Multiplex immunoassays of equine virus based on fluorescent encoded magnetic composite nanoparticles

Guannan Wang; Yuan Gao; Hui Huang; Xingguang Su
September 2010
Analytical & Bioanalytical Chemistry;Sep2010, Vol. 398 Issue 2, p805
Academic Journal
new detection format for multiplexed analysis based on fluorescent encoded magnetic composite nanoparticles is presented. Two kinds of virus were analyzed by this new method: equine influenza virus (EIV) and equine infectious anemia virus (EIAV). Firstly, EIV antigen and EIAV antigen were conjugated to two kinds of fluorescent encoded magnetic composite nanoparticles, while the green-emitting CdTe quantum dots (QDs) were attached to the antibody of EIV and EIAV. Then both green-emitting CdTe QD-labeled antibodies and antigens labeled with fluorescent encoded magnetic composite nanoparticles were used to form an immunoassay system for the detection of EIV and EIAV antigens. The method is time-saving and has higher sensitivity (1.3 ng mL for EIV antigens and 1.2 ng mL for EIAV antigens) than the conventional methods. A competitive immunoassay method based on this analysis system was used to detect EIV and EIAV antigens in spiked serum samples with satisfactory results. [Figure not available: see fulltext.]


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