Early development of cutaneous cancer revealed by intravital nonlinear optical microscopy

Chun-Chin Wang; Feng-Chieh Li; Wei-Chou Lin; Yang-Fang Chen; Shean-Jen Chen; Sung-Jan Lin; Chen-Yuan Dong
September 2010
Applied Physics Letters;9/13/2010, Vol. 97 Issue 11, p113702
Academic Journal
We performed intravital multiphoton microscopy to image and analyze normal and carcinogen treated skin tissues of nude mice in vivo. Using intravital images and the quantitative pixel to pixel ratiometric processing of multiphoton autofluorescence to second harmonic generation index (MAFSI), we can visualize the interaction between epithelial cells and extracellular matrix. We found that as the imaging depth increases, MAFSI has different distribution in normal and treated cutaneous specimens. Since the treated skin eventually became squamous cell carcinoma, our results show that the physiological changes to mouse skin en route to become cancer can be effectively tracked by multiphoton microscopy.


Related Articles

  • Characteristics of scar margin dynamic with time based on multiphoton microscopy. Zhu, Xiaoqin; Zhuo, Shuangmu; Zheng, Liqin; Jiang, Xingshan; Chen, Jianxin; Lin, Bifang // Lasers in Medical Science;Mar2011, Vol. 26 Issue 2, p239 

    Scar margins dynamic with time were quantitatively characterized using multiphoton microscopy (MPM). 2D large-area and 3D focused images of elastin and collagen at scar margins were obtained to extract quantitative parameters. An obvious boundary was observed at the scar margin, showing altered...

  • Measurement of absorption spectrum of deuterium oxide (D2O) and its application to signal enhancement in multiphoton microscopy at the 1700-nm window. Yuxin Wang; Wenhui Wen; Kai Wang; Peng Zhai; Ping Qiu; Ke Wang // Applied Physics Letters;1/11/2016, Vol. 108 Issue 2, p1 

    1700-nm window has been demonstrated to be a promising excitation window for deep-tissue multiphoton microscopy (MPM). Long working-distance water immersion objective lenses are typically used for deep-tissue imaging. However, absorption due to immersion water at 1700 nm is still high and leads...

  • Eyecup scope–optical recordings of light stimulus-evoked fluorescence signals in the retina. Euler, Thomas; Hausselt, Susanne; Margolis, David J.; Breuninger, Tobias; Castell, Xavier; Detwiler, Peter P.; Denk, Winfried // Pflugers Archiv European Journal of Physiology;Mar2009, Vol. 457 Issue 6, p1393 

    Dendritic signals play an essential role in processing visual information in the retina. To study them in neurites too small for electrical recording, we developed an instrument that combines a multi-photon (MP) microscope with a through-the-objective high-resolution visual stimulator. An...

  • Second and higher harmonics generation with memristive systems. Cohen, Guy Z.; Pershin, Yuriy V.; Di Ventra, Massimiliano // Applied Physics Letters;3/26/2012, Vol. 100 Issue 13, p133109 

    We show that memristive systems can be used very efficiently to generate passively both double and higher frequency harmonics. A technique for maximizing the power conversion efficiency into any given harmonic is developed and applied to a single memristive system and memristive bridge circuits....

  • Application of double femtosecond pulses for plasma harmonic generation. Ganeev, R. A.; Suzuki, M.; Yoneya, S.; Kuroda, H. // Applied Physics Letters;7/28/2014, Vol. 105 Issue 4, p1 

    We demonstrate the harmonic generation of the femtosecond pulses propagated in the vicinity of solid targets. The harmonics were observed in the case of double-pulse scheme while using different solids (metals, nanoparticle, and microparticle powders). The efficiency and cut-off of harmonic...

  • Ferroelectric AgNa(NO2)2 crystals as novel highly efficient nonlinear optical material: Phase matched second harmonic generation driven by a spontaneous and electric field induced polarizations. Sahraoui, B.; Czaplicki, R.; Klöpperpieper, A.; Andrushchak, A. S.; Kityk, A. V. // Journal of Applied Physics;Jun2010, Vol. 107 Issue 11, p113526 

    Paper reports the second harmonic generation (SHG) in ferroelectric AgNa(NO2)2 crystals being driven by the spontaneous and electric field induced polarizations. Obtained results are interpreted within the phenomenological theory which considers the free energy describing the interaction between...

  • NONLINEAR OPTICAL SPECTROSCOPY OF POPC LIPOSOMES WITH CHOLESTEROL. Sharifi, Soheil // Science International;Jul/Aug2014, Vol. 26 Issue 3, p983 

    The surface potential and charged density of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine liposomes with cholesterol is study by using the second harmonic generation. The Gouy-Chapman model is applied to data's to find surface potential of samples. The result shows, the surface potential...

  • Chemical Etching, AFM, Laser Damage Threshold, and Nonlinear Optical Studies of Potential Nonlinear Optical Crystal: Bis (L-Glutamine) Potassium Nitrate. Hanumantharao, Redrothu; Kalainathan, S. // Journal of Chemistry;2013, p1 

    A novel semiorganic nonlinear optical crystal bis (L-glutamine) potassium nitrate (BGPN) grown by slow evaporation technique at ambient temperature. The grown crystal surface has been analyzed by chemical etching and atomic force microscopy (AFM) studies. Amplitude parameters like area...

  • Simultaneous time- and spectrum-resolved multifocal multiphoton microscopy. Liu, L.; Qu, J.; Lin, Z.; Wang, L.; Fu, Z.; Guo, B.; Niu, H. // Applied Physics B: Lasers & Optics;Sep2006, Vol. 84 Issue 3, p379 

    We present a novel multifocal multiphoton microscope that is based on a sampling imaging technique using a microlens array, a prism for two-dimensionalspectral dispersion, and a specially designed streak camera to provide simultaneous time- and spectrum-resolved fluorescence microscopy. We split...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics