The illustrated life cycle of Microbotryum on the host plant Silene latifolia

Schäfer, Angela Maria; Kemler, Martin; Bauer, Robert; Begerow, Dominik
October 2010
Botany;Oct2010, Vol. 88 Issue 10, p875
Academic Journal
The plant-parasitic genus Microbotryum (Pucciniomycotina) has been used as a model for various biological studies, but fundamental aspects of its life history have not been documented in detail. The smut fungus is characterized by a dimorphic life cycle with a haploid saprophytic yeast-like stage and a dikaryotic plant-parasitic stage, which bears the teliospores as dispersal agents. In this study, seedlings and flowers of Silene latifolia Poir. (Caryophyllaceae) were inoculated with teliospores or sporidial cells of Microbotryum lychnidis-dioicae (DC. ex Liro) G. Deml & Oberw. and the germination of teliospores, the infection process, and the proliferation in the host tissue were documented in vivo using light and electron microscopy. Although germination of the teliospore is crucial for the establishment of Microbotryum, basidium development is variable under natural conditions. In flowers, where the amount of nutrients is thought to be high, the fungus propagates as sporidia, and mating of compatible cells takes place only when flowers are withering and nutrients are decreasing. On cotyledons (i.e., nutrient-depleted conditions), conjugation occurs shortly after teliospore germination, often via intrapromycelial mating. After formation of an infectious hypha with an appressorium, the invasion of the host occurs by direct penetration of the epidermis. While the growth in the plant is typically intercellular, long distance proliferation seems mediated through xylem tracheary elements. At the beginning of the vegetation period, fungal cells were found between meristematic shoot host cells, indicating a dormant phase inside the plant. By using different microscopy techniques, many life stages of Microbotryum are illustrated for the first time, thereby allowing new interpretations of laboratory data.


Related Articles

  • Light- and electron-microscopic observations of Cladosporium sp. growing on basidia of Exobasidum camelliae var. gracilis. Mims, C. W.; Hanlin, R. T.; Richardson, E. A. // Canadian Journal of Botany;Jan2007, Vol. 85 Issue 1, p76 

    Basidia of the plant-pathogenic fungus Exobasidium camelliae var. gracilis Shirai became exposed on the abaxial side of an infected leaf of Camellia sasanqua Thunb. following the sloughing of the undersurface of the leaf. Basidia were formed in tremendous numbers in a distinct hymenium that...

  • TOL YPOSPORIUM JUNCI: A NEW SMUT FUNGUS FOR BELARUS.  // Botanica Lithuanica;2011, Vol. 17 Issue 1, p55 

    No abstract available.

  • INVENTARIO DE LAS ASOCIACIONES ÁFIDO-PLANTA EN EL PERÚ. Delfino, Miguel Angel // Ecologia Aplicada;2005, Vol. 4 Issue 1/2, p143 

    Information on aphid-plant associations in Peru, available in many different papers was collected and systematized. Based on the biology of the aphids involved, some of the associations reported in the literature were considered erroneous and set aside. When necessary, the scientific names of...

  • Phytopathogenic fungus Fusarium cerealis in Russia. Gagkaeva, T. Yu. // Microbiology (00262617);Aug2010, Vol. 79 Issue 4, p553 

    The fungus Fusarium cerealis is described, which had not been previously identified in Russia. F. cerealis was isolated from wheat and barley roots and grains, as well as from the leaves of thistle collected in the Far Eastern, North Caucasus, and Central regions. F. cerealis strains may be...

  • Impact of disease frequency and host density on pollination and transmission of an African anther-smut fungus. Curran, Helen; Dreyer, Léanne; Roets, Francois // Planta;Dec2012, Vol. 236 Issue 6, p1677 

    The vast majority of flowering plants rely solely on insects for pollination. A number of pathogens have evolved mechanisms to exploit these close associations and use pollinators as vectors of infective propagules. Factors that affect pollinator movements and successful pollination may in turn...

  • Use of intercellular washing fluid to investigate the secreted proteome of the rice- Magnaporthe interaction. Shenton, Matthew; Berberich, Thomas; Kamo, Masaharu; Yamashita, Tetsuro; Taira, Hideharu; Terauchi, Ryohei // Journal of Plant Research;Mar2012, Vol. 125 Issue 2, p311 

    Early interactions between invading penetration hyphae of the pathogenic fungus Magnaporthe oryzae and rice cells occur at the apoplast, the free diffusional space outside the plasma membrane of leaves. After initial colonization, intercellular hyphae are again in intimate contact with the rice...

  • Major Postharvest Fungal Diseases of Papaya cv. 'Sekaki' in Selangor, Malaysia. Rahman, M. A.; Mahmud, T. M. M.; Kadir, J.; Rahman, R. Abdul; Begum, M. M. // Pertanika Journal of Tropical Agricultural Science;Feb2008, Vol. 31 Issue 1, p27 

    A total of seven fungi were identified from the surface of fully matured papaya fruits cv. 'Sekaki' collected form two different fields namely University Agriculture Park, UPM and MARDI, Selangor and a fruit exporter [Seng Chew Hup Kee (M) Sdn Bhd, Kajang, Selangor, Malaysia]. They were...

  • Ultrastructure and Development of Anthracoidea Elynae Ustilospores. PARVU, Marcel; PARVU, Alina Elena; CRACIUN, Constantin; BARBU-TUDORAN, Lucian; PUSCAS, Mihai // Notulae Botanicae Horti Agrobotanici Cluj-Napoca;2009, Vol. 37 Issue 1, p41 

    The aim of the study was to examine the ultrastructure of Anthracoidea elynae ustilospores isolated from Kobresia myosuroides (Vill.) Fiori plant ovaries, harvested in the Bucegi Mountains, Romania. Samples examination was performed using scanning (SEM) and transmission (TEM) electron...

  • Proteomic Approaches to Understand Trichoderma Biocontrol Mechanisms and Plant Interactions. Hermosa, Rosa; Woo, Sheridan L.; Lorito, Matteo; Monte, Enrique // Current Proteomics;Dec2010, Vol. 7 Issue 4, p298 

    No abstract available.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics