TITLE

Multi-walled carbon nanotubes as solid-phase extraction adsorbent for the ultra-fast determination of chloramphenicol in egg, honey, and milk by fused-core C18-based high-performance liquid chromatography-tandem mass spectrometry

AUTHOR(S)
Yanbin Lu; Qing Shen; Zhiyuan Dai; Hong Zhang
PUB. DATE
October 2010
SOURCE
Analytical & Bioanalytical Chemistry;Oct2010, Vol. 398 Issue 4, p1819
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
In the present work, a high-performance liquid chromatography-tandem mass spectrometry method has been developed for the residue analysis of chloramphenicol (CAP) in several food matrices. Following the addition of D-CAP as internal standard, egg, honey, and milk were extracted and cleaned by means of solid-phase extraction, utilizing multi-walled carbon nanotubes as sorbent. The extracts were separated on a Halo fused-core C18 column (50 mm × 2.1 mm, 2.7 μm) and quantified by a 4000 Q-trap mass spectrometer equipped with a TurboIonSpray™ interface using electrospray ionization and multiple-reaction monitoring mode. The method validation was performed according to the criteria of Commission Decision 2002/657/EC. The decision limit (CCα) and detection capability (CCβ) of CAP in milk were calculated for m/ z 320.8 > 151.9. Due to the existence of slight signal suppression, quantification was performed by matrix-matched calibration curves, ranging from 0.1 to 100 ng mL, with regression coefficients of 0.9993, 0.9998, and 0.9997 for egg, honey, and milk, respectively. Mean recoveries of the CAP ranged from 95.8% to 102.3%, with the corresponding intra- and inter-day variation (relative standard deviation) less than 7.13% and 8.89%, respectively. The limit of detection and limit of quantification of the method were also reported. This method successfully applied to several food matrixes (egg, honey, and milk) and can serve as a monitoring tool to avoid unacceptable levels of residues of CAP entering the food chain.
ACCESSION #
53840429

 

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