TITLE

Rapid and sensitive RP-LC method with amperometric detection for pharmacokinetic assessment of propafenone in human serum of healthy volunteers

AUTHOR(S)
Chmielewska, A.; Konieczna, L.; Plenis, A.; Baczek, T.; Lamparczyk, H.
PUB. DATE
November 2010
SOURCE
Journal of Analytical Chemistry;Nov2010, Vol. 65 Issue 11, p1164
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
rapid and sensitive liquid chromatography method with amperometric detection has been developed for the determination of propafenone in serum. Sample preparation based on single extraction with dichloromethane using bupivacaine hydrochloride as internal standard. The compounds were separated on C-18 reversed-phase analytical column with the mobile phase composed of methanol-acetonitrile-10 mM KHPO (45: 25: 30, v/v/v). Analytes were detected electrochemically with the use of amperometric detector. The quantification limit for propafenone in serum was 10 ng/mL. Linearity of the method was confirmed in the range of 10-500 ng/mL with correlation coefficient of 0.9998. Inter-day relative standard deviations (RSD) ranged from 0.27 to 11.9% and intra-day RSD equalled from 1.1 to 9.7%. The newly developed method was applied for the monitoring of the drug in blood levels with 18 healthy volunteers taking tablet with propafenone.
ACCESSION #
54552978

 

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