A transcriptomic approach highlights induction of secondary metabolism in citrus fruit in response to Penicillium digitatum infection

González-Candelas, Luis; Alamar, Santiago; Sánchez-Torres, Paloma; Zacarías, Lorenzo; Marcos, Jose F.
January 2010
BMC Plant Biology;2010, Vol. 10, p194
Academic Journal
Background: Postharvest losses of citrus fruit due to green mold decay, caused by the fungus Penicillium digitaum, have a considerable economic impact. However, little is known about the molecular processes underlying the response of citrus fruit to P. digitatum. Results: Here we describe the construction of a subtracted cDNA library enriched in citrus genes preferentially expressed in response to pathogen infection followed by cDNA macroarray hybridization to investigate gene expression during the early stages of colonization of the fruit's peel by P. digitatum. Sequence annotation of clones from the subtracted cDNA library revealed that induction of secondary and amino acid metabolisms constitutes the major response of citrus fruits to P. digitatum infection. Macroarray hybridization analysis was conducted with RNA from either control, wounded, ethylene treated or P. digitatum infected fruit. Results indicate an extensive overlap in the response triggered by the three treatments, but also demonstrated specific patterns of gene expression in response to each stimulus. Collectively our data indicate a significant presence of isoprenoid, alkaloid and phenylpropanoid biosynthetic genes in the transcriptomic response of citrus fruits to P. digitatum infection. About half of the genes that are up-regulated in response to pathogen infection are also induced by ethylene, but many examples of ethylene-independent gene regulation were also found. Two notable examples of this regulation pattern are the genes showing homology to a caffeine synthase and a berberine bridge enzyme, two proteins involved in alkaloid biosynthesis, which are among the most induced genes upon P. digitatum infection but are not responsive to ethylene. Conclusions: This study provided the first global picture of the gene expression changes in citrus fruit in response to P. digitatum infection, emphasizing differences and commonalities with those triggered by wounding or exogenous ethylene treatment. Interpretation of the differentially expressed genes revealed that metabolism is redirected to the synthesis of isoprenes, alkaloids and phenylpropanoids.


Related Articles

  • An Effective Protocol for the Isolation of RNA From the Pulp of Ripening Citrus Fruits. Nengguo Tao; Yunjiang Cheng; Xu, Juan; Qiang Xu; Xiuxin Deng // Plant Molecular Biology Reporter;Sep2004, Vol. 22 Issue 3, p305 

    Isolating RNA is the primary step in the study of gene expression. However, isolating high-quality RNA from ripening citrus fruit tissue is difficult because of the high content of water, polysaccharides, and other secondary metabolites. The traditional protocols for RNA isolation from citrus...

  • The climacteric-like behaviour of young, mature and wounded citrus leaves. Katz, Ehud; Riov, Joseph; Weiss, David; Goldschmidt, Eliezer E. // Journal of Experimental Botany;May2005, Vol. 56 Issue 415, p1359 

    Although leaves and other vegetative tissues are generally considered as non-climacteric, citrus leaves show a climacteric system II behaviour after detachment. Upon harvest, young, fully expanded ‘Valencia’ orange (Citrus sinensis) leaves (∼60-d-old) exhibited two phases of...

  • Identification and characterization of 27 conserved microRNAs in citrus. Changnian Song; Jinggui Fang; Xiaoying Li; Hong Liu; Chao, C. Thomas // Planta;Sep2009, Vol. 230 Issue 4, p671 

    MicroRNAs (miRNAs) are a class of non-protein-coding small RNAs. Considering the conservation of many miRNA genes in different plant genomes, the identification of miRNAs from non-model organisms is both practicable and instrumental in addressing miRNA-guided gene regulation. Citrus is an...

  • Effects of carbohydrate starvation on gene expression in citrus root. Chun Yao Li; Weiss, David; Goldschmidt, Eliezer E. // Planta;May2003, Vol. 217 Issue 1, p11 

    The roots of alternate-bearing citrus (Murcott, a Citrus reticulata hybrid) trees undergo extreme fluctuations of carbohydrate abundance and starvation. Using this system, we investigated the effect of root carbohydrate (total soluble sugar, sucrose and starch) depletion on carbohydrate-related...

  • Profiling gene expression in citrus fruit calyx abscission zone (AZ-C) treated with ethylene. Cheng, Chunzhen; Zhang, Lingyun; Yang, Xuelian; Zhong, Guangyan // Molecular Genetics & Genomics;Oct2015, Vol. 290 Issue 5, p1991 

    On-tree storage and harvesting of mature fruit account for a large proportion of cost in the production of citrus, and a reduction of the cost would not be achieved without a thorough understanding of the mechani sm of the mature fruit abscission. Genome-wide gene expression changes in...

  • Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype. Mafra, Valéria; Martins, Polyana K.; Francisco, Carolina S.; Ribeiro-Alves, Marcelo; Freitas-Astúa, Juliana; Machado, Marcos A. // BMC Genomics;2013, Vol. 14 Issue 1, p1 

    Background: Citrus huanglongbing (HLB) disease is caused by endogenous, phloem-restricted, Gram negative, uncultured bacteria named Candidatus Liberibacter africanus (CaLaf), Ca. L. asiaticus (CaLas), and Ca. L. americanus (CaLam), depending on the continent where the bacteria were first...

  • Transcriptome profile analysis of flowering molecular processes of early flowering trifoliate orange mutant and the wild-type [Poncirus trifoliata (L.) Raf.] by massively parallel signature sequencing. Jin-Zhi Zhang; Xiao-Yan Ai; Lei-Ming Sun; Dong-Liang Zhang; Wen-Wu Guo; Xiu-Xin Deng; Chun-Gen Hu // BMC Genomics;2011, Vol. 12 Issue 1, p63 

    Background: After several years in the juvenile phase, trees undergo flowering transition to become mature (florally competent) trees. This transition depends on the balanced expression of a complex network of genes that is regulated by both endogenous and environmental factors. However,...

  • Development of molecular tools for the mulundocandin producer Aspergillus sydowii: DNA-mediated transformation and reporter gene expression. Schmitt, E. K.; Eilinghoff, B.; Olliger, R.; Decker, H.; Kück, U. // Applied Microbiology & Biotechnology;Apr2002, Vol. 58 Issue 5, p625 

    The echinocandin-type antimycotic mulundocandin and its derivatives are produced by the filamentous fungus Aspergillus sydowii (strain FH2551). These agents have been considered as a potential drug to treat immunocompromised patients who suffer from severe opportunistic fungal infections. In...

  • Ameliorative efficacy of citrus fruit oil in aflatoxicosis in broilers: a growth and biochemical study. DHANAPAL, Senthil KUMAR; RAO, Suguna; GOVINDARAJU, Pradeep KUMAR PALAHALLY; HUKKERI, Ravikumar; MATHESH, Karikalan // Turkish Journal of Veterinary & Animal Sciences;Apr2014, Vol. 38 Issue 2, p207 

    An experiment was conducted to study the efficacy of citrus fruit oil on growth parameters and biochemical parameters of broilers in 4 groups with 2 replicates of 20 chicks in each. Standard broiler diet without aflatoxin was given to group A, basal diet + citrus fruit oil 2.5 g kg-1 to group B,...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics