Analysis of local and systemic inflammatory responses induced by polymicrobial peritonitis in mice

Fr�de, T�nia Silvia; Ferreira, Silvia Ines Alejandra Cordoba Pires; Medeiros, Yara Santos
October 2001
Mediators of Inflammation;Oct2001, Vol. 10 Issue 5, p237
Academic Journal
BACKGROUND: Abdominal sepsis induces a local production of proinflammatory mediators that may trigger both septic shock and organ-system dysfunction. Aims: The present study analyzed exudation, cell migration, and CD11a and CD18 subset cells of both local and systemic responses induced by fecal peritonitis in mice. Methods: Animals were anesthetized and, after performing a midline incision in the abdomen, the cecum was ligated and punctured twice with a needle. Sham-operated animals were included. Some groups were previously treated with Evans blue dye (intravenously) to further evaluate the amount of tissue and abdominal cavity leakages. Results: Fecal peritonitis triggered a local inflammatory reaction with an increased number of leukocytes and exudation between 6 and 48 h (p < 0.01). Although CD11a/CD18-positive cells in the abdomen peaked after 24 h, a significant decrease of them was detected after 48 h (p < 0.05). At the studied period of time (6-48 h), different degrees of exudation in several organs occurred, whereas a significant late recruitment (24 h) of CD11a/CD18 cells into the lungs was observed. Conclusions: In this model, cell migration and exudation at the site of injury occurred in parallel. However, in the lungs, the recruitment of leukocytes that express CD11a/CD18 adhesion molecules constitutes a non-dependent event in relation to fluid leakage accumulation at this site.


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