TITLE

Automating a 96-well microtiter plate assay for identification of AGEs inhibitors or inducers: application to the screening of a small natural compounds library

AUTHOR(S)
Derbré, Séverine; Gatto, Julia; Pelleray, Aude; Coulon, Laurie; Séraphin, Denis; Richomme, Pascal
PUB. DATE
October 2010
SOURCE
Analytical & Bioanalytical Chemistry;Oct2010, Vol. 398 Issue 4, p1747
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
dvanced glycation end-products (AGEs) are involved in the pathogenesis of numerous affections such as diabetes and neurological diseases. AGEs are also implied in various changes in tissues and organs. Therefore, compounds able to break them or inhibit their formation may be considered as potential drugs, dietary supplements, or bioactive additives. In this study, we have developed a rapid and reliable ( Z′ factor calculation) anti-AGEs activity screening based on the overall fluorescence of AGEs. This method was successfully evaluated on known AGEs inhibitors and on a small library of natural compounds, yielding coherent results when compared with literature data.
ACCESSION #
53840417

 

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