TITLE

Raman spectroscopic imaging for in vivo detection of cerebral brain metastases

AUTHOR(S)
Kirsch, Matthias; Schackert, Gabriele; Salzer, Reiner; Krafft, Christoph
PUB. DATE
October 2010
SOURCE
Analytical & Bioanalytical Chemistry;Oct2010, Vol. 398 Issue 4, p1707
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We report for the first time a proof-of-concept experiment employing Raman spectroscopy to detect intracerebral tumors in vivo by brain surface mapping. Raman spectroscopy is a non-destructive biophotonic method which probes molecular vibrations. It provides a specific fingerprint of the biochemical composition and structure of tissue without using any labels. Here, the Raman system was coupled to a fiber-optic probe. Metastatic brain tumors were induced by injection of murine melanoma cells into the carotid artery of mice, which led to subcortical and cortical tumor growth within 14 days. Before data acquisition, the cortex was exposed by creating a bony window covered by a calcium fluoride window. Spectral contributions were assigned to proteins, lipids, blood, water, bone, and melanin. Based on the spectral information, Raman images enabled the localization of cortical and subcortical tumor cell aggregates with accuracy of roughly 250 μm. This study demonstrates the prospects of Raman spectroscopy as an intravital tool to detect cerebral pathologies and opens the field for biophotonic imaging of the living brain. Future investigations aim to reduce the exposure time from minutes to seconds and improve the lateral resolution.
ACCESSION #
53840423

 

Related Articles

  • Novel probes enhance Raman spectroscopy. Lewotsky, Kristin // Laser Focus World;May96, Vol. 32 Issue 5, p42 

    Focuses on the use of fiberoptic sensors in Raman spectroscopy. Applications; Composite fabrication; Generation of the Raman signal; Elimination of crackling problems.

  • Description and performance of a highly versatile, low-cost fiber-optic confocal Raman microscope. Stellman, C.M.; Booksh, K.S.; Reddic, J.E.; Myrick, M.L. // Review of Scientific Instruments;Jan1996, Vol. 67 Issue 1, p79 

    Discusses the development of a versatile fiber-optic confocal Raman microscope. Application of fiber optics to provide remote capabilities for the microscope and the ability to use multiple excitation sources and detection schemes; Horizontal and vertical resolutions of the instrument.

  • Raman spectroscopy using a fiber optic probe with subwavelength aperture. Din Ping Tsai; Othonos, Andreas // Applied Physics Letters;4/4/1994, Vol. 64 Issue 14, p1768 

    Examines submicron diameter fiber optic probe. Record of Raman spectroscopy with spatial resolution by fiber optic probe; Use of fiber optic probe to convey laser radiation and collect Raman scattering light; Collection of Stokes radiation by fiber optic probe.

  • Combined use of FORS, XRF and Raman spectroscopy in the study of mural paintings in the Aosta Valley (Italy). Appolonia, Lorenzo; Vaudan, Davide; Chatel, Valentina; Aceto, Maurizio; Mirti, Piero // Analytical & Bioanalytical Chemistry;Dec2009, Vol. 395 Issue 7, p2005 

    Mural paintings which decorate the external façade and the internal apsidal wall of a chapel dedicated to St. Maxime and located at Challand St. Victor in the Aosta Valley (Italy) have been analysed with a combined approach involving high-resolution fibre-optic reflectance spectroscopy...

  • Raman system.  // Laser Focus World;Mar2006, Vol. 42 Issue 3, p95 

    The article focuses on the availability of the Dimension-P1 Raman System in different sizes. It uses an LSI Vector Raman Probe, an internal sample compartment. The customizable External Sampling Module with Raman microscopy adaptors allows the user to take a wide variety of spectra measurements.

  • Electrical and Raman characterization of silicon and germanium-filled microstructured optical fibers. Finlayson, C. E.; Amezcua-Correa, A.; Sazio, P. J. A.; Baril, N. F.; Badding, J. V. // Applied Physics Letters;3/26/2007, Vol. 90 Issue 13, p132110 

    Extreme aspect ratio tubes and wires of polycrystalline silicon and germanium have been deposited within silica microstructured optical fibers using high-pressure precursors, demonstrating the potential of a platform technology for the development of in-fiber optoelectronics. Microstructural...

  • Non-invasive and non-destructive micro-XRF and micro-Raman analysis of a decorative wallpaper from the beginning of the 19th century. Castro, Kepa; Pérez-Alonso, Maite; Rodríguez-Laso, María Dolores; Etxebarria, Nestor; Madariaga, Juan Manuel // Analytical & Bioanalytical Chemistry;Feb2007, Vol. 387 Issue 3, p847 

    Non-destructive and non-invasive micro-Raman fibre optic and micro-XRF analyses were performed to study a wallpaper from the beginning of the 19th century. The complementarity of these two non-destructive techniques is shown in this work. The analysed artwork is considered one of the most...

  • Design and microfabrication of a miniature fiber optic probe with integrated lenses and mirrors for Raman and fluorescence measurements. Ngernsutivorakul, Thitaphat; Cipolla, Cynthia; Dugan, Colleen; Jin, Shi; Morris, Michael; Kennedy, Robert; Esmonde-White, Francis // Analytical & Bioanalytical Chemistry;Jan2017, Vol. 409 Issue 1, p275 

    Fiber optics coupled to components such as lenses and mirrors have seen extensive use as probes for Raman and fluorescence measurements. Probes can be placed directly on or into a sample to allow for simplified and remote application of these optical techniques. The size and complexity of such...

  • Physics of cancer: A stretch for any brain. Klopper, Abigail // Nature Physics;Aug2014, Vol. 10 Issue 8, p556 

    The article discusses a study by Katarzyna Pogoda and colleagues which found that the differences between cancerous cells and healthy cells can also arise from the way cells respond to pressure gradients within a tissue, which are particularly large in brain tumours.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics