TITLE

Functional FeO@ZnO magnetic nanoparticle-assisted enrichment and enzymatic digestion of phosphoproteins from saliva

AUTHOR(S)
Wei-Yu Chen; Yu-Chie Chen
PUB. DATE
November 2010
SOURCE
Analytical & Bioanalytical Chemistry;Nov2010, Vol. 398 Issue 5, p2049
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Saliva contains various proteins, particularly abundant are phosphoproteins, that may be related to disease occurrences and that play significant roles in a biological system. Thus, medical diagnostics will benefit tremendously if disease-related protein biomarkers are discovered from saliva. In this paper, we propose and demonstrate an approach using functional zinc oxide coated iron oxide magnetic nanoparticles (FeO@ZnO MNPs) as affinity probes to selectively enrich phosphoproteins from complex saliva samples and as microwave absorbers to assist the enrichment and subsequent tryptic digestion of trapped proteins under microwave heating. The target species trapped by MNPs were characterized by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) combined with protein database search. Entire analysis time was shortened to less than 20 min. The detection limit of this approach for a monophosphopeptide was as low as 250 pM (10 μL). [Figure not available: see fulltext.]
ACCESSION #
54552755

 

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