reports of the benefits and disadvantages of decreasing the time of oocyte-sperm interaction in human in vitro fertilization (IVF). Methods: A controlled prospective study was undertaken on 502 IVF cases to determine if there was an advantage conferred by short-term exposure (1 hour) of oocytes to spermatozoa. Results were compared to the standard IVF method of overnight (16-18 hours) co-culture of oocytes with spermatozoa. A follow-up study was conducted on 263 cases that used thawed embryos derived from the initial study to estimate overall viability of embryos produced by the two methods. All data were analyzed by the chi-squared test. Results: In stimulated IVF cycles, statistically significant differences were not detected in any of the outcome measures except cleavage rate. The cleavage rate was higher in the short exposure group (p = .040). In cycles using cryopreserved embryos, statistically significant differences were not detected in the outcome measures. Conclusions: Most outcome measures were slightly higher in the short exposure group, indicating that there are no detrimental effects from reducing the time of oocyte-spermatozoa interaction to 1 hour. Although there were no statistically significant differences detected between the different methods, it may be beneficial to reduce the time of oocyte-spermatozoa interaction because of the potential for damage to cell membranes by reactive oxygen species. We propose that the degree of benefit from short-term exposure of oocytes to spermatozoa depends on the culturing conditions (e.g., type of culture media, oxygen concentration present, presence of antioxidants) and the concentration of sperm used for insemination.
ACCESSION #
5484757

 

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