TITLE

Precise and millidegree stable temperature control for fluorescence imaging: Application to phase transitions in lipid membranes

AUTHOR(S)
Farkas, Elaine R.; Webb, Watt W.
PUB. DATE
September 2010
SOURCE
Review of Scientific Instruments;Sep2010, Vol. 81 Issue 9, p093704
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We present the design of a custom temperature-controlled chamber suitable for water or oil immersion fluorescence microscopy and its application to phase behavior in lipid bilayer vesicles. The apparatus is self-contained and portable, suitable for multiuser microscopy facilities. It offers a higher temperature resolution and stability than any comparable commercial apparatus, on the order of millidegrees. We demonstrate the utility of the system in the study of miscibility transitions in model membranes. The temperature-dependent phase behavior of model membrane systems that display liquid-ordered (Lo) phase coexistence with the liquid-disordered (Ld) phase is relevant to understanding the existence of heterogeneities in biological cell plasma membranes, ubiquitously termed 'lipid rafts.'
ACCESSION #
54049781

 

Related Articles

  • Phase behavior of charged lipid bilayer membranes with added electrolyte. Komura, Shigeyuki; Shirotori, Hisashi; Kato, Tadashi // Journal of Chemical Physics;7/8/2003, Vol. 119 Issue 2, p1157 

    We investigate the phase behavior of the aqueous solution of charged lipid bilayer membranes forming a lamellar structure in the presence of the added electrolyte. The Goldstein-Leibler model [Phys. Rev. A 40, 1025 (1989)] proposed for neutral lipids is extended by taking into account the...

  • Revisiting lipid — general anesthetic interactions (II): Halothane location and changes in lipid bilayer microenvironment monitored by fluorescence. Carnini, Anna; Phillips, Heather A.; Shamrakov, Leanne G.; Cramb, David T. // Canadian Journal of Chemistry;Jul2004, Vol. 82 Issue 7, p1139 

    A universal mechanism for the action of general anesthetics (GA) is not yet available. In this study, we investigated the interaction between halothane and 1,2-dipalmitoyl-sn-3-glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl-sn-3-glycero-3-phosphocholine (DOPC) bilayers labeled with Laurdan,...

  • Influence of Na[sup +],Ca[sup 2+], and D[sub 2]O ions on the kinetics of heterophase density fluctuations in a lipid bilayer. Berg, D. B.; Kharakoz, D. P. // Technical Physics Letters;Nov99, Vol. 25 Issue 11, p887 

    Experimentally measured temperature dependences of the velocity and absorption of ultrasound (7 MHz) were used to calculate the temperature dependences of the effective relaxation time Τ[sub eff] of the nucleation process near the phase-transition temperature T[sub t] in phospholipid...

  • Ultra-Sensitive Fluorescence Reader for Bioanalysis. Hesse, Jan; Sonnleitner, Max; Sch�tz, Gerhard J. // Current Pharmaceutical Biotechnology;Jun2004, Vol. 5 Issue 3, p309 

    Recent advances in the development of new microscopical techniques with single-molecule sensitivity have given access to essentially new types of information on biological systems. In this review, basic methodological concepts of ultra-sensitive microscopy are presented and characterized, with...

  • The redox state influences the interaction of ubiquinones with phospholipid bilayers. Roche, Y.; Peretti, P.; Bernard, Sophie // Journal of Thermal Analysis & Calorimetry;Sep2007, Vol. 89 Issue 3, p867 

    Differential scanning calorimetry (DSC) has been used to study the interaction of ubiquinones (UQn) and their reduced forms, ubiquinols (UQnH2), with dipalmitoylphosphatidylcholine (DPPC) in multilamellar vesicles. The influence of the redox state has been investigated by comparing the effect of...

  • NEUTRON SCATTERING INVESTIGATIONS OF THE LIPID BILAYER STRUCTURE PRESSURE DEPENDENCE. Soloviov, D. V.; Bulavin, L. A.; Gordeliy, V. I.; Gorshkova, Yu. E.; Ivankov, O. I.; Kovalev, Y. S.; Kuklin, A. I.; Nikolaienko, T. Yu. // Nuclear Physics & Atomic Energy;2012, Vol. 13 Issue 1, p83 

    Lipid bilayer structure investigation results obtained with small angle neutron scattering method at the Joint Institute for Nuclear Research IBR-2M nuclear reactor (Dubna, Russia) are presented. Experiment has been performed with small angle neutron scattering spectrometer YuMO, upgraded with...

  • Main phase transition in lipid bilayers: Phase coexistence and line tension in a soft, solvent-free, coarse-grained model. Hömberg, Martin; Müller, Marcus // Journal of Chemical Physics;4/21/2010, Vol. 132 Issue 15, p155104 

    We devise a soft, solvent-free, coarse-grained model for lipid bilayer membranes. The nonbonded interactions take the form of a weighted-density functional, which allows us to describe the thermodynamics of self-assembly and packing effects of the coarse-grained beads in terms of a density...

  • Revisiting lipid — general anesthetic interactions (I): Thinned domain formation in supported planar bilayers induced by halothane and ethanol. Leonenko, Zoya V.; Cramb, David T. // Canadian Journal of Chemistry;Jul2004, Vol. 82 Issue 7, p1128 

    A long-standing question in anesthesia is that of the molecular mechanism. Do anesthetics target proteins or change membrane properties or both? We used temperature-dependent magnetic A/C mode atomic force microscopy (AFM) to study interaction of the volatile anesthetics halothane and ethanol...

  • Molecular-dynamics simulation of a ceramide bilayer. Pandit, Sagar A.; Scott, H. Larry // Journal of Chemical Physics;1/7/2006, Vol. 124 Issue 1, p014708 

    Ceramide is the simplest lipid in the biologically important class of glycosphingolipids. Ceramide is an important signaling molecule and a major component of the strateum corneum layer in the skin. In order to begin to understand the biophysical properties of ceramide, we have carried out a...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics