Evaluation of nucleic acid duplex formation on gold over layers in biosensor fabricated using Czochralski-grown single-crystal silicon substrate

Gopinath, Subash C. B.; Kumaresan, Ramanujam; Awazu, Koichi; Fujimaki, Makoto; Mizuhata, Minoru; Tominaga, Junji; Kumar, Penmetcha K. R.
September 2010
Analytical & Bioanalytical Chemistry;Sep2010, Vol. 398 Issue 2, p751
Academic Journal
With a view to developing an economical and elegant biosensor chip, we compared the efficiencies of biosensors that use gold-coated single-crystal silicon and amorphous glass substrates. The reflectivity of light over a wide range of wavelengths was higher from gold layer coated single-crystal silicon substrates than from glass substrates. Furthermore, the efficiency of reflection from gold layers of two different thicknesses was examined. The thicker gold layer (100 nm) on the single-crystal silicon showed a higher reflectivity than the thinner gold film (10 nm). The formation of a nucleic acid duplex and aptamer–ligand interactions were evaluated on these gold layers, and a crystalline silicon substrate coated with the 100-nm-thick gold layer is proposed as an alternative substrate for studies of interactions of biomolecules. [Figure not available: see fulltext.]


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